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ELECTROPHORESIS EQUIPMENT PCR WORKSTATIONS MUTATION DETECTION PRECAST GELS LABORATORY EQUIPMENT 43Years 1973-2016 Cover art courtesy of Ross Bleckner Leader-Sequence 2002 Paulson Bott Press www.paulsonbottpress.com About our Company... I am delighted to present you with our new catalog. 2016 marks our 43rd year dedicated to manufacturing Electrophoresis and Scientific Instruments. CBS Scientific is based in San Diego California and our products are sold worldwide. Back in the 70s while I was acquiring my Ph.D. in Immunology at UCSD I started making electrophoresis instruments in my garage for my lab. Soon surrounding labs were placing orders and C.B.S. Scientific was formed. Many years later we are still regarded as a premiere manufacturer of Electrophoresis Equipment and our reliability design and quality are unparalleled. Each product is engineered for versatility optimal performance and ease of use. Our large offering of horizontal and vertical electrophoresis systems and wide variety of accessories allows researchers to perform current protocols or easily adapt to changing methodologies. Our instruments are handcrafted from cast acrylic or injection molded when advantageous. In addition we have developed one of the most widely used PCR Workstations in the United States and our Mutation Detection Systems are standard equipment in genetics laboratories around the world. C.B.S. Scientific is also a primary supplier of critical components to some of the world leaders in automated laboratory technology. We also maintain ongoing collaborations with scientists to develop and improve new and existing products. Over the past 43 years our core values have remained the same- to manufacture the best and most versatile products possible. We hope the detailed specifications of each product in our new catalog help you decide which instruments would be best for your lab and we encourage you to use our website for both ordering and a source of reference. Chuck Scott Founder and CSO of C.B.S. Scientific w w w. c b s s c i e n t i f i c . c o m Victor McKnight O.E.M. and Custom Equipment Specialist Kim Carter Vice President Major Accounts Bethany Storevik Sales and Customer Service Jocelyn Scott Marketing and Publications Chuck Scott Ph.D. Technical Specialist and CSO BUYERS SELECTION GUIDE VERTICAL ELECTROPHORESIS SYSTEMS 6-7 HORIZONTAL ELECTROPHORESIS SYSTEMS 8-9 MINI VERTICAL SYSTEMS DUAL COOL MINI-VERTICAL 10-11 DCX-700 Plate size cm 10w x 10h QUADRA MINI-VERTICALS 12-13 QNX-700 Plate size cm 10w x 10h MINI-VERTICALS 10CMW 11.3CMW 14-15 MGV-302 Plate size cm 10w x 8h single MGV-402 Plate size cm 10w x 8h dual MGV-102 Plate size cm 11.3w x 10h single MGV-202 Plate size cm 11.3w x 10h dual WIDE DUAL MINI-VERTICAL 15CMW 16-17 DCX-800 Plate size cm 15w x 10h DCX-800C Plate size cm 15w x 10h DOUBLE-WIDE MINI-VERTICALS 20CMW 18-19 MGV-102-20 Plate size cm 20w x 10h Single MGV-202-20 Plate size cm 20w x 10h Dual MGV-116-20 Plate size cm 20w x 16h Single MGV-216-20 Plate size cm 20w x 16h Dual TRIPLE-WIDE MINI-VERTICALS 33CMW 20-21 MGV-102-33 Plate size cm 33w x 10h Single MGV-202-33 Plate size cm 33w x 10h Dual MGV-116-33 Plate size cm 33w x 16h Single MGV-216-33 Plate size cm 33w x 16h Dual VERTICAL SYSTEMS C.B.S. LITE VERTICALS 16.5CMW FIXED HEIGHT 22-23 SG-125-02 Plate size cm 16.5w x 14.5h SG-160-02 Plate size cm 16.5w x 16h SG-170-02 Plate size cm 16.5w x 17h SG-190-02 Plate size cm 16.5w x 19h SG-200-02 Plate size cm 16.5w x 22h SG-250-02 Plate size cm 16.5w x 28h SG-400-02 Plate size cm 16.5w x 38.7h DSG DUAL VERTICALS 16.5CMW FIXED HEIGHTS 24-25 DSG-125-02 Plate size cm 16.5w x 14.5h DSG-160-02 Plate size cm 16.5w x 16h DSG-170-02 Plate size cm 16.5w x 17h DSG-190-02 Plate size cm 16.5w x 19h DSG-200-02 Plate size cm 16.5w x 22h DSG-250-02 Plate size cm 16.5w x 28h DSG-400-02 Plate size cm 16.5w x 38.7h ADJUSTABLE VERTICALS 16.5CMW 26-27 ASG-250 Plate size cm 16.5w x 14.5h - 38.7h Single DASG-250 Plate size cm 16.5w x 14.5h - 38.7h Dual LARGE FORMAT COOLED 20CMW FIXED HEIGHTS 28-29 DCX-900C Plate size cm 20w x 22h LARGE FORMAT ADJUSTABLE 20CMW 30-31 LSG-400-20 Plate size cm 20w x 22-42h LDASG-400-20 Plate size cm 20w x 22-42h MEGA-GEL HIGH THROUGHPUT VERTICAL ADJUSTABLE HEIGHT 32 C-DASG-400-50 Plate sizescm 50w x 22h or 32h PORE GRADIENT LPE SYSTEM 33 LPE-4003 Plate size cm 8.2w x 8.2h BLOTTING SYSTEMS MINI-BLOTTING SYSTEMS 34-37 EBX-700 Gel size cm 9.3w x 9.3h 34-35 EBU-204 Gel size cm 9.3w x 9.3h 34-35 EBU-402 Gel size cm 20w x 9.5h 36 EBU-302 Gel size cm 32.8w x 9.5h 37 EBU-362 Gel size cm 32.8w x 13h 37 LARGE BLOTTING SYSTEMS 38 EBU-102 Gel size cm 20w x 20h 38 EBU-222 Gel size cm 16w x 21.5h 38 SEMI-DRY BLOTTING SYSTEMS 39 EBU-3000 Gel size cm 10w x 30h EBU-4000 Gel size cm 20w x 20h EBU-6000 Gel size cm 35w x 40h Table of ContentsTable of Contents w w w. c b s s c i e n t i f i c . c o m4 HORIZONTAL ELECTROPHORESIS BASEMETRIX BUFFERS AND LADDERS 40 BaseMetrix Rapid Buffer DNA Ladders MINI-HORIZONTAL SYSTEMS 41-43 MGU-303 Mini-Fast Sub Gel Size cm 10w x 8l GCMHU-102 Gel size cm 5.5w x 8.5l GCMHU-202 Gel size cm 7.5w x 10l MIDI-HORIZONTAL SYSTEMS 44-45 GCMHU-402 Gel size cm 10.5w x 11l GCMHU-502 Gel size cm 14w x 10l GCMHU-602 Gel size cm 14w x 16l FLEXICAST HORIZONTAL SYSTEMS 46-47 WHSE-014 Gel size cm 14w x 20l WMGE-600 Gel size cm 16w x 16l HORIZONTAL SYSTEMS 48-49 GCHSU-014 Gel size cm 14w x 20l GCHSU-020 Gel size cm 20w x 20l GCHSU-030 Gel size cm 20w x 30l GCHSU-040 Gel size cm 20w x 40l ECONO-SUB PRODUCTIVITY PACKAGES 50-51 GCHSE-014T-02 Gel size cm 14w x 20l GCHSE-020T-02 Gel size cm 20w x 20l GCHSE-030T-02 Gel size cm 20w x 30l GCHSE-040T Gel size cm 20w x 40l HIGH-THROUGHPUT HORIZONTAL SYSTEMS 52-53 HSU-2614 Gel size cm 26w x 14l HSU-2626 Gel size cm 26w x 26l HSU-2640 Gel size cm 26w x 40l CONTINUOUSLY ADJUSTABLE GEL CASTERS 54 POWER SUPPLIES 55 EPS-300 X 100-240 VAC 1-300V 1-500mA 90W High Current EPX-250 X 100-240 VAC 2-250V 10-2500mA 200W EPS-600 100-240 VAC 5-600V 1-500mA 120W High Voltage EPS-3000P-II 85-265 VAC 20-3000V 1-400mA 400W OPTIMIZER PCR WORKSTATIONS Dead Air Boxes 56-57 P-030-02 single UV light Dimensions cm 61w x 61h x 76w P-030-202 dual UV light Dimensions cm 61w x 61h x 76w P-036-02 single UV light Dimensions cm 61w x 61h x 91w P-036-202 dual UV light Dimensions cm 61w x 61h x 91w P-048-02 single UV light Dimensions cm 61w x 61h x 122w P-048-202 dual UV light Dimensions cm 61w x 61h x 122w ClearPAGE PRECAST GELS TEO Triethanolamine SDS Gels 10cm x 8cm 10cm x 10cm 15cm x 10cm 58-61 DNANATIVE Tris-Acetate Gels 10cm x 10cm 60 MUTATION DETECTION MUTATION DETECTION DISCUSSION 62-63 CIPHER DGGE DENATURING GRADIENT GEL ELECTROPHORESIS SYSTEM 64-65 DGGEK-1001 2-place Plate size cm 17.7w x 22h DGGEK-2001 2-place Plate size cm 17.7w x 22h DGGEK-2401 4-place Plate size cm 17.7w x 22h DGGEK-4801 8-place Plate size cm 17.7w x 22h CIPHER TTGE TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS SYSTEM 66-67 TTGEK-2001 2-place Plate size cm 17.7w x 22h TTGEK-2401 4-place Plate size cm 17.7w x 22h CIPHER DTSK GENETIC ANALYSIS SYSTEM 68-69 DTSK-2001 2-place Plate size cm 17.7w x 22h DTSK-2401 4-place Plate size cm 17.7w x 22h SEQUENCING EQUIPMENT Adjustable Height Sequencers 20cmw and 33cmw 70-71 Dedicated Height Sequencers 20cmw and 33cmw 72-73 Sequencing Accessories 74-77 LABORATORY EQUIPMENT QuickTrap Vacuum Protection System 78 Pipette Racks Glass Racks 78 Gradient Makers 79 Mini-Peristaltic Pump 79 Incubator Culture Chambers 79 Beta Radiation Safety Equipment 80-82 VERTICALSBLOTTINGHORIZONTALSPOWER SUPPLIES PCR WORKSTATIONS PRECASTGELSMUTATION DETECTIONS SEQUENCINGLABORATORY EQUIPMENT p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 5 Mini-Vertical CatProductGlassPlateSize wxhcm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofWellsTemperatureControl Method CompatibleTank BlottingSystems CompatibleSemi-Dry BlottingSystems GelCastingOptionsRecommendedPowerSupplyCat. PAGEGelWrapMultiGelCaster DCX-700DualCoolMiniVerticalSlab GelBlottingSystem 10x101-2yes0.751.01.5101416FreezerblockBuiltinEBU-204 EBX-700 EBU-4000DCX-CDCX-HCDCX-MHCDCX-MC-4 DCX-MC-10DCX4K DCX10K EPS-300X10-11 QNX-700QuadraMiniVerticalSlab GelBlottingSystem 10x101-4yes0.751.01.5101416noBuiltinEBU-204 EBX-700 EBU-4000QN-CDCX-MC-4DCX-MC-10 DCX4KDCX10K EPS-300XEPX-250X12-13 QNC-700QuadraCoolMiniVertical SlabGelBlottingSystem 10x101-4yes0.751.01.5101416coolinglabryinthBuiltinEBU-204 EBX-700 EBU-4000QN-CDCX-MC-4DCX-MC-10 DCX4KDCX10K EPS-300XEPX-250X12-13 MGV-102VerticalMiniGelKitSingle11.3x101yes0.50.751.01.52.013E2m7101418noEBU-204EBX-700EBU-4000StandardwithkitGCC-204GCC-204K GCC-210GCC-210K EPS-300X14-15 MGV-202VerticalMiniGelKitDual11.3x101-2yes0.50.751.01.52.013E2m7101418internalcooling chamberexternal chillerrequired EBU-204EBX-700EBU-4000StandardwithkitGCC-204GCC-204K GCC-210GCC-210K EPS-300X14-15 MGV-302VerticalMiniGelKitSingle10x81yes0.50.751.01.52.01101416noEBU-204EBX-700EBU-4000StandardwithkitGCX-204GCX-204K GCX-210GCX-210K EPS-300X14-15 MGV-402VerticalMiniGelKitDual10x81-2yes0.50.751.01.52.01101416internalcooling chamberexternal chillerrequired EBU-204EBX-700EBU-4000StandardwithkitGCX-204GCX-204K GCX-210GCX-210K EPS-300X14-15 WideDualMini-Verticals CatProductGlassPlateSize wxh cm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofWellsTemperatureControl Method CompatibleTank BlottingSystems CompatibleSemi-Dry BlottingSystems GelCastingOptionsRecommendedPowerSupplyCat. PAGEGelWrapMultiGelCaster DCX-800DualCoolMidiVerticalSlab GelBlottingSystem 15x101-2yes0.751.01.51222026noBuiltinEBU-402EBU-3000StandardwithkitDCX8-MC-4EPX-250EPS-300X EPS-600 16-17 DCX-800CDualCoolMidiVerticalSlab GelBlottingSystem 15x101-2yes0.751.01.51222026coolinglabryinthBuiltinEBU-402EBU-3000StandardwithkitDCX8-MC-4EPX-250EPS-300X EPS-600 16-17 DoubleTriple-WideMini-Verticals20cmand33cmwidths CatProductGlassPlateSize wxhcm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofWellsTemperatureControl Method CompatibleTank BlottingSystems CompatibleSemi-Dry BlottingSystems GelCastingOptionsRecommendedPowerSupplyCat. PAGEGelWrapMultiGelCaster MGV-102-20DoubleWideMiniVertical System 20x101 - 0.50.751.01.512141617202832 3552 internalcooling chamberexternal chillerrequired EBU-402EBU-4000StandardwithkitGCC-204-20GCC- 204-20K EPS-300X18-19 MGV-202-20DualDoubleWideMini VerticalSystem 20x101-2 - 0.50.751.01.512141617202832 3552 internalcooling chamberexternal chillerrequired EBU-402EBU-4000StandardwithkitGCC-204-20GCC- 204-20K EPS-300X18-19 MGV-116-20DoubleWideMiniVertical System 20x161 - 0.50.751.01.512141617202832 3552 noEBU-102EBU-4000StandardwithkitGCC-204-2016GCC- 204-2016K EPS-300X18-19 MGV-216-20DualDoubleWideMini VerticalSystem 20x161-2 - 0.50.751.01.512141617202832 3552 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-204-2016GCC- 204-2016K EPS-300X18-19 MGV-102-33TripleWideMiniVertical System 33x101 - 0.751.01.5313442506063102noEBU-302EBU-3000StandardwithkitGCC-204-33GCC- 204-33K EPS-300X20-21 MGV-202-33DualTripleWideMini VerticalSystem 33x101-2 - 0.751.01.5313442506063102internalcooling chamberexternal chillerrequired EBU-302EBU-3000StandardwithkitGCC-204-33GCC- 204-33K EPS-300X20-21 MGV-116-33TripleWideMiniVertical System 33x161 - 0.751.01.5313442506063102noEBU-362EBU-6000StandardwithkitGCC-264-33GCC- 264-33K EPS-300X20-21 MGV-216-33DualTripleWideMini VerticalSystem 33x161-2 - 0.751.01.5313442506063102internalcooling chamberexternal chillerrequired EBU-362EBU-6000StandardwithkitGCC-264-33GCC- 264-33K EPS-300X20-21 C.B.S.ScientificElectrophoresisSelectionGuide-VERTICALS StandardVerticals-16.5cmwideGlassPlates CatProductGlassPlateSizewxh cm ofGelsper Unit PrecastGels Compatible CombSpacer Thicknessmm NumberofwellsTemperature ControlMethod CompatibleTank BlottingSystems Compatible Semi-DryBlotting Systems GelCastingOptionsRecommendedPower Supply Cat. PAGE GelWrapMultiGelCaster ASG-250-02AdjustableVerticalGel SystemCE 16.5x14.5-281 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60026-27 DASG-250DualAdjustableVertical GelSystem 16.5x14.5-281-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60026-27 DASG-250-02DualAdjustableVertical GelSystemCE 16.5x14.5-281-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60026-27 DASG-400DualAdjustableVertical GelSystem 16.5-38.71-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-6000StandardwithkitEPS-300XEPS-600 EPS-3000 26-27 Selection Guide - Verticals w w w. c b s s c i e n t i f i c . c o m6 Multi-channelpipettorformat LargeVerticals-20cmwideglassplates CatProductGlassPlateSizewxh cm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofwellsTemperatureControl Method CompatibleTank BlottingSystems CompatibleSemi- DryBlottingSystems GelCastingOptionsRecommendedPowerSupplyCat. PAGE GelWrapMultiGelCaster LSG-400-20LargeFormatVerticalGel System 20x22-421 - 0.50.751.01.52.012348121416172028 3235 heatdispersionplateEBU-222EBU-422EBU-4000EBU- 6000 StandardwithkitEPS-300XEPS-600 EPS-3000 30-31 LDASG-400-20DualLargeFormatVertical GelSystem 20x22-421-2 - 0.50.751.01.52.012348121416172028 3235 heatdispersionplateEBU-222EBU-422EBU-4000EBU- 6000 StandardwithkitEPS-300XEPS-600 EPS-3000 30-31 DCX-900CCooledLargeFormatGel System 20x221-2 - 0.20.40.50.751.0 1.52.03.06.0 12D23E3m4568101214 1516202930 coolinglabyrinthEBU-222EBU-4000StandardwithkitDCX9-MC-4EPS-250XEPS-300X EPS-600 28-29 ExtraLargeVerticals-50cmwideglassplates CatProductGlassPlateSizewxh cm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofwellsTemperatureControl Method CompatibleTank BlottingSystems CompatibleSemi- DryBlottingSystems GelCastingOptionsRecommendedPowerSupplyCat. PAGE GelWrapMultiGelCaster C-DASG-400-50MegaGelDualHigh ThroughputSystem 50x22-321-2-1.01.550100heatdispersionplateStandardwithkitEPS-60032 LPEVerticals-PoreGradientLipoproteinSystem CatProductGlassPlateSizewxh cm ofGelsperUnitPrecastGels Compatible CombSpacer Thicknessmm NumberofwellsTemperatureControl Method RecommendedPowerSupplyCat. PAGE LPE-4003PoreGradientLipoprotein System 8.2x8.24yes121318CoolingCoilEPS-300X33 DASG-400-02DualAdjustableVertical GelSystemCE 16.5x14.5-38.71-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-6000StandardwithkitEPS-300XEPS-600 EPS-3000 26-27 DSG-125-02DualSlabGelSystem16.5x14.51-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60024-25 DSG-160-02DualSlabGelSystem16.5x161-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60024-25 DSG-170-02DualSlabGelSystem16.5x171-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60024-25 DSG-190-02DualSlabGelSystem16.5x191-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60024-25 DSG-200-02DualSlabGelSystem16.5x221-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60024-25 DSG-250-02DualSlabGelSystem16.5x281-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-6000StandardwithkitEPS-300XEPS-60024-25 DSG-400DualSlabGelSystem16.5x38.71-2 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 internalcooling chamberexternal chillerrequired EBU-102EBU-6000StandardwithkitEPS-300XEPS-60024-25 SG-125C.B.S.LiteVertical System 16.5x14.51 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-125-02C.B.S.LiteVertical SystemCE 16.5x14.51 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-160C.B.S.LiteVertical System 16.5x161 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-160-02C.B.S.LiteVertical SystemCE 16.5x161 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-170C.B.S.LiteVertical System 16.5x171 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-170-02C.B.S.LiteVertical SystemCE 16.5x171 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-190C.B.S.LiteVertical System 16.5x191 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-190-02C.B.S.LiteVertical SystemCE 16.5x191 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-200C.B.S.LiteVertical System 16.5x221 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-200-02C.B.S.LiteVertical SystemCE 16.5x221 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-4000StandardwithkitGCC-404GCC-404K GCC-410GCC-410K EPS-300XEPS-60022-23 SG-250C.B.S.LiteVertical System 16.5x281 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-6000StandardwithkitEPS-300XEPS-60022-23 SG-250-02C.B.S.LiteVertical SystemCE 16.5x281 - 0.20.40.50.751.01.52.0 3.06.0 123E3m456810 12141516202930 heatdispersion plate EBU-102EBU-6000StandardwithkitEPS-300XEPS-60022-23 p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 7 Mini-Horizontals CatProductOverallUnit lxwxh cm GelTraySize wxl cm Numberof Slotsingel tray Maximum Numberof Samples pergel NumberofwellsComb Thickness mm Minimumbuffer volumeml Buffer Recirculation capability Tempera- ture Control Capability CastingOptionsGelTray options Recommended PowerSupply Cat. PAGE InsituExternalGelcasterAdjustableGelCaster MHU-102HorizontalMiniGelKit18.2x8x95.5x8.523213E3M81012 1416 1.02.0140Availableupon request noInsituGCT-1011-place GCT-1033-place GCT-1066-place AGCT-16520MGT-122EPS-300X42-43 MHU-102-FLHorizontalMiniGelKit FlipLid 18.2x8x95.5x8.523213E3M81012 1416 1.02.0140Availableupon request noInsituGCT-1011-place GCT-1033-place GCT-1066-place AGCT-16520MGT-122EPS-300X42-43 TLK-GCMHU-102TeachingLabMiniGel Bundle 18.2x8x95.5x8.523213E3M81012 1416 1.02.0140Availableupon request noInsituGCT-1011-place GCT-1033-place GCT-1066-place AGCT-16520MGT-122EPS-300XCall for info. GCMHU-102HorizontalMiniGel ProductivityPackage 18.2x8x95.5x8.523213E3M81012 1416 1.02.0140Availableupon request noInsituGCT-1011-place GCT-1033-place GCT-1066-place AGCT-16520MGT-122EPS-300X42-43 MHU-202HorizontalMiniGelKit20x9.7x107.5x1023213E3M5810 121416 1.02.0235Availableupon request noInsituGCT-2011-place GCT-2033-place GCT-2066-place AGCT-16520MGT-222EPS-300X42-43 MHU-202-FLHorizontalMiniGelKit FlipLid 20x9.7x107.5x1023213E3M5810 121416 1.02.0235Availableupon request noInsituGCT-2011-place GCT-2033-place GCT-2066-place AGCT-16520MGT-222EPS-300X42-43 TLK-GCMHU-202TeachingLabMiniGel Bundle 20x9.7x107.5x1023213E3M5810 121416 1.02.0235Availableupon request noInsituGCT-2011-place GCT-2033-place GCT-2066-place AGCT-16520MGT-222EPS-300XCall for info. GCMHU-202HorizontalMiniGel ProductivityPackage 20x9.7x107.5x1023213E3M5810 121416 1.02.0235Availableupon request noInsituGCT-2011-place GCT-2033-place GCT-2066-place AGCT-16520MGT-222EPS-300X42-43 MGU-252THighVisibilityMiniGelKit20x9.7x107.5x1023213E3M5810 121416 1.02.0235Availableupon request noInsituGCT-2011-place GCT-2033-place GCT-2066-place AGCT-16520MGT-222EPS-300X41 MGU-303Mini-FastSub17x14x810x82404816201.02.050notavailablenoInsitu___EPS-300X40 Midi-Horizontals CatProductOverallUnit lxwxh cm GelTraySize wxl cm Numberof Slotsingel tray Maximum Numberof Samples pergel NumberofwellsComb Thickness mm Minimumbuffer volumeml BufferRecircu- lationCapability Tempera- tureControl Capability CastingOptionsGelTray options Recommended PowerSupply Cat. PAGE InsituExternalGelcasterAdjustableGelCaster MHU-402HorizontalMidiGelKit22.5x13x1410.5x1123651012181.01.5550Availableupon request noInsituGCT-10-1111-place GCT-10-1122-place AGCT-16520AGCT-21826 AGCT-27940 MGT-400EPS-300X44-45 MHU-402-FLHorizontalMidiGelKit FlipLid 22.5x13x1410.5x1123651012181.01.5550Availableupon request noInsituGCT-10-1111-place GCT-10-1122-place AGCT-16520AGCT-21826 AGCT-27940 MGT-400EPS-300X44-45 GCMHU-402HorizontalMidiGel ProductivityPackage 22.5x13x1410.5x1123651012181.01.5550Availableupon request noInsituGCT-10-1111-place GCT-10-1122-place AGCT-16520AGCT-21826 AGCT-27940 MGT-400EPS-300X44-45 MHU-502HorizontalMidiGelKit21.5x16.7x1414x1025413E3M5810 12131620 2627 1.01.52.0 3.0 700Availableupon request noInsituGCT-14-1011-place GCT-14-1022-place AGCT-16520AGCT-21826 AGCT-27940 MGT-500EPS-300X44-45 MHU-502-FLHorizontalMidiGelKit FlipLid 21.5x16.7x1414x1025413E3M5810 12131620 2627 1.01.52.0 3.0 700Availableupon request noInsituGCT-14-1011-place GCT-14-1022-place GCT-14-101GCT-14-102 AGCT-16520 MGT-500EPS-300X44-45 GCMHU-502HorizontalMidiGel ProductivityPackage 21.5x16.7x1414x1025413E3M5810 12131620 2627 1.01.52.0 3.0 700Availableupon request noInsituGCT-14-1011-place GCT-14-1022-place AGCT-16520AGCT-21826 AGCT-27940 MGT-500EPS-300X44-45 MHU-602HorizontalMidiGelKit27.5x16.7x1414x16410813E3M5810 12131620 2627 1.01.52.0 3.0 750Availableupon request noInsituGCT-14-1611-place GCT-14-1622-place AGCT-16520AGCT-21826 AGCT-27940 MGT-600EPS-300X44-45 MHU-602-FLHorizontalMidiGelKit FlipLid 27.5x16.7x1414x16410813E3M5810 12131620 2627 1.01.52.0 3.0 750Availableupon request noInsituGCT-14-1611-place GCT-14-1622-place AGCT-16520AGCT-21826 AGCT-27940 MGT-600EPS-300X44-45 GCMHU-602HorizontalMidiGel ProductivityPackage 27.5x16.7x1414x16410813E3M5810 12131620 2627 1.01.52.0 3.0 750Availableupon request noInsituGCT-14-1611-place GCT-14-1622-place AGCT-16520AGCT-21826 AGCT-27940 MGT-600EPS-300X44-45 WMGE-600FlexicastHorizontal System3in1 27.5x16.7x1414x1614x12 14x8 14x164 14x123 14x82 14x16108 14x1281 14x854 13E3M5810 12131620 2627 1.01.52.0 3.0 750Availableupon request no_AGCT-16520included AGCT-21826AGCT-27940 WGT-1408 WGT-1412 WGT-1416 EPS-300X46-47 C.B.S.ScientificElectrophoresisSelectionGuide-HORIZONTALS Selection Guide - Horizontals w w w. c b s s c i e n t i f i c . c o m8 Selection Guide - Horizontals StandardHorizontals CatProductOverallUnit lxwxh cm GelTraySize wxl cm Numberof Slotsingel tray Maximum Numberof Samples pergel NumberofwellsCombThick- nessmm Minimumbuffer volumeml bufferrecircula- tioncapability Tempera- ture Control Capability CastingOptionsGelTrayoptionsRecommended PowerSupply Cat. PAGE InsituExternalGelCasterAdjustableGelCaster GCHSE-014Econo-SubHorizontal ProductivityPackage 38x15x1014x20616213E3M5810 12131620 2627 1.01.52.0 3.0 1500YesYes_GCT-14-2011-place GCT-14-2022-place AGCT-16520AGCT-21826 AGCT-27940 WGT-014EPS-300X50-51 GCHSE-020Econo-SubHorizontal ProductivityPackage 43x21x1020x20624013E3M5812 16203040 1.01.52.0 3.0 1850YesYes_GCT-20-2011-place GCT-20-2022-place GCT-20-201GCT-20-202 AGCT-21826AGCT-27940 WGT-020EPS-300X50-51 GCHSE-030Econo-SubHorizontal ProductivityPackage 53x21x1020x30936013E3M5812 16203040 1.01.52.0 3.0 1950YesYes_GCT-20-3011-place GCT-20-3022-place GCT-20-301GCT-20-302 AGCT-27940 WGT-030EPS-300X50-51 GCHSE-040Econo-SubHorizontal ProductivityPackage 63x21x1020x401248013E3M5812 16203040 1.01.5 2.03.0 2050YesYes_GCT-20-4011-place GCT-20-4022-place AGCT-27940WGT-040EPS-300X50-51 HSU-014SubmarineHorizonal GelKit 38x15x1014x20616213E3M58 10121316 202627 1.01.5 2.03.0 1500YesYesInsituGCT-14-2011-place GCT-14-2022-place GCT-14-201GCT-14-202 AGCT-16520AGCT- 21826AGCT-27940 WGT-014EPS-300X48-49 GCHSU-014SubmarineHorizonal GelProductivityPack- age 38x15x1014x20616213E3M58 10121316 202627 1.01.5 2.03.0 1500YesYesInsituGCT-14-2011-place GCT-14-2022-place AGCT-16520 AGCT-21826 AGCT-27940 WGT-014EPS-300X48-49 HSU-020SubmarineHorizonal GelKit 43x21x1020x20624013E3M5 8121620 3040 1.01.5 2.03.0 1850YesYesInsituGCT-20-2011-place GCT-20-2022-place AGCT-21826 AGCT-27940 WGT-020EPS-300X48-49 GCHSU-020SubmarineHorizonal GelProductivityPack- age 43x21x1020x20624013E3M5 8121620 3040 1.01.5 2.03.0 1850YesYesInsituGCT-20-2011-place GCT-20-2022-place AGCT-21826 AGCT-27940 WGT-020EPS-300X48-49 HSU-030SubmarineHorizonal GelKit 53x21x1020x30936013E3M5 8121620 3040 1.01.5 2.03.0 1950YesYesInsituGCT-20-3011-place GCT-20-3022-place AGCT-27940WGT-030EPS-300X48-49 GCHSU-030SubmarineHorizonal GelProductivityPack- age 53x21x1020x30936013E3M5 8121620 3040 1.01.5 2.03.0 1950YesYesInsituGCT-20-3011-place GCT-20-3022-place AGCT-27940WGT-030EPS-300X48-49 HSU-040SubmarineHorizonal GelKit 63x21x1020x401248013E3M5 8121620 3040 1.01.5 2.03.0 2050YesYesInsituGCT-14-2011-place GCT-14-2022-place AGCT-27940WGT-040EPS-300X EPS-600 48-49 GCHSU-040SubmarineHorizonal GelProductivityPack- age 63x21x1020x401248013E3M5 8121620 3040 1.01.5 2.03.0 2050YesYesInsituGCT-14-2011-place GCT-14-2022-place AGCT-27940WGT-040EPS-300X EPS-600 48-49 WHSE-014FlexicastHorizontal System4in1 38x15x1014x20616213E3M58 10121316 202627 1.01.5 2.03.0 1600YesYes_AGCT-16520included AGCT-21826 AGCT-27940 WGT-1420 WGT-1416 WGT-1412 WGT-1408 EPS-300X46-47 14x164108 14x12381 14x8254 High-ThroughputHorizontals CatProductOverallUnit lxwxh cm GelTraySize wxl cm Numberof Slotsingel tray Maximum Numberof Samples pergel NumberofwellsCombThick- nessmm Minimumbuffer volume ml Bufferrecircula- tioncapability Tempera- tureControl Capability CastingOptionsGelTrayoptionsRecommended PowerSupply Cat. PAGE InsituExternalGelCasterAdjustableGelCaster HSU-2614HighThroughput HorizontalSystem 38x28x1026x14552525-1MA25-1MB 2627-3M28-4M 51102 0.751.0 1.52.0 2200YesYesInsituGCT-26-1411-place GCT-26-1422-place AGCT-27940WGT-2614EPS-300X52-53 HSU-2626HighThroughput HorizontalSystem 48x28x1026x26881625-1MA25-1MB 2627-3M28-4M 51102 0.751.0 1.52.0 2600YesYesInsituGCT-26-2611-place GCT-26-2622-place GCT-26-261GCT-26-262 AGCT-27940 WGT-2626EPS-300X52-53 HSU-2640HighThroughput HorizontalSystem 64x28x1026x40881625-1MA25-1MB 2627-3M28-4M 51102 0.751.0 1.52.0 3400YesYesInsituGCT-26-4011-place GCT-26-4022-place AGCT-27940WGT-2640EPS-300X EPS-600 52-53 Multi-channelpipettorformat p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 9 w w w. c b s s c i e n t i f i c . c o m10 VERTICALS FEATURES Dual capacity with dual function - run either 2 slab gels or 2 Western blots as shown in figure 1. Each gel or blotting cassette is placed into a double-gasketed receptacle in the core for increased perimeter pressure ensuring a tight fit and preventing surrounding buffer interference. Hinged core design allows easy loading and removal of gel or blotting cassettes. Dual Cool System cat. DCX-700 will accept 10cmw x 10cml 10cmwx 9cml or 10cmw x 8cml precast gels. Alternatively handcasting can be accomplished using Gel Wrap Casting Bundle and Multi-Caster Kits. Gel or blotting cassettes can be extracted from the core without removing the core from the tank allowing for quick processing of samples. This feature also allows for the immediate reuse of buffer for the next set of samples. Provides the capability of running 2 gels or 2 blots simultaneously under identical temperature controlled buffer conditions using freezer blocks. Color-coded anode and cathode safety features on all components. Tank design includes stirring bar corral to maintain even temperature. There are no loose parts to keep track of on the central core. Electrophoretic transfers do NOT require the purchase of special transfer modules. DCX-700 series runs or blots two mini-gels NuPAGE is a trademark of Invitrogen Corporation PAGEr is a trademark of Lonza Expedeon is a trademark of Expedeon and SERVAGel is a trademark of Serva. ACTGene DGel is a trademark of ACTGene Bio-Rad is a trademark of Bio-Rad Laboratories Inc. Dual Cool Mini-Vertical Dual Cool System DCX-700 Complete System Lower buffer reservoir Dual capacity core Platinum electrodes 2 freezer blocks 2 blotting cassettes with sponge pads Safety cover with power leads Conversion plate for running one gel at a time figure 1. ClearPAGE SDS gels- TEO packages of 10 - Cat. s Acrylamide Gel Size cm 12 well 17 well 8 10 x10 FK00812-10 FK00817-10 10 10 x10 FK01012-10 FK01017-10 12 10 x10 FK01212-10 FK01217-10 16 10 x10 FK01612-10 FK01617-10 4 - 8 10 x10 FK40812-10 FK40817-10 4 - 12 10 x10 FK41212-10 FK41217-10 4 - 20 10 x10 FK42012-10 FK42017-10 Precast Gels Superior quality SDS PAGE and DNANative PAGE precast gels are described in detail on pages 58-61. Two sizes can be run on the Dual Cool Mini-Vertical 10cmw x 10cmh or 10cmw x 8cm h. Choose 10cm x 10cm gel catalog number from chart below. Alternatively choose 10cm x 8cm ClearPAGE gels on page 59 or one of the other compatible precast gels listed above. PAGE Precast Gels Accessories APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Western Blot SPECIFICATIONS Number of gels 1-2 Compatible Precast gels NuPAGE PAGEr Expedeon SERVAGel ACTGene DGel Bio-Rad TGX Compatible Precast Gel Sizes cm 10w x 10l 10w x 9l 10w x 8l Handcast Glass Plate Set Dimensions cm 10w x 10l Cathode Buffer Volume for 2 Gels 200mls Anode Buffer Volume 400mls minimum - 800mls Total Buffer Volume for 2 Blots 940mls Slab Gel Run Time 30-90 minutes Blot Run Time 75 minutes Recommended Power Supply EPS-300 X or EPX-250 X Overall Dimensions W x D x H cm 16.5 x 15.2 x 21.6 PAGE Precast Gels Accessories Bio-Rad TGX gels require shim adapter cat. DCX-SP-108 p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 11 VERTICALS 2 Handcast Gel Options for Dual Cool Systems ORDERING INFORMATION CAT. DESCRIPTION DUAL COOL MINI-VERTICALS - 10CMW X 10CMH DCX-700 Dual Cool Mini-Vertical CE 2-place Complete System Accessories and Related Products for DCX-700 EBX-BC-700 Accessory Blotting Cassette includes sponge pad EBX-SP-700 Accessory Sponge Pads set of 4 DCX-SP-108 Accessory shim plate for 8cmw Bio-Rad gels DCX-P1010R Gel Wrap Glass Plate Set DCX-E0710 Gel Wrap Gasket 0.75mm thick DCX-E1010 Gel Wrap Gasket 1.0mm thick DCX-E1510 Gel Wrap Gasket 1.5mm thick DCX-S0710R Gel Wrap Spacer Set 0.75mm thick DCX-S1010R Gel Wrap Spacer Set 1.0mm thick DCX-S1510R Gel Wrap Spacer Set 1.5mm thick DCX-MC-4 Mini-Vertical Multi-Caster 4-place Unit Only DCX-P1010 Multi-Caster Glass Plate Set DCX-S0710 Multi-Caster Spacer Set 0.75mm thick DCX-S1010 Multi-Caster Spacer Set 1.0mm thick DCX-S1510 Multi-Caster Spacer Set 1.5mm thick EBU-204 Mini-Blotting System CE 4 place fits 10 x 10cm gels EBX-700 Mini-Blotting System CE 4 place fits 10 x 10cm gels EBU-4000 Semi-Dry-Blotting System CE fits four 10 x 10cm gels EPS-300 X Power Supply CE 1-300V 1-500mA The Gel Wrap Casting System Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. Online video instructions can be found at httpwww.cbsscientific.cominstructionvideos.aspx. 5 Seconds 15 Seconds 25 Seconds 45 Seconds 60 Seconds Disassembly Blotting Systems Tank Mini-Blotting Systems EBU-204 or EBX-700 fit four 10cm x 10cm gels and are for the lab that needs a dedicated blotting system. See pages 34- 35. Semi-Dry Blotting System EBU-4000 fits four 10 x 10 gels at a time and is described on page 39. Online video instructions for both of these methods can be found at httpwww.cbsscientific.cominstructionvideos.aspx. COMB OPTIONS material PTFE tooth depth 9.5mm overall length 7.9cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MVX-0701 1 0.75 66.6 356 MVX-0710 10 0.75 5.23 28 MVX-0714 14 0.75 3.3 19 MVX-0716 16 0.75 2.92 16 MVX-1001 1 1.0 66.6 474 MVX-1010 10 1.0 5.23 37 MVX-1014 14 1.0 3.3 24 MVX-1016 16 1.0 2.92 21 MVX-1501 1 1.5 66.6 712 MVX-1510 10 1.5 5.23 56 MVX-1514 14 1.5 3.3 35 MVX-1516 16 1.5 2.92 31 Maximum loading volume is calculated by taking 75 of the total tooth volume. OPTION 1 Multi-Caster Method 4-Place Multi-Caster Kit Ordering Information - Cat. s Comb Wells Spacer Comb Thickness 0.75mm Spacer Comb Thickness 1.0mm Spacer Comb Thickness 1.5mm 10 DCX4K-0710 DCX4K-1010 DCX4K-1510 14 DCX4K-0714 DCX4K-1014 DCX4K-1514 16 DCX4K-0716 DCX4K-1016 DCX4K-1516 OPTION 2 Gel Wrap Casting System see description above right DCX4K Multi-Caster Kit 4-place Complete System Multi-Caster Chamber with lid silicone gasket 5 stainless steel thumbscrews backfill port connecting to central filling trough See page 23 for assembly diagram 4 sets notched glass plate sets 4 sets of spacers choice of 0.75mm 1.0mm or 1.5mm thickness 4 combs choice of 10 14 or 16 well Polycarbonate separation sheets Acrylic shim plate DCX-HC Dual Cool Gel Wrap Casting Bundle 2-place Complete System DCX-700 Dual Cool Mini-Vertical 2 Gel Wrap Gaskets 2 sets of Gel Wrap glass plates 2 sets of Gel Wrap spacers 2 combs 6 clamps Dual Cool Gel Wrap Casting Bundle 2-Place - Cat. s Comb Wells Spacer Comb Thickness 0.75mm Spacer Comb Thickness 1.0mm Spacer Comb Thickness 1.5mm 10 DCX-HC0710 DCX-HC1010 DCX-HC1510 14 DCX-HC0714 DCX-HC1014 DCX-HC1514 16 DCX-HC0716 DCX-HC1016 DCX-HC1016 w w w. c b s s c i e n t i f i c . c o m12 VERTICALS Quadra Mini-Verticals Quadra Mini-Vertical Cooled QNC-700 Complete System Lower buffer reservoir with double-sided cooling labyrinth and tubing adapters shown with optional self-closing quick- disconnect valves 2 dual-capacity cores Platinum electrodes 4 blotting cassettes with sponge pads Safety cover with power leads Conversion plate for running one gel at a time Quadra Mini-Vertical QNX-700 Complete System Lower buffer reservoir 2 dual-capacity cores Platinum electrodes 4 blotting cassettes with sponge pads Safety cover with power leads Conversion plate for running one gel at a time Quadra series runs or blots four mini-gels quadruple capacityquadruple capacityquadruple capacity with dual functionwith dual functionwith dual function PAGE Precast Gels Accessories Bio-Rad TGX gels require shim adapter cat. DCX-SP-108 FEATURES Each gel or blotting cassette is placed into a double-gasketed receptacle in the core for increased perimeter pressure ensuring a tight fit and preventing surrounding buffer interference. Hinged core design allows easy loading and removal of gel or blotting cassettes. Quadras will accept 10cmw x 10cml 10cmwx 9cml or 10cmw x 8cml precast gels. Alternatively handcasting can be accomplished using Gel Wrap Casting Bundle and Multi-Caster Kits. See next page for kit descriptions and ordering information. Gel or blotting cassettes can be extracted from the core without removing the core from the tank allowing for quick processing of samples. This feature also allows for the immediate reuse of buffer for the next set of samples. The Cooled Quadra cat. QNC-700 provides the capability of running 4 gels or 4 blots simultaneously under identical temperature controlled buffer conditions. This unit has double-sided cooling labyrinths and tubing adapters. Optional self-closing quick-disconnect valves cat. QDC-P shown above simplify connection to an external cooler. Color-coded anode and cathode safety features on all components. Tank design includes stirring bar corral to maintain even temperature. There are no loose parts to keep track of on the central core. Electrophoretic transfers do NOT require the purchase of special transfer modules. SPECIFICATIONS Number of gels 1-4 Compatible Precast gels NuPAGE PAGEr Expedeon SERVAGel ACTGene DGel Bio-Rad TGX gels Compatible Precast Gel Sizes cm 10w x 10l 10w x 9l 10w x 8l Handcast Glass Plate Dimensions cm 10w x 10l Cathode Buffer Volume for 4 Slab Gels 200mlsreservoir total of 400mls Anode Buffer Volume 950-1100 mls Total Buffer Volume for 4 Blots 1700mls Slab Gel Run Time 30-90 minutes Blot Run Time 75 minutes Recommended Power Supply EPS-300 X or EPX-250 X Overall Dimensions W x D x H cm 17.8 x 17.1 x 15.2 APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Western Blot p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 13 VERTICALS ORDERING INFORMATION CAT. DESCRIPTION QUADRA COMBO MINI-VERTICALS - 10CMW X 10CMH QNX-700 Quadra System CE 4-place Complete System QNC-700 Quadra System CE 4-place Cooled Complete System Accessories and Related Products for QNX-700 QNC-700 EBX-BC-700 Accessory Blotting Cassette includes sponge pad EBX-SP-700 Accessory Sponge Pads set of 4 QDC-P Quick-Disconnect Coupling self-closing DCX-SP-108 Accessory shim plate for 8cmw Bio-Rad gels DCX-P1010R Gel Wrap Glass Plate Set DCX-E0710 Gel Wrap Gasket 0.75mm thick DCX-E1010 Gel Wrap Gasket 1.0mm thick DCX-E1510 Gel Wrap Gasket 1.5mm thick DCX-S0710R Gel Wrap Spacer Set 0.75mm thick DCX-S1010R Gel Wrap Spacer Set 1.0mm thick DCX-S1510R Gel Wrap Spacer Set 1.5mm thick DCX-MC-4 Mini-Vertical Multi-Caster 4-place Unit Only DCX-P1010 Multi-Caster Glass Plate Set DCX-S0710 Multi-Caster Spacer Set 0.75mm thick DCX-S1010 Multi-Caster Spacer Set 1.0mm thick DCX-S1510 Multi-Caster Spacer Set 1.5mm thick EBU-204 Mini-Blotting System CE 4 place fits 10 x 10cm gels EBX-700 Mini-Blotting System CE 4 place fits 10 x 10cm gels EBU-4000 Semi-Dry Blotting System transfer area of 20cm x 20cm EPS-300 X Power Supply CE 1-300V 1-500mA EPX-250 X High Current Power Supply CE 2-250V 10-2500mA Blotting Systems Precast Gel Options for Quadra Systems Precast Gels Superior quality SDS PAGE and DNANative PAGE precast gels. Two sizes available for these units 10cmw x 10cml or 10cmw x 8cm l. For more information see pages 58-61. PAGE Precast Gels Accessories ClearPAGE SDS gels- TEO packages of 10 - Cat. s Acrylamide Gel Size cm 12 well 17 well 8 10 x10 FK00812-10 FK00817-10 10 10 x10 FK01012-10 FK01017-10 12 10 x10 FK01212-10 FK01217-10 16 10 x10 FK01612-10 FK01617-10 4 - 8 10 x10 FK40812-10 FK40817-10 4 - 12 10 x10 FK41212-10 FK41217-10 4 - 20 10 x10 FK42012-10 FK42017-10 OPTION 1 Multi-Caster Method 2 Handcast Gel Options for Quadra Systems 4-Place Multi-Caster Kit Ordering Information - Cat. s Comb Wells Spacer Comb Thickness 0.75mm Spacer Comb Thickness 1.0mm Spacer Comb Thickness 1.5mm 10 DCX4K-0710 DCX4K-1010 DCX4K-1510 14 DCX4K-0714 DCX4K-1014 DCX4K-1514 16 DCX4K-0716 DCX4K-1016 DCX4K-1516 Quadra Gel Wrap Casting Bundle 4-Place - Cat. s Comb Wells Spacer Comb Thickness 0.75mm Spacer Comb Thickness 1.0mm Spacer Comb Thickness 1.5mm 10 QN-C0710 QN-C1010 QN-C1510 14 QN-C0714 QN-C1014 QN-C1514 16 QN-C0716 QN-C1016 QN-C1516 OPTION 2 Gel Wrap Casting System see description on page 11 Tank Mini-Blotting Systems EBU-204 or EBX-700 fit four 10cm x 10cm gels and are for the lab that needs a dedicated blotting system. See pages 34-35. Semi-Blotting System EBU-4000 shown at left has a transfer area of 20cm x 20cm allowing the transfer of four 10cm x 10cm simultaneously. See page 39. COMB OPTIONS material PTFE tooth depth 9.5mm overall length 7.9cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MVX-0701 1 0.75 66.6 356 MVX-0710 10 0.75 5.23 28 MVX-0714 14 0.75 3.3 19 MVX-0716 16 0.75 2.92 16 MVX-1001 1 1.0 66.6 474 MVX-1010 10 1.0 5.23 37 MVX-1014 14 1.0 3.3 24 MVX-1016 16 1.0 2.92 21 MVX-1501 1 1.5 66.6 712 MVX-1510 10 1.5 5.23 56 MVX-1514 14 1.5 3.3 35 MVX-1516 16 1.5 2.92 31 Maximum loading volume is calculated by taking 75 of the total tooth volume. DCX4K Multi-Caster Kit 4-place Complete System DCX4K Multi-Caster Chamber with lid silicone gasket 5 stainless steel thumbscrews backfill port connecting to central filling trough See page 23 for assembly diagram 4 sets notched glass plate sets 4 sets of spacers choice of 0.75mm 1.0mm or 1.5mm thickness 4 combs choice of 10 14 or 16 well Polycarbonate separation sheets Acrylic shim plate QN-C Quadra Gel Wrap Casting Bundle 4-place Complete System 4 Gel Wrap Gaskets 4 sets of Gel Wrap glass plates 4 sets of Gel Wrap spacers 4 combs 12 clamps w w w. c b s s c i e n t i f i c . c o m14 VERTICALS FEATURES All MGV Mini-Vertical Systems will accept ClearPAGE precast gels. The MGV-302 and MGV-402 fit 10 x 8cm ClearPAGE gels while the MGV-102 and MGV-202 fit the 10cm x 10cm ClearPAGE precast gels. The internal cooling chamber of Dual units provides the capability of running 2 gels simultaneously under identical temperature controlled buffer conditions Compatible Blotting Systems run up to 4 gels at a time Choice of 2 methods for handcasting of gels Small size permits rapid analysis of samples and reduces use of expensive reagents needed for gel formation and running buffer. A wide assortment of accessories are available for all sizes. 2 sizes of Mini-Verticals fit precast or handcast gels Mini-Verticals Dual and Single Single Mini-Vertical MGV-102 and MGV-302 Complete System Electrophoresis Unit Silicone gaskets on front of unit Platinum electrodes Safety cover with power leads 1 comb 1 sets of Gel Wrap glass plates 1 sets of Gel Wrap spacers 1 Gel Wrap gasket 5 spring clamps Dual Mini-Vertical MGV-202 and MGV-402 Complete System Electrophoresis Unit with internal cooling chamber and coolant recirculation ports Silicone gaskets on both sides of unit Platinum electrodes Safety cover with power leads 2 combs 2 sets of Gel Wrap glass plates 2 sets of Gel Wrap spacers 2 Gel Wrap gaskets 10 spring clamps Conversion plate for running one gel at a time SPECIFICATIONS Cat. Single or Dual Gel Capacity Buffer Volumes ml Overall Dimensions W x D x H cm Glass plate Cassette size W x H cm Precast Gels W x H cm Run Time Multiple Gel Casting Chamber Blotting Chamber MGV-302 single 1 110 14 x 18 x 10 10 x 8 ClearPAGE 10 x 8 25-75 minutes GCX-204 GCX-204K EBU-204 EBX-700 EBU-4000 MGV-402 dual 1-2 205 15 x 18 x 10 MGV-102 single 1 155 14 x 19.7 x 12.7 11.3 x 10 10 x 10 ClearPAGE 10 x 10 30 -90 minutes GCC-204 GCC-204K EBU-204 EBX-700 EBU-4000 MGV-202 dual 1-2 250 17 x 19.7 x 12.7 Blotting Systems APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Multi-Gel Casting Chamber Kit includes Chamber separation sheets 4 sets of glass plates 4 sets of spacers and 4 combs Semi-Dry Blotter EBU-4000 fits 4 gels with dimensions up to 10cm x 10cm. See page 39 for more information. Tank Mini-Blotting Systems EBU-204 or EBX-700 fit gel dimensions up to 10cm x 10cm gels. Compatible for gels used with any of the MGV units. See pages 34-35. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 15 VERTICALS OPTION 1 Multi-Caster Method 2 Handcast Gel Options for Mini-Vertical Systems OPTION 2 Gel Wrap Casting System COMB OPTIONS for 11.3cm wide units MGV-102 MGV-202 material PTFE tooth depth 9.5mm overall length 9.9cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MVC-0707 7 0.75 9.2 49 MVC-0710 10 0.75 5.8 31 MVC-0714 14 0.75 3.4 18 MVC-0718 18 0.75 2.9 11 MVC-1007 7 1.0 9.2 65 MVC-1010 10 1.0 5.8 41 MVC-1014 14 1.0 3.4 25 MVC-1018 18 1.0 2.9 15 MVC-1507 7 1.5 9.2 120 MVC-1510 10 1.5 5.8 61 MVC-1514 14 1.5 3.4 37 MVC-1518 18 1.5 2.9 23 Precast Gel Options for Mini-Vertical Systems Precast Gels Superior quality SDS PAGE and DNANative PAGE precast gels. Available in two sizes 10cmw x 10cmh or 10cmw x 8cm h. See pages 58-61 for more information. PAGE Precast Gels Accessories Multi-Caster 4-place GCX-204 GCC-204 Complete System Multi-Caster Chamber with lid silicone gasket 5 stainless steel thumbscrews backfill port connecting to central filling trough See page 23 for assembly diagram Polycarbonate separation sheets Acrylic shim plate ClearPAGE SDS gels- TEO packages of 10 - Cat. s Acrylamide Fits MGV- Gel Size cm W x H 12 well 17 well 8 302 402 10 x 8 BK00812-10 BK00817-10 102 202 10 x 10 FK00812-10 FK00817-10 10 302 402 10 x 8 BK01012-10 BK01017-10 102 202 10 x 10 FK01012-10 FK01017-10 12 302 402 10 x 8 BK01212-10 BK01217-10 102 202 10 x 10 FK01212-10 FK01217-10 16 302 402 10 x 8 BK01612-10 BK01617-10 102 202 10 x 10 FK01612-10 FK01617-10 4 - 8 302 402 10 x 8 BK40812-10 BK40817-10 102 202 10 x 10 FK40812-10 FK40817-10 4 - 12 302 402 10 x 8 BK41212-10 BK41217-10 102 202 10 x 10 FK41212-10 FK41217-10 4 - 20 302 402 10 x 8 BK42012-10 BK42017-10 102 202 10 x 10 FK42012-10 FK42017-10 ORDERING INFORMATION CAT. DESCRIPTION MINI-VERTICALS - 10CMW X 8CMH MGV-302 Mini-Vertical System Single CE Complete System MGV-402 Mini-Vertical System Dual CE Complete System Accessories and Related Products for MGV-302 MGV-402 MGP-300R Gel Wrap Glass Plate Set MVE-0708 Gel Wrap Gasket 0.75mm thick MVE-1008 Gel Wrap Gasket 1.0mm thick MVE-1508 Gel Wrap Gasket 1.5mm thick MVS-0708R Gel Wrap Spacer Set 0.75mm thick MVS-1008R Gel Wrap Spacer Set 1.0mm thick MVS-1508R Gel Wrap Spacer Set 1.5mm thick GCX-204 Multi-Caster 4-place Unit Only 10cmw MGP-300 Multi-Caster Glass Plate Set 10cm x 8cm MVS-0708 Multi-Caster Spacer Set 0.75mm thick MVS-1008 Multi-Caster Spacer Set 1.0mm thick MVS-1508 Multi-Caster Spacer Set 1.5mm thick MINI-VERTICALS - 11.3CMW X 10CMH MGV-102 Mini-Vertical System Single CE MGV-202 Mini-Vertical System Dual CE Accessories and Related Products for MGV-102 MGV-102 MGP-100R Gel Wrap Glass Plate Set MVE-0710 Gel Wrap Gasket 0.75mm thick MVE-1010 Gel Wrap Gasket 1.0mm thick MVE-1510 Gel Wrap Gasket 1.5mm thick MVS-0710R Gel Wrap Spacer Set 0.75mm thick MVS-1010R Gel Wrap Spacer Set 1.0mm thick MVS-1510R Gel Wrap Spacer Set 1.5mm thick GCC-204 Multi-Caster 4-place Unit Only 11.3cmw MGP-100 Multi-Caster Glass Plate Set MVS-0710 Multi-Caster Spacer Set 0.75mm thick MVS-1010 Multi-Caster Spacer Set 1.0mm thick MVS-1510 Multi-Caster Spacer Set 1.5mm thick EPS-300 X Power Supply CE 1-300V 1-500mA C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Single Systems come with components to handcast 1 gel while the Dual Systems include components to cast 2 gels at a time. See top right of page 11 for series of photos detailing Gel Wrap System. See Product list or website for complete listing of catalog numbers and ordering options Glass plates combs and spacers must be ordered separately. COMB OPTIONS for 10cm wide units MGV-302 MGV-402 material PTFE tooth depth 9.5mm overall length 7.9cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MVX-0701 1 0.75 66.6 356 MVX-0710 10 0.75 5.23 28 MVX-0714 14 0.75 3.3 19 MVX-0716 16 0.75 2.92 16 MVX-1001 1 1.0 66.6 474 MVX-1010 10 1.0 5.23 37 MVX-1014 14 1.0 3.3 24 MVX-1016 16 1.0 2.92 21 MVX-1501 1 1.5 66.6 712 MVX-1510 10 1.5 5.23 56 MVX-1514 14 1.5 3.3 35 MVX-1516 16 1.5 2.92 31 Maximum loading volume is calculated by taking 75 of the total tooth volume. w w w. c b s s c i e n t i f i c . c o m16 VERTICALS Wide Dual Mini-Verticals Wide Dual Mini-Vertical DCX-800 Complete System Lower buffer reservoir with stirring corral 1 dual-capacity coreupper reservoir Platinum electrodes Safety cover with power leads 2 Blotting cassettes with sponge pads Conversion plate for running one gel at a time DCX-800 series runs or blots two 15cmw x 10cml gels SPECIFICATIONS Number of gels 1-2 Compatible Precast gels NuPAGE Midi-Gels Compatible Precast Gel Sizes cm 15w x 10cml Handcast Glass Plate Dimensions cm 15w x 10cml Required Cathode Buffer Volume 250ml Required Anode Buffer Volume 600ml minimum - 1100ml Slab Gel Run Time 30-90 minutes Blot Run Time 60-90 minutes Recommended Power Supply EPS-300 X or EPX-250 X Overall Dimensions W x D x H cm 22 x 12.7 x 15.4 COMB OPTIONS material PTFE tooth depth 9.5mm overall length 11.8cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MG15-0712M 122 0.75 7.38 3.07 40l 16l marker MG15-0720 20 0.75 4.27 23l MG15-0726 26 0.75 2.80 15l MG15-1012M 122 1.0 7.38 3.07 52l 22l marker MG15-1020 20 1.0 4.27 30l MG15-1026 26 1.0 2.80 20l MG15-1512M 122 1.5 7.38 3.07 78l 33l marker MG15-1520 20 1.5 4.27 45l MG15-1526 26 1.5 2.80 30l PAGE Precast Gels Accessories APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Western Blot Wide Dual Mini-Vertical Cooled DCX-800C Complete System Lower buffer reservoir with cooling base and tubing adapters 1 dual-capacity coreupper reservoir Platinum electrodes Safety cover with power leads 2 Blotting cassettes with sponge pads Conversion plate for running one gel at a time FEATURES Accepts both precast or handcast gels. 50 wider than standard Mini-Gel format allows increased sample volumes and increased number of wells. Dual capacity with dual function - run either 2 slab gels or 2 Western blots. NOTE Electrophoretic transfers do not require the purchase of special transfer modules. Each gel or blotting cassette is placed into a double-gasketed receptacle in the core for increased perimeter pressure ensuring a tight fit and preventing surrounding buffer interference. Hinged core design allows easy loading and removal of gel or blotting cassettes. Wide Dual Mini-Vertical Series cat. s DCX-800 and DCX-800C will accept 15cmw x 10cml precast gels. Alternatively handcasting can be accomplished using Gel Wrap Casting Bundles and Multi-Caster Kits. The DCX-800C provides the capability of running 2 gels or 2 blots simultaneously under identical temperature controlled buffer conditions. This unit has a cooling base and tubing adapters. Optional self-closing quick-disconnect valves cat. QDC-P simplify connection to an external cooler. Gel or blotting cassettes can be extracted from the core without removing the core from the tank allowing for quick processing of samples. This feature also allows for the immediate reuse of buffer for the next set of samples. Color-coded anode and cathode safety features on all components Tank design includes stirring bar corral to maintain even temperature. There are no loose parts to keep track of on the central core. This system can be used for fluorescence based assays whose images can be captured on a scanner directly through optional borosilicate glass plate sets. Maximum loading volume is calculated by taking 75 of the total tooth volume. Combs with M at end are 12 wells with 2 marker lanes p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 17 VERTICALS Precast Gel Options for Wide Dual Mini Verticals PAGE Precast Gels Accessories Precast Gels These superior quality ultra-strong SDS PAGE precast gels have a 2-year shelf life. Available with acrylamide percentages of 12 4-12 and 4-20 with choice of 12 wells 2 marker lanes 20 or 26 wells. Wide format allows higher loading capacity . See pages 58-61 for more information. 12 ClearPAGE SDS precast gel 15cmw x 10cml 12 well 2 marker lanes OPTION 1 Multi-Caster Method 2 Handcast Options for Wide Dual Mini-Verticals Multi-Caster 4-place DCX8-MC4 Complete System Multi-Caster Chamber with lid Silicone gasket 6 stainless steel thumbscrews Backfill port connecting to central filling trough Polycarbonate separation sheets Acrylic shim plate See Product list or website for complete listing of catalog numbers and ordering options Glass plates combs and spacers must be ordered separately. See page 23 for assembly diagram. OPTION 2 Gel Wrap Casting System 2-Place Gel Wrap Casting Bundles See top right of page 11 for series of photos detailing Gel Wrap System. Each casting kit includes Wide Dual Mini-Vertical and all the components to handcast 2 gels. Choice of 0.75mm 1.0mm or 1.5mm thicknesses for combs spacers and Gel Wrap gasket. Wide Dual Mini-Vertical with Gel Wrap Casting Bundle DCX-800-HC DCX-800C-HC Complete System Wide Dual Mini-Vertical System 2 Gel Wrap Gaskets 2 Combs 2 Sets Gel Wrap spacers 2 Sets of Gel Wrap glass plates 8 clamps Lane 1 Chicken lysate Lane 2 Chicken lysate Lane 3 BSA Lane 4 BioRad Markers Lane 5 Chicken lysate Lane 6 Chicken lysate Lane 7 Chicken lysate Lane 8 Chicken lysate Lane 9 E. Coli Lane 10 BioRad Markers Lane 11 BSA Lane 12 Chicken lysate Lane 13 Chicken lysate Lane 14 Chicken lysate ORDERING INFORMATION CAT. DESCRIPTION WIDE DUAL MINI-VERTICALS - 15CMW X 10CMH DCX-800 Wide Dual Mini-Vertical without cooling Complete System DCX-800C Wide Dual Mini-Vertical with cooling base Complete System DCX-800-HC Wide Dual Mini-Vertical without cooling Complete System with 2-place Gel Wrap Casting Bundle DCX-800C-HC Wide Dual Mini-Vertical Dual Midi-Vertical with cooling base Complete System with 2-place Gel Wrap Casting Bundle Accessories and Related Products QDC-P Quick-Disconnect Coupling self-closing EBX-BC-800 Additional Accessory Blotting Cassette includes sponge pad EBX-SP-800 Additional Accessory Sponge Pads set of 4 MGP15-100R Gel Wrap Glass Plate Set MGP15-100R-DN Gel Wrap Glass plate Set with Borosilicate back plate MGE15-0710 Gel Wrap Gasket 0.75mm thick MGE15-1010 Gel Wrap Gasket 1.0mm thick MGE15-1510 Gel Wrap Gasket 1.5mm thick DCX-S0710R Gel Wrap Spacer Set 0.75mm thick DCX-S1010R Gel Wrap Spacer Set 1.0mm thick DCX-S1510R Gel Wrap Spacer Set 1.5mm thick DCX8-MC4 Wide Mini-Vertical Multi-Caster 4-place Unit Only MGP15-100 Multi-Caster Glass Plate Set MGP15-100-DN Multi-Caster Glass plate Set with Borosilicate back plate DCX-S0710 Multi-Caster Spacer Set 0.75mm thick DCX-S1010 Multi-Caster Spacer Set 1.0mm thick DCX-S1510 Multi-Caster Spacer Set 1.5mm thick EBU-402 Double-Wide Blotting System 2 place Includes 1 cassette for up to 20cmw x 9.5cmh gels EBU-4000 Semi-Dry Blotting System transfer area of 20cm x 20cm fits 2 each 15cm x 10cm gels. EPS-300 X Power Supply CE 1-300V 1-500mA EPX-250 X High Current Power Supply CE 2-250V 10-2500mA Blotting Systems Compatible Blotting System for the DCX- 800 Series is the Double Wide Mini-Blotting System EBU-402. This Tank Blotter fits gel dimensions of up to 20cmw x 9.5cmh. This is for the lab that requires a dedicated blotting system. See page 36 for complete description. Compatible Semi-Dry Blotter EBU-4000 is described on page 39. ORDERING INFORMATION ClearPAGE SDS gels- 15cmw x 10cmh packages of 10 - Cat. s acrylamide 12 well 2 marker lanes 20 well 26 well 12 GK-01212-10 GK-01220-10 GK-01226-10 4 - 12 GK-41212-10 GK-41220-10 GK-41226-10 4 - 20 GK-42012-10 GK-42020-10 GK-42026-10 w w w. c b s s c i e n t i f i c . c o m18 VERTICALS APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Double-Wide Mini-Verticals Dual units run the equivalent of 4 standard 10 x 10 mini-gels simultaneously Double-Wide Mini-Vertical Single MGV-102-20 MGV-116-20 Complete System Electrophoresis cell with safety cover and power leads Platinum electrodes Long-lasting silicone gasket White spring clamps 1 comb 1 set of Gel Wrap spacers 1 set of Gel Wrap glass plates 1 length of Gel Wrap gasket COMB OPTIONS for 20cm wide units MGV-102-20 MGV-202-20 MGV-116-20 MGV-216-20 material PTFE tooth depth 9.5mm overall length 15.8cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MVC20-0717 17 0.75 6.7 36 MVC20-0720 20 0.75 5.66 30 MVC20-0728 28 0.75 4 21 MVC20-0732 32 0.75 3.4 18 MVC20-0735 35 0.75 2.95 16 MVC20-0752TT 52 0.75 2 11 MVC20-1017 17 1.0 6.7 48 MVC20-1020 20 1.0 5.66 40 MVC20-1028 28 1.0 4 29 MVC20-1032 32 1.0 3.4 24 MVC20-1035 35 1.0 2.95 21 MVC20-1052TT 52 1.0 2 14 MVC20-1517 17 1.5 6.7 71 MVC20-1520 20 1.5 5.66 60 MVC20-1528 28 1.5 4 43 MVC20-1532 32 1.5 3.4 36 MVC20-1535 35 1.5 2.95 32 MVC20-1552TT 52 1.5 2 21 Double-Wide Mini-Vertical Dual MGV-202-20 MGV-216-20 Complete System Electrophoresis cell with cooling chamber and coolant recirculation ports Platinum electrodes Long-lasting silicone gasket Safety cover with power leads White spring clamps 2 combs 2 sets of Gel Wrap spacers 2 sets of Gel Wrap glass plates 2 lengths of Gel Wrap gasket Conversion plate for running one gel at a time SPECIFICATIONS Model Cat. MGV-102-20 MGV-202-20 MGV-116-20 MGV-216-20 of Gels 1 1-2 1 1-2 Overall Dimensions W x D x H cm 31 x 14 x 13 31 x 18 x 13 31 x 14 x 18 31 x 19 x 18 Glass Plate Dimensions W x L cm 20 x 10 20 x 10 20 x 16 20 x 16 Matching Tank Blotter EBU-402 EBU-402 EBU-102 EBU-102 Matching Semi-Dry Blotter EBU-3000 EBU-3000 EBU-4000 EBU-4000 Recommended Power Supply EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X Recommended Buffer Volume Upper reservoir Lower reservoir 140mls 200mls 240mls 200mls 140mls 200mls 250mls 400mls Slab Gel Run Time 35-40 min. 35-40 min. 30-90 min. 30-90 min. Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. TT tapered tooth FEATURES 20cm wide systems are ideal for electrophoretic separations of a large number of samples that require a relatively short pathlength for resolution. All units are 20cmw. The MGV-102-20 and MGV-202-20 have a 10cm long pathlengths while the MGV-116-20 and MGV-216-20 have 16cm long pathlengths. Internal cooling chamber in dual units provides precise temperature control. Double-wide mini-gels can be cast using our patented Gel Wrap Gasket system or with Multi-Caster Kits. The MGV-202-20 and MGV-216-20 can screen of up to 102 samples simultaneously. The 52 well microtitre-compatible comb is designed to run up to 96 samples plus controls on each side so that results from 1 microtitre plate cans be analyzed simultaneously. This system can be used for fluorescence based assays whose images can be captured on a scanner directly through optional borosilicate glass plate sets. For easy identification the thickness and cat.s are printed on all spacers. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 19 VERTICALS OPTION 1 Multi-Caster Method 2 Handcast Gel Options for Double-Wide Systems OPTION 2 Gel Wrap Casting System Double-Wide Multi-Caster Kit GCC-204-20K 4-place Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See page 23 for assembly diagram 4 sets notched glass plate sets 4 sets of spacers 4 combs Polycarbonate separation sheets Acrylic shim plate C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Each Double-Wide Mini-Vertical comes with 2 gel wrap gaskets and all components necessary to cast 2 gels by this method. See page 23 for series of photos detailing Gel Wrap System. Blotting Systems Double-Wide Mini-Blotting System EBU-402 shown at left is the companion tank blotter for MGV-102-20 and MGV-202-20. See page 36. Large Blotting System EBU-102 shown at right is the companion tank blotter for MGV-116-20 and MGV-216-20. See page 38. ORDERING INFORMATION CAT. DESCRIPTION DOUBLE-WIDE MINI-VERTICALS - 20CMW X 10CMH MGV-102-20 Double-Wide Mini-Vertical Single Complete System gel dimensions 20cmw x 10cmh MGV-202-20 Double-Wide Mini-Vertical Dual Complete System gel dimensions 20cmw x 10cmh Accessories and Replacement parts for MGV-102-20 or MGV-202-20 MGP20-100R Gel Wrap Glass plate Set MGP20-100R-DN Gel Wrap Glass plate Set with Borosilicate back plate MVE20-0710 Gel Wrap Gasket 0.75mm thick MVE20-1010 Gel Wrap Gasket 1.0mm thick MVE20-1510 Gel Wrap Gasket 1.5mm thick MVS-0710R Gel Wrap Spacer Set 0.75mm thick MVS-1010R Gel Wrap Spacer Set 1.0mm thick MVS-1510R Gel Wrap Spacer Set 1.5mm thick GCC-204-20K Double-Wide Multi-Caster Kit 4-place MGP20-100 Multi-Caster Glass plate Set MGP20-100-DN Multi-Caster Glass plate Set with Borosilicate back plate MVS-0710 Multi-Caster Spacer Set 0.75mm thick MVS-1010 Multi-Caster Spacer Set 1.0mm thick MVS-1510 Multi-Caster Spacer Set 1.5mm thick EBU-402 Double-Wide Blotting System 2-place Includes 1 cassette 20cm x 9.5cm EBC-402 Additional Blotting Cassette for EBU-402 EBU-3000 Semi-Dry Blotting System transfer area of 12cm x 34cm EPS-300 X Power Supply CE 1-300V 1-500mA CAT. DESCRIPTION DOUBLE-WIDE MINI-VERTICALS - 20CMW X 16CMH MGV-116-20 Double-Wide Mini-Vertical Single Complete System gel dimensions 20cmw x 16cmh MGV-216-20 Double-Wide Mini-Vertical Dual Complete System gel dimensions 20cmw x 16cmh Accessories and Replacement parts for MGV-116-20 or MGV-216-20 MGP20-160R Gel Wrap Glass plate Set MGP20-160R-DN Gel Wrap Glass plate Set with Borosilicate back plate MVE20-0716 Gel Wrap Gasket 0.75mm thick MVE20-1016 Gel Wrap Gasket 1.0mm thick MVE20-1516 Gel Wrap Gasket 1.5mm thick MVS-0716R Gel Wrap Spacer Set 0.75mm thick MVS-1016R Gel Wrap Spacer Set 1.0mm thick MVS-1516R Gel Wrap Spacer Set 1.5mm thick GCC-216-20K Double-Wide Multi-Caster Kit 4-place MGP20-160 Multi-Caster Glass Plate Set MGP20-160-DN Multi-Caster Glass plate Set with Borosilicate back plate MVS-0716 Multi-Caster Spacer Set 0.75mm thick MVS-1016 Multi-Caster Spacer Set 1.0mm thick MVS-1516 Multi-Caster Spacer Set 1.5mm thick EBU-102 Large Blotting System 3-place Includes 1 cassette 22cm x 16cm EBC-102 Additional Blotting Cassette for EBU-102 EBU-4000 Semi-Dry Blotting System transfer area of 20cm x 20cm EPS-300 X Power Supply CE 1-300V 1-500mA Semi-Blotting Systems EBU-3000 shown at right and EBU-4000 are the companion Semi-Dry Blotters for the Double- Wide Vertical Mini-Gel Systems. See page 39 for complete description. w w w. c b s s c i e n t i f i c . c o m20 VERTICALS APPLICATIONS SDS-PAGE Native PAGE Nucleic Acid PAGE Screening of large numbers of samples Triple-Wide Mini-Verticals Single MGV-102-33 MGV-116-33 Complete System Electrophoresis cell with safety cover and power leads Platinum electrodes Long-lasting silicone gasket White spring clamps 1 comb 1 set of Gel Wrap spacers 1 set of Gel Wrap glass plates 1 length of Gel Wrap gasket Triple-Wide Mini-Verticals High-Throughput Mini-Gel screens up to 204 samples simultaneously SPECIFICATIONS Model Cat. MGV-102-33 MGV-202-33 MGV-116-33 MGV-216-33 Model Type Single Dual Single Dual Overall Dimensions W x D x H cm 45 x 14 x 12 45 x 19 x 12 45 x 14 x 18 45 x 19 x 18 Glass Plate Dimensions W x L cm 33 x 10 33 x 10 33 x 16 33 x 16 Matching Tank Blotter Cat. EBU-302 EBU-302 EBU-362 EBU-362 Matching Semi-Dry Blotter Cat. EBU-3000 EBU-3000 EBU-6000 EBU-6000 Recommended Power Supply EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X EPS-300 X or EPX-250 X Recommended Buffer Volume Upper reservoir Lower reservoir 240mls 200mls 250mls 400mls 240mls 200mls 250mls 400mls Slab Gel Run Time 30-90 min. 30-90 min. 60-90 min. 60-90 min. FEATURES Dual or Single Units are available in 2 gel sizes 33cm w x 10cm h with matching Blotter EBU-302 or 33cmw x 16cmh with matching Blotter EBU-362. 33cm wide systems are ideal for electrophoretic separations of a large number of samples that require a relatively short pathlength for resolu- tion. Internal cooling chamber in dual units provides precise temperature control. For easy identification the thickness and catalog are printed on all combs and spacers. The MGV-202-33 MGV-216-33 are specifically designed for the mass screening of up to 204 samples simultaneously. These systems can be used for fluorescence based assays whose images can be captured on a scanner directly through optional borosilicate glass plate sets. The 102 well microtitre-compatible comb is designed to run 96 samples plus controls on each side so that results from 2 microtitre plates cans be analyzed simultaneously. Triple-Wide Mini-Verticals Dual MGV-202-33 MGV-216-33 Complete System Electrophoresis cell with cooling chamber and coolant recirculation ports Platinum electrodes Long-lasting silicone gaskets Safety cover with power leads White spring clamps 2 combs 2 sets of Gel Wrap spacers 2 sets of Gel Wrap glass plates 2 lengths of Gel Wrap gasket Conversion plate for running one gel at a time COMB OPTIONS for 33cm wide units MGV-202-33 MGV-216-33 MGV-116-33 MGV-216-33 material PTFE tooth depth 10mm overall length 28.4cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MG33-0731 31 0.75 5.8 33 MG33-0750 50 0.75 4.05 23 MG33-0763 63 0.75 2.95 17 MG33-07102TT 102 0.75 2 11 MG33-1031 31 1.0 5.8 44 MG33-1050 50 1.0 4.05 30 MG33-1063 63 1.0 2.95 23 MG33-10102TT 102 1.0 2 15 MG33-1531 31 1.5 5.8 65 MG33-1550 50 1.5 4.05 45 MG33-1563 63 1.5 2.95 33 MG33-15102TT 102 1.5 2 23 Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. TT tapered tooth p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 21 VERTICALS OPTION 1 Multi-Caster Method 2 Handcast Gel Options for Triple-Wide Systems OPTION 2 Gel Wrap Casting System Triple-Wide Multi-Caster Kits 4-place GCC-204-33K GCC-264-33K Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See page 23 for assembly diagram 4 sets notched glass plate sets 4 sets of spacers 4 combs Polycarbonate separation sheets Acrylic shim plate C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Each Triple-Wide Mini-Vertical comes with 2 gel wrap gaskets and all components necessary to cast 2 gels by this method. See page 23 for series of photos detailing Gel Wrap Casting System. Blotting Systems Triple-Wide Mini-Blotting System EBU-302 is the companion Tank Blotter for Triple-Wide Systems MGV- 102-33 and MGV-202-33. Triple- Wide Mini-Blotting System EBU-362 is the matching blotter for MGV-116- 33 and MGV-216-33. See page 37 for complete description. ORDERING INFORMATION CAT. DESCRIPTION TRIPLE-WIDE MINI-VERTICALS - 33CMW X 10CMH MGV-102-33 Triple-Wide Mini-Vertical Single Complete System gel dimensions 33cmw x 10cmh MGV-202-33 Triple-Wide Mini-Vertical Dual Complete System gel dimensions 33cmw x 10cmh Accessories and Replacement parts for MGV-102-33 or MGV-202-33 MGP33-100R Gel Wrap Glass plate Set MGP33-100R-DN Gel Wrap Glass plate Set with Borosilicate back plate MGE33-0710 Gel Wrap Gasket 0.75mm thick MGE33-1010 Gel Wrap Gasket 1.0mm thick MGE33-1510 Gel Wrap Gasket 1.5mm thick MVS-0710R Gel Wrap Spacer Set 0.75mm thick MVS-1010R Gel Wrap Spacer Set 1.0mm thick MVS-1510R Gel Wrap Spacer Set 1.5mm thick GCC-204-33K Triple-Wide Multi-Caster Kit 4-place for 10cmh gels MGP33-100 Multi-Caster Glass plate Set MGP33-100-DN Multi-Caster Glass plate Set with Borosilicate back plate MVS-0710 Multi-Caster Spacer Set 0.75mm thick MVS-1010 Multi-Caster Spacer Set 1.0mm thick MVS-1510 Multi-Caster Spacer Set 1.5mm thick EBU-302 Triple-Wide Blotting System 2-place Includes 1 cas- sette fits 33cmw x 9.5cmh gels. EBC-302 Additional Blotting Cassette for EBU-302 EBU-3000 Semi-Dry Blotting System transfer area of 12cm x 34cm EPS-300 X Power Supply CE 1-300V 1-500mA EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA CAT. DESCRIPTION TRIPLE-WIDE MINI-VERTICALS - 33CMW X 16CMH MGV-116-33 Triple-Wide Mini-Vertical Single Complete System gel dimensions 33cmw x 16cmh MGV-216-33 Triple-Wide Mini-Vertical Dual Complete System gel dimensions 33cmw x 16cmh Accessories and Replacement parts for MGV-116-33 or MGV-216-33 MGP33-160R Gel Wrap Glass plate Set MGP33-160R-DN Gel Wrap Glass plate Set with Borosilicate back plate MGE33-0716 Gel Wrap Gasket 0.75mm thick MGE33-1016 Gel Wrap Gasket 1.0mm thick MGE33-1516 Gel Wrap Gasket 1.5mm thick MVS-0716R Gel Wrap Spacer Set 0.75mm thick MVS-1016R Gel Wrap Spacer Set 1.0mm thick MVS-1516R Gel Wrap Spacer Set 1.5mm thick GCC-264-33K Triple-Wide Multi-Caster Kit 4-place for 16cmh gels MGP33-160 Multi-Caster Glass Plate Set MGP33-160-DN Multi-Caster Glass plate Set with Borosilicate back plate MVS-0716 Multi-Caster Spacer Set 0.75mm thick MVS-1016 Multi-Caster Spacer Set 1.0mm thick MVS-1516 Multi-Caster Spacer Set 1.5mm thick EBU-362 Triple-Wide Blotting System 2-place Includes 1 cas- sette fits 33cmw x 13cmh gels. EBC-362 Additional Blotting Cassette for EBU-362 EBU-6000 Semi-Dry Blotting System transfer area of 35cm x 40cm EPS-300 X Power Supply CE 1-300V 1-500mA EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA Compatible Semi- Blotting Systems EBU- 3000 shown at left and EBU-6000 are described on page 39. w w w. c b s s c i e n t i f i c . c o m22 VERTICALS Economical and easy to use with a choice of 6 heights C.B.S. Lite Vertical Systems SG-125 SG-125-02 SG-160 SG-160-02 SG-170 SG-170-02 SG-190 SG-190-02 SG-200 SG-200-02 SG-250 SG-250-02 Complete System Vertical electrophoresis cell with leveling base and bubble level Safety covers and power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes 1 comb 1 sets Gel Wrap glass plates 1 set of Gel Wrap spacers 1 Gel Wrap gasket for gel casting White spring clamps FEATURES Single Slab Gel Systems are available in 6 heights. C.B.S. Lite System has a cut-out upright for air cooling A CEEU version of safety cover is available for all C.B.S. Lite Systems. To specify the CE version add -02 to the end of the catalog number. Glass plates to fit the six sizes offered are 16.5cmw with heights of 145mm 160mm 170mm 190mm 220mm 280mm and can be ordered with a low fluorescent borosilicate back plate. Ideal for fluores- cence based assays Spacer sets are offered in thicknesses of 0.75mm 1.0mm 1.5mm 2.0mm and 3.0mm. For full listing of accessories please consult our website or product list. APPLICATIONS SDS PAGE Native PAGE Nucleic Acid PAGE C.B.S. Lite Vertical Systems Blotting Systems The EBU-102 Blotting System provides the capability of transferring 3 gels simultaneously under identical temperature controlled buffer conditions. This vertical blotting system can accommodate gels of heights up to 22cm. See page 38 for complete description. Compatible Semi-Dry Blotter EBU-4000 is described on page 39. Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. SPECIFICATIONS Cat. Overall Dimensions W x D x H cm Glass Plate Dimensions W x L cm Recommended Reservoir Buffer Volume mls SG-125-02 22 x 19 x 22 16.5 x 14.5 300 upper 125-200 lower SG-160-02 22 x 19 x 23 16.5 x 16 300 upper 125-200 lower SG-170-02 22 x 19 x 24 16.5 x 17 300 upper 125-200 lower SG-190-02 22 x 19 x 26 16.5 x 19 300 upper 125-200 lower SG-200-02 22 x 19 x 29 16.5 x 22 300 upper 125-200 lower SG-250-02 22 x 19 x 48 16.5 x 28 300 upper 125-200 lower COMB OPTIONS for 16.5 cm wide units material PTFE overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Tooth Width mm Recommended Max. Sample Vol. Well l VGC-0710 19.1 10 0.75 10.1 109 VGC-0712 19.1 12 0.75 7.9 85 VGC-0714 19.1 14 0.75 6.4 68 VGC-0715 19.1 15 0.75 5.7 62 VGC-0716 19.1 16 0.75 5.2 56 VGC-0720 17.7 20 0.75 4.2 42 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1010 19.1 10 1.0 10.1 146 VGC-1012 19.1 12 1.0 7.9 113 VGC-1014 19.1 14 1.0 6.4 90 VGC-1015 19.1 15 1.0 5.7 82 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 56 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1510 17.7 10 1.5 10.1 218 VGC-1512 19.1 12 1.5 7.9 170 VGC-1514 19.1 14 1.5 6.4 137 VGC-1515 19.1 15 1.5 5.7 122 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 VGC-2010 19.1 10 2.0 10.1 292 VGC-2012 19.1 12 2.0 7.9 226 VGC-2014 19.1 14 2.0 6.4 180 VGC-2015 19.1 15 2.0 5.7 164 VGC-2016 19.1 16 2.0 5.2 148 VGC-2020 17.7 20 2.0 4.2 112 VGC-2029 17.7 29 2.0 2.9 76 VGC-2030 17.7 30 2.0 2.8 74 VGC-3010 19.1 10 3.0 10.1 438 VGC-3012 19.1 12 3.0 7.9 339 VGC-3014 19.1 14 3.0 6.4 270 VGC-3015 19.1 15 3.0 5.7 246 VGC-3016 19.1 16 3.0 5.2 222 VGC-3020 17.7 20 3.0 4.2 168 VGC-3029 17.7 29 3.0 2.9 114 VGC-3030 17.7 30 3.0 2.8 111 p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 23 VERTICALS OPTION 2 Gel Wrap Casting System C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. Gel Casting Options OPTION 1 Multi-Caster Method 5 Seconds 15 Seconds 25 Seconds 60 Seconds Disassembly 45 Seconds separation sheet notched glass plate spacer unnotched glass plate shim sheet front panel with captive thumbscrews casting chamber lid used to tilt assembly separation sheet Multi-Caster Assembly Diagram Diagram shown below depicts proper assembly for Multiple Gel Casting Chamber GCC-404-K. See Product List or website for complete listing of catalog numbers and ordering options ORDERING INFORMATION CAT. DESCRIPTION C.B.S. LITE VERTICAL SYSTEMS - 16.5CMW SG-125 C.B.S. Lite Vertical System Complete System glass plate height 14.5cm SG-125-02 C.B.S. Lite Vertical System CE Complete System glass plate height 14.5cm SG-160 C.B.S. Lite Vertical System Complete System glass plate height 16cm SG-160-02 C.B.S. Lite Vertical System CE Complete System glass plate height 16cm SG-170 C.B.S. Lite Vertical System Complete System glass plate height 17cm SG-170-02 C.B.S. Lite Vertical System CE Complete System glass plate height 17cm SG-190 C.B.S. Lite Vertical System Complete System glass plate height 19cm SG-190-02 C.B.S. Lite Vertical System CE Complete System glass plate height 19cm SG-200 C.B.S. Lite Vertical System Complete System glass plate height 22cm SG-200-02 C.B.S. Lite Vertical System CE Complete System glass plate height 22cm SG-250 C.B.S. Lite Vertical System Complete System glass plate height 28cm SG-250-02 C.B.S. Lite Vertical System CE Complete System glass plate height 28cm Accessories and Related Products for Slab Gel Kits GCC-404K Multi-Caster Kit 4-place 16.5cmw fits heights up to 22cm GCC-410K Multi-Caster Kit 10-place 16.5cmw fits heights up to 22cm EBU-102 Blotting System 3-place fits gel heights up to 22cm. Includes 1 gel cassette EBC-102 Additional Blotting Cassette for EBU-102 EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 Power Supply CE 5-600V 1-500mA Online video instructions for both Gel Wrap and Multi-Caster methods can be found at httpwww.cbsscientific.cominstructionvideos.aspx. Multi-Caster Kits 16.5cmw GCC-404K 4-place GCC-410K 10-place Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See assembly diagram below 4 each 4-place or 10 each 10-place notched glass plate sets spacers and combs Polycarbonate separation sheets Acrylic shim plate w w w. c b s s c i e n t i f i c . c o m24 VERTICALS Cooled Dual Vertical with a choice of 6 heights SPECIFICATIONS Cat. Overall Dimensions cm W x D x H cm Glass Plate Dimensions W x L cm Recommended Buffer Reservoir Volume mls DSG-125-02 24 x 23 x 22 16.5 x 14.5 300 upper 125-200 lower DSG-160-02 24 x 23 x 23 16.5 x 16 300 upper 125-200 lower DSG-170-02 24 x 23 x 24 16.5 x 17 300 upper 125-200 lower DSG-190-02 24 x 23 x 26 16.5 x 19 300 upper 125-200 lower DSG-200-02 24 x 23 x 29 16.5 x 22 300 upper 125-200 lower DSG-250-02 24 x 23 x 35 16.5 x 28 300 upper 125-200 lower DSG Dual Vertical Systems DSG-125-02 DSG-160-02 DSG-170-02 DSG-190-02 DSG-200-02 DSG-250-02 Complete System Dual Vertical electrophoresis cell with internal cooling chamber leveling base and bubble level Safety cover and power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes 2 combs 2 sets Gel Wrap glass plates 2 sets of Gel Wrap spacers 2 Gel Wrap gaskets for gel casting White spring clamps Conversion plate for running one gel at a time FEATURES Dual Slab Gel Systems are available in 6 heights. Two gels can be run simultaneously under identical buffer conditions Cooling chamber assures constant temperature during electrophoresis A CEEU safety cover is standard with all DSG Dual Vertical Slab Gel Systems. Glass plates can be ordered with a low fluorescent borosilicate back plate. Ideal for fluorescence based assays. Spacer sets are offered in thicknesses of 0.75mm 1.0mm 1.5mm 2.0mm and 3.0mm. For full listing of accessories please consult our website or product list. DSG Dual Vertical Systems Blotting Systems The EBU-102 Blotting System provides the capability of transferring 3 gels simultaneously under identical temperature controlled buffer conditions. Temperature control is achieved by passing coolant through the base of the blotter. This vertical blotting system can accommodate gels of dimensions up to 16cmw x 22cmh. See page 38 for complete description. APPLICATIONS SDS PAGE Native PAGE Nucleic Acid PAGE Semi-Dry Blotting System EBU-4000 fits all DSG gels with glass plate heights up to 22cm. See page 39 for complete description. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 25 VERTICALS OPTION 2 Gel Wrap Casting System C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. See page 23 for series of photos detailing Gel Wrap System. Gel Casting Options OPTION 1 Multi-Caster Method See Product List or website for complete listing of catalog numbers and ordering options ORDERING INFORMATION CAT. DESCRIPTION DSG DUAL VERTICAL SYSTEMS - 16.5CMW DSG-125-02 Dual Vertical System CE Complete System glass plate height 14.5cm DSG-160-02 Dual Vertical System CE Complete System glass plate height 16cm DSG-170-02 Dual Vertical System CE Complete System glass plate height 17cm DSG-190-02 Dual Vertical System CE Complete System glass plate height 19cm DSG-200-02 Dual Vertical System CE Complete System glass plate height 22cm DSG-250-02 Dual Vertical System CE Complete System glass plate height 28cm Accessories and Related Products for Dual Vertical Systems GCC-404K Multi-Caster Kit 4-place 16.5cmw fits heights up to 22cm GCC-410K Multi-Caster Kit 10-place 16.5cmw fits heights up to 22cm EBU-102 Blotting System 3-place fits gel heights up to 22cm. Includes 1 gel cassette. EBC-102 Additional Blotting Cassette for EBU-102 EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 Power Supply CE 5-600V 1-500mA Online video instructions can be found at httpwww.cbsscientific.com instructionvideos.aspx. Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. COMB OPTIONS for 16.5 cm wide units material PTFE overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Tooth Width mm Recommended Max. Sample Vol. Well l VGC-0710 19.1 10 0.75 10.1 109 VGC-0712 19.1 12 0.75 7.9 85 VGC-0714 19.1 14 0.75 6.4 68 VGC-0715 19.1 15 0.75 5.7 62 VGC-0716 19.1 16 0.75 5.2 56 VGC-0720 17.7 20 0.75 4.2 42 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1010 19.1 10 1.0 10.1 146 VGC-1012 19.1 12 1.0 7.9 113 VGC-1014 19.1 14 1.0 6.4 90 VGC-1015 19.1 15 1.0 5.7 82 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 56 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1510 17.7 10 1.5 10.1 218 VGC-1512 19.1 12 1.5 7.9 170 VGC-1514 19.1 14 1.5 6.4 137 VGC-1515 19.1 15 1.5 5.7 122 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 VGC-2010 19.1 10 2.0 10.1 292 VGC-2012 19.1 12 2.0 7.9 226 VGC-2014 19.1 14 2.0 6.4 180 VGC-2015 19.1 15 2.0 5.7 164 VGC-2016 19.1 16 2.0 5.2 148 VGC-2020 17.7 20 2.0 4.2 112 VGC-2029 17.7 29 2.0 2.9 76 VGC-2030 17.7 30 2.0 2.8 74 VGC-3010 19.1 10 3.0 10.1 438 VGC-3012 19.1 12 3.0 7.9 339 VGC-3014 19.1 14 3.0 6.4 270 VGC-3015 19.1 15 3.0 5.7 246 VGC-3016 19.1 16 3.0 5.2 222 VGC-3020 17.7 20 3.0 4.2 168 VGC-3029 17.7 29 3.0 2.9 114 VGC-3030 17.7 30 3.0 2.8 111 Multi-Caster Kits 16.5cmw GCC-404K 4-place GCC-410K 10-place Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See assembly diagram on page 23 4 each 4-place or 10 each 10-place notched glass plate sets spacers and combs Polycarbonate separation sheets Acrylic shim plate w w w. c b s s c i e n t i f i c . c o m26 VERTICALS Adjustable Vertical Systems Adjustable Height Dual or Single Systems adapt to changing methodologies Dual Adjustable Vertical Systems DASG-250 DASG-250-02 DASG-400 DASG-400-02 Complete System Dual vertical electrophoresis cell with delrin rods leveling base and bubble level Safety covers and power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes 2 combs 2 sets Gel Wrap glass plates 2 sets of Gel Wrap spacers 2 Gel Wrap gaskets for gel casting 2 wedge plate separators White spring clamps Conversion plate for running one gel at a time Adjustable Vertical Systems ASG-250 ASG-250-02 ASG-400 ASG-400-02 Complete System Single vertical electrophoresis cell with delrin rods safety covers power leads and leveling base with bubble level Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes 1 comb 1 set Gel Wrap glass plates 1 set of Gel Wrap spacers 1 Gel Wrap gasket for gel casting 1 wedge plate separator White spring clamps FEATURES Single and Dual Adjustable Slab Gel Systems are designed to meet a variety of applications. The adjustable upper buffer reservoir rides on two delrin rods and is secured in place by two stainless steel hand screws providing the flexibility of running 16.5cm wide gels in lengths from 145mm up to 280mm ASG-250 or DASG-250 or from 145mm up to 387mm ASG- 400 or DASG-400. The Dual Adjustable Slab Gel System allows two gels to be run simulta- neously under identical buffer conditions. A CEEU version of safety cover is available for both the single and dual adjustable units not shown. To specify the CE version order the Adjustable Units with -02 at the end of the catalog number. 16.5cm wide glass plates are available in seven lengths 145mm 160mm 170mm 190mm 220mm 280mm and 387mm and can be ordered with front plate beveled for two-dimensional electrophoresis. Spacer sets are offered in thicknesses of 0.75mm 1.0mm 1.5mm 2.0mm and 3.0mm. For full listing of accessories please consult our website or product list. SPECIFICATIONS Vertical System Cat. of Gels Overall Dimensions W x D x H cm Glass Plate Dimension Options W x L cm Recommended Buffer Reservoir Volume mls ASG-250 ASG-250-02 1 22 x 19 x 28 16.5 x 14.5 16.5 x 16 16.5 x 17 16.5 x 19 16.5 x 22 16.5 x 28 310-375 upper 150-350 lower DASG-250 DASG-250-02 2 22 x 19 x 28 16.5 x 14.5 16.5 x 16 16.5 x 17 16.5 x 19 16.5 x 22 16.5 x 28 375-475 upper 150-350 lower ASG-400 ASG-400-02 1 22 x 19 x 42 16.5 x 14.5 16.5 x 16 16.5 x 17 16.5 x 19 16.5 x 22 16.5 x 28 16.5 x 38.7 310-375 upper 150-350 lower DASG-400 DASG-400-02 2 22 x 19 x 42 16.5 x 14.5 16.5 x 16 16.5 x 17 16.5 x 19 16.5 x 22 16.5 x 28 16.5 x 38.7 375-475 upper 150-350 lower APPLICATIONS SDS PAGE Native PAGE Nucleic Acid PAGE p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 27 VERTICALS See Product List or website for complete listing of catalog numbers and ordering options ORDERING INFORMATION CAT. DESCRIPTION ADJUSTABLE VERTICAL SYSTEMS - 16.5CMW ASG-250 Adjustable Vertical System Complete System adjusts from 14.5cm-28cm ASG-250-02 Adjustable Vertical System CE Complete System adjusts from 14.5cm-28cm ASG-400 Adjustable Vertical System Complete System adjusts from 14.5cm-40cm ASG-400-02 Adjustable Vertical System CE Complete System adjusts from 14.5cm-40cm DASG-250 Dual Adjustable Vertical System Complete System adjusts from 14.5cm-28cm DASG-250-02 Dual Adjustable Vertical System CE Complete System adjusts from 14.5cm-28cm DASG-400 Dual Adjustable Vertical System Complete System adjusts from 14.5cm-40cm DASG-400-02 Dual Adjustable Vertical System CE Complete System adjusts from 14.5cm-40cm Accessories and Related Products for Adjustable Slab Gel Kits GCC-404K Multi-Caster Kit 4-place 16.5cmw fits heights up to 22cm GCC-410K Multi-Caster Kit 10-place 16.5cmw fits heights up to 22cm EBU-102 Blotting System 3-place fits gel heights up to 22cm. Includes 1 gel cassette EBC-102 Additional Blotting Cassette for EBU-102 EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 Power Supply CE 5-600V 1-500mA Blotting Systems The EBU-102 Blotting System provides the capability of transferring 3 gels simultaneously under identical temperature controlled buffer conditions. Compatible Semi-Dry Blotter EBU-4000 is described on page 39. Both of these Blotting Systems can accommodate gels with glass plate dimensions up to 16cmw x 22cmh. See pages 38-39 for complete descriptions. OPTION 2 Gel Wrap Casting System C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. See page 23 for series of photos detailing Gel Wrap System. Gel Casting Options OPTION 1 Multi-Caster Method COMB OPTIONS for 16.5 cm wide units material PTFE overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Tooth Width mm Recommended Max. Sample Vol. Well l VGC-0710 19.1 10 0.75 10.1 109 VGC-0712 19.1 12 0.75 7.9 85 VGC-0714 19.1 14 0.75 6.4 68 VGC-0715 19.1 15 0.75 5.7 62 VGC-0716 19.1 16 0.75 5.2 56 VGC-0720 17.7 20 0.75 4.2 42 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1010 19.1 10 1.0 10.1 146 VGC-1012 19.1 12 1.0 7.9 113 VGC-1014 19.1 14 1.0 6.4 90 VGC-1015 19.1 15 1.0 5.7 82 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 56 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1510 17.7 10 1.5 10.1 218 VGC-1512 19.1 12 1.5 7.9 170 VGC-1514 19.1 14 1.5 6.4 137 VGC-1515 19.1 15 1.5 5.7 122 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 VGC-2010 19.1 10 2.0 10.1 292 VGC-2012 19.1 12 2.0 7.9 226 VGC-2014 19.1 14 2.0 6.4 180 VGC-2015 19.1 15 2.0 5.7 164 VGC-2016 19.1 16 2.0 5.2 148 VGC-2020 17.7 20 2.0 4.2 112 VGC-2029 17.7 29 2.0 2.9 76 VGC-2030 17.7 30 2.0 2.8 74 VGC-3010 19.1 10 3.0 10.1 438 VGC-3012 19.1 12 3.0 7.9 339 VGC-3014 19.1 14 3.0 6.4 270 VGC-3015 19.1 15 3.0 5.7 246 VGC-3016 19.1 16 3.0 5.2 222 VGC-3020 17.7 20 3.0 4.2 168 VGC-3029 17.7 29 3.0 2.9 114 VGC-3030 17.7 30 3.0 2.8 111 Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. Multi-Caster Kits 16.5cmw GCC-404K 4-place GCC-410K 10-place Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See assembly diagram on page 23 4 each 4-place or 10 each 10-place notched glass plate sets spacers and combs Polycarbonate separation sheets Acrylic shim plate w w w. c b s s c i e n t i f i c . c o m28 VERTICALS Large Format Cooled Gel System Temperature controlled large capacity System for SDS PAGE and 2D analysis SPECIFICATIONS Overall Dimensions cm W x D x H cm 24.2 x 12.4 x 27 Glass Plate Dimensions W x L cm 20 x 22 Recommended Power Supply EPS-600 Recommended Blotting Systems EBU-222 Tank Blotting System EBU-4000 Semi-Dry Blotting System Precast Gels Jule Incorporated Anode Buffer Volume for 2 Gels 800mls minimum - 3.2 liters Cathode Buffer Volume 1 liter Cooled Large Format Gel System CE DCX-900C Complete System Lower buffer reservoir with double-sided cooling labyrinth tubing adapters and stirring corral 1 dual-capacity coreupper reservoir Platinum Electrodes Safety cover with power leads 2 sets of Gel Wrap Gaskets 2 sets of Gel Wrap Spacers 2 sets of Gel Wrap Glass Plates 2 combs Conversion plate for running one gel at a time White spring clamps APPLICATIONS SDS PAGE Native PAGE Nucleic Acid PAGE 2D PAGE IPG Strips FEATURES Increased gel size of 20cmw x 22cml allows larger sample volumes increased number of wells and longer migration distance. The DCX-900C is designed to accept 7cm 11cm 17cm or 18cm IPG strips for 2 dimensional protein analysis. Gel sandwiches are placed into a double-gasketed receptacle in the core for increased perimeter pressure ensuring a tight fit and preventing surrounding buffer interference. Hinged core design allows easy loading and removal of gel sandwiches. Gel casting can be accomplished using Gel Wrap Casting kits or a Multiple Gel Casting Chamber. Gel sandwiches can be extracted from the core without removing the core from the tank allowing for quick processing of samples. This feature also allows for the immediate reuse of buffer for the next set of samples. Double-sided cooling labyrinth provides the capability of running 2 gels simultaneously under identical temperature controlled buffer conditions. Color-coded anode and cathode safety features on all components Tank design includes stirring bar corral There are no loose parts to keep track of on the central core This system can be used for fluorescence based assays whose images can be captured on a scanner directly through optional borosilicate glass plates. Blotting Systems The EBU-222 Large Tank Blotting System provides the capability of transferring 2 gels simultaneously under identical temperature controlled buffer conditions. Temperature control is achieved by passing coolant through the base of the blotter. This vertical blotting system can accommodate gels of dimensions up to 20cmw x 20cmh. See page 38 for complete description. Semi-Blotting System EBU-4000 shown at left has a transfer area of 20cm x 20cm. See page 39 for complete description. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 29 VERTICALS ORDERING INFORMATION CAT. DESCRIPTION COOLED LARGE FORMAT GEL SYSTEM- 20CMW DCX-900C Cooled Large Format Gel System CE Complete System glass plate dimensions 20cmw x 22cml Accessories and Related Products for Cooled Large Format Gel System SGP20-020R Gel Wrap Glass Plate Set SGP20-020R-DN Gel Wrap Glass plate Set with Borosilicate back plate VGE20-0720 Gel Wrap Gasket 0.75mm thick VGE20-1020 Gel Wrap Gasket 1.0mm thick VGE20-1520 Gel Wrap Gasket 1.5mm thick SGS-20-0720R Gel Wrap Spacer Set 0.75mm thick SGS-20-1020R Gel Wrap Spacer Set 1.0mm thick SGS-20-1520R Gel Wrap Spacer Set 1.5mm thick DCX9-MC-4 DCX-900 Multi-Caster 4-place Unit Only fits 4 sets of glass plates 20cmw x 22cml SGP20-020 Multi-Caster Glass Plate Set SGP20-020-DN Multi-Caster Glass plate Set with Borosilicate back plate SGS-20-0720 Multi-Caster Spacer Set 0.75mm thick SGS-20-1020 Multi-Caster Spacer Set 1.0mm thick SGS-20-1520 Multi-Caster Spacer Set 1.5mm thick EBU-222 Large Blotting System 2-place fits gel up to 20cmw x 20cml. Includes 1 gel cassette. EBC-222 Additional Blotting Cassette for EBU-222 EBU-4000 Semi-Dry Blotting System transfer area of 20cm x 20cm EPS-600 Power Supply CE 5-600V 1-500mA EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA OPTION 2 The Gel Wrap Casting System C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. See page 23 for series of photos detailing Gel Wrap System. Gel Casting Options OPTION 1 Multi-Caster Method See Product List or website for complete listing of catalog numbers and ordering options Online video instructions for both Gel Wrap and Multi-Caster methods can be found at httpwww.cbsscientific.cominstructionvideos.aspx. Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. Comb configuration is one large lane and one marker lane Glass plates combs and spacers must be ordered separately. Multi-Caster 20cmw DCX9-MC-4 4-place Complete System Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough See assembly diagram on page 23 Polycarbonate separation sheets Acrylic shim plate COMB OPTIONS THICKER material PTFE overall length 15.87cm Cat. of Teeth Tooth Depth mm Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG20-0701-2D 1 17.45 0.75 158 844 SG20-0702-2D 2 17.45 0.75 1474.2 78522 SG20-0708 8 19.05 0.75 17.7 95 SG20-0712 12 19.05 0.75 11 58 SG20-0717 17 19.05 0.75 6.63 35 SG20-0720 20 19.05 0.75 5.66 30 SG20-0728 28 17.45 0.75 4 22 SG20-0732 32 17.45 0.75 3.4 18 SG20-0735 35 17.45 0.75 2.9 14 SG20-1001-2D 1 17.45 1.0 1125 1125 SG20-1002-2D 2 17.45 1.0 1474.2 104730 SG20-1008 8 19.05 1.0 17.7 126 SG20-1012 12 19.05 1.0 11 78 SG20-1017 17 19.05 1.0 6.63 50 SG20-1020 20 19.05 1.0 5.66 40 SG20-1028 28 17.45 1.0 4 29 SG20-1032 32 17.45 1.0 3.4 24 SG20-1035 35 17.45 1.0 2.9 19 SG20-1501-2D 1 17.45 1.5 158 1688 SG20-1502-2D 2 17.45 1.5 1474.2 157045 SG20-1508 8 19.05 1.5 17.7 190 SG20-1512 12 19.05 1.5 11 117 SG20-1517 17 19.05 1.5 6.63 71 SG20-1520 20 19.05 1.5 5.66 60 SG20-1528 28 17.45 1.5 4 43 SG20-1532 32 17.45 1.5 3.4 36 SG20-1535 35 17.45 1.5 2.9 29 w w w. c b s s c i e n t i f i c . c o m30 VERTICALS Large Format Adjustable Verticals Run large format protein or nucleic acid gels on these versatile units Large Format Dual Adjustable Vertical System for Protein Applications LDASG-400-20 Complete System Dual vertical electrophoresis cell with heavy duty guide rods removable upper and lower reservoirs safety covers with power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes Leveling base with bubble level 2 combs 2 sets Gel Wrap glass plates 2 sets Gel Wrap spacers 2 Gel Wrap gaskets for gel casting 2 wedge plate separators White spring clamps Conversion plate for running one gel at a time Large Format Adjustable Vertical System for Protein Applications LSG-400-20 Complete System Single vertical electrophoresis cell with heavy duty guide rods removable upper and lower reservoirs safety covers with power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes Leveling base with bubble level 1 comb 1 set Gel Wrap glass plates 1 set of Gel Wrap spacers 1 Gel Wrap Gasket for gel casting 1 wedge plate separator White spring clamps FEATURES Unsurpassed versatility - designed to adapt to changing methodologies. Can run 3 different length gels preparative gels large format SDS-PAGE and DNARNA gels or sequencing gels on the same unit by ordering appropriate accessories. Glass plates and spacers are available in heights of 22cm 32cm and 42cm. A wide selection of precision-machined combs are available. Compressible long-lasting silicone gasket on face of unit provides leak- proof seal when glass plates are clamped in place. Gel-Wrap casting system makes gel casting quick and easy for gel thicknesses of 0.75mm 1.0mm 1.5mm 2.0mm and 3.0mm. Bottom buffer reservoirs conveniently detaches from base of unit for ease in cleaning and disposal of buffer. Accessory anodized aluminum cooling plates can be ordered to clamp onto glass plates to evenly distribute the heat generated during electrophoresis. This system can be used for fluorescence based assays whose images can be captured on a scanner directly through optional borosilicate glass plates. APPLICATIONS SDS PAGE Native PAGE DNA Sequencing Isoelectric Focusing Large Format Dual Adjustable Vertical System for Nucleic Acid Applications LDASG-400-20NA Includes same as above except Choose from thinner combs and spacers charts Glass plates and spacers are not modified for use with Gel Wrap Gel Wrap gaskets are not included. Large Format Adjustable Vertical System for Nucleic Acid Applications LSG-400-20NA Includes same as above except Choose from thinner combs and spacers charts Glass plates and spacers are not modified for use with Gel Wrap Gel Wrap gaskets are not included. LDASG-400-20 shown with optional annodized aluminum cooling plate LSG-400-20 shown with optional annodized aluminum cooling plate SPECIFICATIONS Cat. of Gels Overall Dimensions W x D x H cm Glass Plate Dimensions W x L cm Spacer Comb Thickness Offered mm Recommended Buffer Reservoir Volume mls LSG-400-20 1 24 x 19 x 31 24 x 19 x 41 24 x 19 x 41 20 x 22 20 x 32 20 x 42 0.75 1.0 1.5 2.0 3.0 310-375 upper 150-350 lower LDASG-400-20 2 24 x 19 x 31 24 x 19 x 41 24 x 19 x 41 20 x 22 20 x 32 20 x 42 0.75 1.0 1.5 2.0 3.0 375-475 upper 150-350 lower LSG-400-20NA 1 24 x 19 x 31 24 x 19 x 41 24 x 19 x 41 20 x 22 20 x 32 20 x 42 0.2 0.4 310-375 upper 150-350 lower LDASG-400- 20NA 2 24 x 19 x 31 24 x 19 x 41 24 x 19 x 41 20 x 22 20 x 32 20 x 42 0.2 0.4 375-475 upper 150-350 lower p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 31 VERTICALS COMB OPTIONS THICKER material PTFE overall length 15.87cm Cat. of Teeth Tooth Depth mm Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG20-0708 8 19.05 0.75 17.7 95 SG20-0712 12 19.05 0.75 11 58 SG20-0717 17 19.05 0.75 6.63 35 SG20-0720 20 19.05 0.75 5.66 30 SG20-0728 28 17.45 0.75 4 22 SG20-0732 32 17.45 0.75 3.4 18 SG20-0735 35 17.45 0.75 2.9 14 SG20-1008 8 19.05 1.0 17.7 126 SG20-1012 12 19.05 1.0 11 78 SG20-1017 17 19.05 1.0 6.63 50 SG20-1020 20 19.05 1.0 5.66 40 SG20-1028 28 17.45 1.0 4 29 SG20-1032 32 17.45 1.0 3.4 24 SG20-1035 35 17.45 1.0 2.9 19 SG20-1508 8 19.05 1.5 17.7 190 SG20-1512 12 19.05 1.5 11 117 SG20-1517 17 19.05 1.5 6.63 71 SG20-1520 20 19.05 1.5 5.66 60 SG20-1528 28 17.45 1.5 4 43 SG20-1532 32 17.45 1.5 3.4 36 SG20-1535 35 17.45 1.5 2.9 29 SG20-2008 8 19.05 2.0 17.7 252 SG20-2012 12 19.05 2.0 11 156 SG20-2017 17 19.05 2.0 6.63 94 SG20-2020 20 19.05 2.0 5.66 80 SG20-2028 28 17.45 2.0 4 57 SG20-2032 32 17.45 2.0 3.4 48 SG20-2035 35 17.45 2.0 2.9 38 SG20-3008 8 19.05 3.0 17.7 378 SG20-3012 12 19.05 3.0 11 234 SG20-3017 17 19.05 3.0 6.63 140 SG20-3020 20 19.05 3.0 5.66 119 SG20-3028 28 17.45 3.0 4 86 SG20-3032 32 17.45 3.0 3.4 72 SG20-3035 35 17.45 3.0 2.9 56 THICKER GELS C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals large format glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished within a few minutes. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. Gel Wrap is available for gel thickness of 0.75mm 1.0mm 1.5mm 2.0mm and 3.0mm. See page 23. THINNER GELS For casting gels 0.2mm or 0.4mm thick we offer the Gel Slider cat. GSL-1001 pictured at right. Alternatively glass plates can be taped for gel formation. See page 77. Gel Casting Options SHARKTOOTH COMB OPTIONS THINNER for Nucleic Acid Separations Cat. Comb Type of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Well Volume l SK20-0201 casting 1 0.20 158.7 NA SK20-0234 sharktooth 34 0.20 4.5 3.3 SK20-0252 sharktooth 52 0.20 3.0 2.0 SK20-0270 sharktooth 70 0.20 2.25 1.4 SK20-0401 casting 1 0.40 158.7 NA SK20-0434 sharktooth 34 0.40 4.5 6.6 SK20-0452 sharktooth 52 0.40 3.0 4.0 SK20-0470 sharktooth 70 0.40 2.25 2.8 RECTANGULAR POLYCARBONATE COMB OPTIONS THINNER for Nucleic Acid Separations material Polycarbonate tooth depth 9.5mm overall length 15.87cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG20-0208 8 0.20 17.7 25.2 SG20-0212 12 0.20 11 15.7 SG20-0214 14 0.20 9 13 SG20-0216 16 0.20 7.6 10.8 SG20-0217 17 0.20 6.6 9.4 SG20-0220 20 0.20 5.6 8 SG20-0228 28 0.20 4 5.8 SG20-0232 32 0.20 4.3 4.8 SG20-0235 35 0.20 2.9 4.1 SG20-0408 8 0.40 17.7 50.5 SG20-0412 12 0.40 11 30.8 SG20-0414 14 0.40 9 25.5 SG20-0416 16 0.40 7.6 21 SG20-0417 17 0.40 6.6 18.8 SG20-0420 20 0.40 5.6 15.8 SG20-0428 28 0.40 4 11.3 SG20-0432 32 0.40 4.3 9 SG20-0435 35 0.40 2.9 8.3 Comb Options See product list or website for complete listing of accessories and ordering options ORDERING INFORMATION CAT. DESCRIPTION LARGE FORMAT ADJUSTABLE VERTICAL SYSTEMS LSG-400-20 Large Format Adjustable Vertical Kit for thicker gel applications. Glass plate dimensions 20cmw x 22cm - 42cmh capability. LSG-400-20NA Large Format Adjustable Vertical Kit for thinner gel applications. Glass plate dimensions 20cmw x 22cm - 42cmh capability. LDASG-400-20 Large Format Dual Adjustable Vertical Kit for thicker gel applications. Glass plate dimensions 20cmw x 22cm - 42cmh capability. LDASG-400-20NA Large Format Dual Adjustable Vertical Kit for thinner gel applications. Glass plate dimensions 20cmw x 22cm - 42cmh capability. EPS-600 Power Supply CE 5-600V 1-500mA EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. SPACER ORDERING INFORMATION THINNER - Cat. s for Nucleic Acid Separations - 2 side spacers Thickness mm 22cm Height 32cm Height 42cm Height 0.20 SGS-2020 SGS-2030 SGS-2040 0.40 SGS-4020 SGS-4030 SGS-4040 GEL WRAP SPACER ORDERING INFORMATION THICKER - Cat. s for Protein Separations - 2 side spacers with rounded corners Thickness mm 22cm Height 32cm Height 42cm Height 0.75 SGS-20-0720R SGS-20-0730R SGS-20-0740AR 1.0 SGS-20-1020R SGS-20-1030R SGS-20-1040AR 1.5 SGS-20-1520R SGS-20-1530R SGS-20-1540AR 2.0 SGS-20-2020R SGS-20-2030R SGS-20-2040AR 3.0 SGS-20-3020R SGS-20-3030R SGS-20-3040AR w w w. c b s s c i e n t i f i c . c o m32 VERTICALS COMB OPTIONS for Mega-Gel material PTFE tooth depth 10mm overall length 44.8cm Cat. Comb type of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol. Well l SG50-1050 microtitre 50 1.0 7.6 57 SG50-10100 microtitre 100 1.0 3.0 23 SG50-1550 microtitre 50 1.5 7.6 86 SG50-15100 microtitre 100 1.5 3.0 34 FEATURES High-throughput format allows up to 200 samples per run. To enhance screening productivity the system includes two 100 well combs that feature microtitre spacing for multi-channel pipettor loading. To further maximize throughput multiple amplification products can be loaded simultaneously or successively in the same well reference 1 page 1830 Gel-Wrap casting system makes gel casting quick and easy. See page 23. 0.2mm thick comb and spacer accessories are available for Microsatellite Mapping The glass plate sandwich includes a low fluorescence back plate making it ideal for fluorescence-based assays. This feature allows the bands to be visualized without the time-consuming process of removing the gel from the glass plate sandwich and staining. Ethidium bromide is added to the lower reservoir prior to electrophoresis and the low fluorescence glass allows direct UV visualization and photography of the results without gel removal. This adjustable system offers glass plate sizes of 22cm and 32cmh. Dual-sided apparatus has rotating base for easy loading and conserves valuable bench space. Mega-Gel System High Throughput Vertical C-DASG-400-50 Complete System Dual sided high-throughput adjustable height electrophoresis unit with guide rods and rotatingleveling base Platinum Electrodes 2 each 100 well combs 2 sets of Gel Wrap spacers 2 lengths of Gel Wrap gasket 2 sets of 50cmw x 22cmh Gel Wrap glass plates with low fluorescence back plate Safety covers with power leads White spring clamps Temperature strips Glass plate separators Conversion plate for running one gel at a time Mega-Gel gels can be cast using the Gel Wrap Casting System C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that seals the glass plate sandwich for gel casting quickly and easily. Set-up time for gel casting can be accomplished in less than one minute. Gel Wrap is designed to perform two functions simultaneously - it holds onto the back glass plate making it easy to handle and it seals the spacers. See page 23. Gel Casting References 1 Wang D. Shi J. Carlson S. R. Cregan P.R. Ward R. W. and B. W. Diers A. Low- Cost High-Throughput Polyacrylamide Gel Electrophoresis System for Genotyping with Microsatellite DNA Markers. Crop Science 4318281832 2003. APPLICATIONS Microsatellite Mapping Minisatellites Mutation Detection DNA Footprinting SDS-PAGE DNA Fingerprinting SSR genotyping for linkage construction Mega-Gel System High Throughput Vertical Unique size for optimal productivity SPECIFICATIONS Overall Dimensions W x D x H cm 60 x 34 x 44 Glass Plate Dimension Options W x L cm 50 x 22 50 x 32 Recommended Buffer Volume upper reservoirs - 500mleach lower reservoirs - 500mleach Total - 2 Liters Running Conditions 350VD105mAgel1.5 thick Recommended Power Supply Power Supply EPS-600 5-600V ORDERING INFORMATION CAT. DESCRIPTION MEGA-GEL ELECTROPHORESIS SYSTEM C-DASG-400-50 Mega-Gel System CE Complete System Accessories and Related Products SGS-50-1020R Spacer Set 1.0mm x 22cml SGS-50-1030R Spacer Set 1.0mm x 32cml SGS-50-1520R Spacer Set 1.5mm x 22cml SGS-50-1520R Spacer Set 1.5mm x 32cml SGP50-020R-DN Gel Wrap Glass Plate Set 50cmw x 22cml with Borosilicate back plate SGP50-030R-DN Gel Wrap Glass Plate Set 50cmw x 32cml with Borosilicate back plate SGP50-020R Gel Wrap Glass Plate Set 50cmw x 22cml stan- dard SGP50-030R Gel Wrap Glass Plate Set 50cmw x 32cml stan- dard SGE50-1020 Gel Wrap Gasket 1.0mm x 22cm SGE50-1030 Gel Wrap Gasket 1.0mm x 32cm SGE50-1520 Gel Wrap Gasket 1.5mm x 22cm SGE50-1530 Gel Wrap Gasket 1.5mm x 32cm EPS-600 Power Supply CE 5-600V 1-500mA Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. For thinner gels see website for mylar comb and spacer sets. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 33 VERTICALS DESCRIPTION The Pore Gradient Lipoprotein Electrophoresis System LPE is designed to resolve lipoprotein sub-classes of low density lipoproteins LDL and high density lipoproteins HDL based on particle size using non-denaturing pore gradient gel electrophoresis 1 2 3. Other applications include standard PAGE and short DNA sequencing runs. Temperature control 8C - 10C is accomplished using a cooling coil in the buffer chamber that is connected to an external refrigerated water bath. A built-in pump recirculates the buffer and helps keep the temperature constant. Up to four gels can be run simultaneously. Slots in a specially molded silicone gasket hold the gels in place in the upper reservoir. 3 gel slot plugs are included in the event the researcher would like to run less than 4 gels at a time. Glass Plates and Spacers Glass plates and spacers can be ordered for researchers that desire to pour their own gradient gels. Glass plates dimensions are 8.2cm wide by 8.2cm high cat. LPG-082. Spacers are 82mm high and have a thicknesses of 2.8mm cat. LPS-028. Sample Applicators Sample applicators can be ordered with a choice of 12 cat. LPE-SL12 13 cat. LPE-SL13 or 18 cat. LPE-SL18 wells. Sample applicators are inserted into the top of the pre-cast gels for sample loading and remain in place during the electrophoresis. LDL HDL Pore Gradient Gel Electrophoresis Results Composite gel 3mm thick PAA 2-31 demonstrating both LDL and HDL sub- classes. Sample volume 10l. Pre-run 120V for 60 minutes 15V for 15 minutes 70V for 20 minutes 125V for 24 hours 3000 volt hours. References 1 Rainwater David L. et al. Characterization of a composite gradient gels for the electrophoretic separation of lipoproteins. Journal of Lipid Research Volume 38 1997. 2 Rainwater David L. et al. Production of polyacrylamide gradient gels for the electrophoretic resolution of lipoproteins. Journal of Lipid Research Volume 33 1992. 3 Rainwater David L. Electrophoretic Separation of LDL and HDL Subclasses Methods in Molecular Biology Volume 110 Lipoprotein Protocols. Pore Gradient LPE System LPE-4003 Complete System Base unitlower buffer reservoir with cooling coil and external connections for refrigerated water bath 4 sample applicators with choice of 12 13 or 18 wells Upper reservoir with 4-slot molded silicone gasket Platinum Electrodes 3 gel slot plugs for molded silicone gasket. Plugs are necessary when running less than 4 gels at a time Safety cover with electrical interlock Buffer recycling pump Pore Gradient LPE Systems ORDERING INFORMATION CAT. DESCRIPTION PORE GRADIENT LPE SYSTEM LPE-4003 Pore Gradient LPE System CE Complete System LPE-4003-220V Pore Gradient LPE System CE 220V Complete System Related Products LPE-SL12 Sample Applicator 12 well LPE-SL13 Sample Applicator 13 well LPE-SL18 Sample Applicator 18 well EPS-300 X Power Supply CE 1-300V 1-500mA Resolve Lipoprotein Sub-Classes APPLICATIONS Resolution of Lipoprotein subclasses HDL and LDL Standard PAGE SPECIFICATIONS Overall Dimensions W x D x H cm 39 x 12 x 28 Glass Plate Dimensions W x L cm 8.2 x 8.2 Recommended Buffer Volume 3.5 Liters w w w. c b s s c i e n t i f i c . c o m34 BLOTTING Temperature controlled Tank Blotting Systems fit four 10cm x 10cm mini-gels FEATURES Tank Blotting System includes a cooling grid in base for temperature control during runs. Capability of transferring 4 gels simultaneously under identical temperature controlled buffer conditions Transfer cassette assembly consists of hinged molded polycarbonate panels and sponge pads. Stirring bar can be placed beneath the cassettes to allow buffer circulation even temperature distribution and heat exchange during electrophoresis. Fits four gels with precast cassette or glass plate dimensions of 10cm x 10cm. Plate Electrode Option EBU-204P The anode is made from a platinum- plated Titanium sheet the cathode is made of durable 351 stainless steel. Plate electrodes provide a more dense electrical field resulting in faster more efficient transfers. This option can speed up transfer of proteins over the whole spectrum of molecular weights. A high current power supply such as the EPX-250X must be used with plate electrodes. Mini-Blotting System 4-place EBU-204 EBU-204P Complete System Blotting chamber with room for spin bar beneath cassettes and internal cooling base for temperature controlled runs 2 platinum electrode panels EBU-204 or plate electrode option EBU-204P 4 transfer cassettes with 4 sponge pads 4 additional sponge pads Safety cover with power leads Tubing adapters SPECIFICATIONS Cat. Overall Dimensions L x W x H cm Recommended Buffer Volume mls Maximum of Gels Method of Temperature Control Fits Gel Cassette Glass Plate Size W x H cm Compatible C.B.S. Scientific Vertical Systems catalog number EBX-700 14 x 13 x 16 1200 no freezer blocks 850 2 freezer blocks 4 freezer blocks 10 x 10 10 x 8 DCX-700 QNX-700 QNC-700 MGV-302 MGV-402 MGV-102 MGV-202 EBU-204 EBU-204P 14 x 13 x 16 1200 4 cooling base 10 x 10 10 x 8 DCX-700 QNX-700 QNC-700 MGV-302 MGV-402 MGV-102 MGV-202 APPLICATIONS Western Blotting Southern Blotting Northern Blotting Mini-Blotting System 4-place EBX-700 Complete System Molded blotting chamber with spin bar corral 4 freezer blocks 2 platinum electrode panels 4 transfer cassettes with 4 sponge pads 4 additional sponge pads Safety cover with power leads Mini-Blotting Systems FEATURES Temperature control is achieved through the use of freezer blocks on both ends of the reservoir eliminating the need for an external cooling apparatus. Capability of transferring 4 gels simultaneously under identical temperature controlled buffer conditions Transfer cassette assembly consists of hinged molded polycarbonate panels and sponge pads. Color-coded components for correct polarity orientation. Stirring bar can be placed beneath the cassettes to allow buffer circulation even temperature distribution and heat exchange during electrophoresis. Fits four gels with precast cassette or glass plate dimensions of 10cm x 10cm. EBU-204 EBX-700EBU-204P p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 35 BLOTTING red side blotting paper gel foam pad black side membrane Compatible Products Include Mini-Verticals These blotters fit gels from our MGV-102 and MGV-302 single vertical Mini-Gel Systems. Quadra Mini-Vertical 4-place Cat. s QNX-700 QNC-700 Runs 4 10cm x 10cm SDS- PAGE gels at a time. This combo unit can also be used for blotting. Dual Cool Mini-Vertical 2-place Cat. DCX-700 Runs 2 10cm x 10cm SDS- PAGE gels at a time. This combo unit can also be used for blotting. Cassette Features and Transfer Assembly Hinged cassette with double latches assures equal contact on blotting stack and allows easy loading and removal of blot The cassette assembly consists of two hinged molded polycarbonate panels and one sponge pad between which the gel membrane and blotting paper are placed. See diagram below. Dual Mini-Verticals These blotters fit gels from our MGV-202 and MGV-402 Dual Mini-Vertical Systems. ORDERING INFORMATION CAT. DESCRIPTION MINI-BLOTTING SYSTEMS - FOR UP TO 10CM X 10CM BLOTS EBU-204 Mini-Blotting System 4 place with cooling base and platinum electrode panels fits gel cassetteglass plate dimensions 10cmw x 10cm h EBU-204P Mini-Blotting System 4 place with cooling base and plate electrodes fits gel cassetteglass plate dimensions 10cmw x 10cm h EBX-700 Mini-Blotting System 4 place with freezer blocks fits gel cassetteglass plate dimensions 10cmw x 10cm h Accessories and Replacement parts for EBU-204 or EBX-700 EBX-BC-700 Additional Blotting Cassette EBX-SP-700 Additional Sponge Pads package of 4 EPX-250 X High Current Power Supply CE 2-250V 10-2500mA EPS-300 X Power Supply CE 1-300V 1-500mA Recommended Power Supplies See page 55 for complete description. EPS-300 X Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 1-300V output current 1-500mA in 1mA steps EPX-250 X High Current Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 2-250V output current 10-2500mA in 1mA steps w w w. c b s s c i e n t i f i c . c o m36 BLOTTING Double-Wide Mini-Blotters Temperature controlled Blotting Systems for Double-Wide Mini-Gels Double-Wide Mini-Blotting System 2-place EBU-402 Complete System Buffer chamber with room for spin bar beneath cassettes and internal cooling base for temperature controlled runs 2 platinum electrode panels 1 blotting cassette with 2 sponge pads Safety cover with power leads Tubing adapters SPECIFICATIONS Blotting System Cat. EBU-402 EBU-102 Overall Dimensions L x W x H cm 24 x 9.5 x 16 19 x 11 x 28.5 Method of Temperature Control cooling base cooling base Maximum of Cassettes 2 3 Fits Gel CassetteGlass Plate Size W x H cm 20 x 10 16 x 22 Compatible C.B.S. Scientific Vertical Systems Cat. MGV-102-20 MGV-202-20 DCX-800 DCX-800C MGV-116-20 MGV-216-20 Power Settings depending on number and thickness of gels At 4C 26V current range 250-300mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours At 4C 60V current range 100-600mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours Buffer Volume 1 cassette 1.5 Liters 2 cassettes 1.4 Liters 1 cassette 4.2 Liters 2 cassettes 3.95 Liters 2 cassettes 3.7 Liters Recommended Power Supply EPS-300 X EPX-250 X ORDERING INFORMATION CAT. DESCRIPTION DOUBLE-WIDE BLOTTING SYSTEMS EBU-402 Double-Wide Blotting System CE Includes 1 cassette fits gel cassetteglass plate dimensions 20cmw x 10cm h EBU-102 Large Blotting System CE Includes 1 cassette fits gel cassetteglass plate dimensions 20cmw x 16cm h Related Products EBC-402 Additional Blotting Cassette fits EBU-402 EBS-402 Additional Sponge Pads set of 4 fits EBU-402 EBC-102 Additional Blotting Cassette fits EBU-102 EBS-102 Additional Sponge Pads set of 4 fits EBU-102 EPX-250 X High Current Power Supply CE 2-250V 10-2500mA EPS-300 X Power Supply CE 1-300V 1-500mA APPLICATIONS Western Blotting Southern Blotting Northern Blotting FEATURES Provides the capability of transferring 2 gels simultaneously under identical temperature controlled buffer conditions Fits 2 each 20cm x 10 cm gels. 16cm high Double-Wide gels must be run in the EBU-102 Blotter detailed description on page 38. Internal cooling chamber in base of blotter for precise temperature control. The compatible Double-Wide Electrophoresis Systems are described on pages 18-19. Recommended Power Supplies See page 55 for complete description. EPS-300 X Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 1-300V output current 1-500mA in 1mA steps EPX-250 X High Current Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 2-250V output current 10-2500mA in 1mA steps Large Blotting System EBU-102 See complete description on page 38. Double-Wide gels are rotated 90 to fit this dimension p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 37 BLOTTING Triple-Wide Mini-Blotting Systems 2-place EBU-302 EBU-362 Complete System Blotting chamber with room for spin bar beneath cassettes and internal cooling base for temperature controlled runs 2 platinum electrode panels 1 blotting cassette with 2 sponge pads Safety cover with power leads Tubing adapters Temperature controlled Blotting Systems for Triple-Wide Mini-Gels Triple-Wide Mini-Blotters SPECIFICATIONS Blotting System Cat. EBU-302 EBU-362 Overall Dimensions L x W x H cm 38 x 10 x 16 39 x 9 x 22 Method of Temperature Control cooling base cooling base Maximum of Cassettes 2 2 Fits Gel CassetteGlass Plate Size W x H cm 33 x 10 33 x 16 Compatible C.B.S. Scientific Vertical Systems Cat. MGV-102-33 MGV-202-33 MGV-116-33 MGV-216-33 Power Settings Depending on number and thickness of gels At 4C 26V current range 250-300mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours At 4C 60V current range 100-600mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours Buffer Volume 1 cassette 2 Liters 2 cassettes 1.9 Liters 1 cassette 3 Liters 2 cassettes 2.8 Liters Recommended Power Supply EPS-300 X EPX-250 X APPLICATIONS Western Blotting Southern Blotting Northern Blotting FEATURES Provides the capability of transferring 2 gels simultaneously under identical temperature controlled buffer conditions 33cm wide Blotters are available in 2 heights Internal cooling chamber in base of blotter for precise temperature control The compatible Dual Triple-Wide Electrophoresis Systems see pages 20-21 MGV-202-33 MGV-216-33 are specifically designed for the mass screening of up to 204 samples so that results from 2 microtitre plates cans be transferred simultaneously. ORDERING INFORMATION CAT. DESCRIPTION TRIPLE-WIDE BLOTTING SYSTEMS EBU-302 Triple-Wide Blotting System CE Includes 1 cassette fits gel cassetteglass plate dimensions 33cmw x 10cm h EBU-362 Triple-Wide Blotting System CE Includes 1 cassette fits gel cassetteglass plate dimensions 33cmw x 16cm h Related Products EBC-302 Additional Blotting Cassette fits EBU-302 EBS-302 Additional Sponge Pads set of 4 fits EBU-302 EBC-362 Additional Blotting Cassette fits EBU-362 EBS-362 Additional Sponge Pads set of 4 fits EBU-362 EPX-250 X High Current Power Supply CE 2-250V 10-2500mA EPS-300 X Power Supply CE 1-300V 1-500mA Recommended Power Supplies See page 55 for complete description. EPS-300 X Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 1-300V output current 1-500mA in 1mA steps EPX-250 X High Current Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 2-250V output current 10-2500mA in 1mA steps w w w. c b s s c i e n t i f i c . c o m38 BLOTTING Large Blotting Systems EBU-102 EBU-102P EBU-222 Complete System Blotting chamber with room for spin bar beneath cassettes and internal cooling base for temperature controlled runs 2 platinum electrode panels EBU- 102 EBU-222 or plate electrodes EBU-102P 1 blotting cassette with 2 sponge pads Safety cover with power leads Tubing adapters FEATURES These larger Tank Blotting Systems include a cooling grid in base for temperature control during runs. The EBU-102 and EBU-102P Blotting Systems can accommodate 3 gels of dimensions up to 16cm x 22cm. The EBU-222 Blotting System has the capability of transferring 2 gels simultaneously with dimensions up to 20cm x 20cm. Plate Electrode Option EBU-102P The anode is made from a platinum-plated Titanium sheet the cathode is made of durable 351 stainless steel. Plate electrodes provide a more dense electrical field resulting in faster more efficient transfers. This option can speed up transfer of proteins over the whole spectrum of molecular weights. Cassette assembly consists of hinged perforated panels and either 1 or 2 sponge pads. Color-coded components for correct polarity orientation. Stirring bar can be placed beneath the cassettes to allow buffer circulation and heat exchange during electrophoresis. Large Blotting Systems EBU-102 ORDERING INFORMATION CAT. DESCRIPTION LARGE TANK BLOTTING SYSTEMS EBU-102 Large Blotting System CE 3 place fits gel cassetteglass plate dimensions 16cmw x 22cm h EBU-102P Large Blotting System CE 3 place plate electrodes fits gel cassetteglass plate dimensions 16cmw x 22cm h EBU-222 Large Blotting System CE 2 place with cooling base fits gel cassetteglass plate dimensions 20cmw x 20cm h Accessories and Related Products EBC-102 Additional Blotting Cassette for EBU-102 EBS-102 Additional Sponge Pads set of 4 fits EBU-102 EBC-222 Additional Blotting Cassette for EBU-222 EBS-222 Additional Sponge Pads set of 4 fits EBU-222 EPX-250 X High Current Power Supply CE 2-250V 10-2500mA SPECIFICATIONS Cat. EBU-102 EBU-102P EBU-222 Overall Dimensions L x W x H cm 19 x 11 x 28.5 23.5 x 7.8 x 27.7 Temperature Control cooling base cooling base Maximum of Cassettes 3 2 Fits Gel CassetteGlass Plate Size W x H cm 16cm x 22cm 20cm x 20cm Compatible C.B.S. Scientific Vertical Systems cat. ASG-250 ASG-250-02 DASG-250 DASG-250-02 DSG-125-02 DSG-160-02 DSG-170-02 DSG-190-02 DSG-200-02 MGV-116-20 MGV-216-20 All SG C.B.S. Lite models except -400s DCX-900C LSG-400-20 LDASG-400-20 MGV-116-20 MGV-216-20 Power Settings depending on number and thickness of gels At 4C 60V current range 100-600mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours At 4C 60V current range 100-600mAmp for 18 hours. At room temperature 26V current range 400- 500mAmp for 18 hours Buffer Volume 1 cassette 3.77 Liters 2 cassettes 3.76 Liters 3 cassettes 3.77 Liters 1 cassette 2.95 Liters 2 cassettes 2.6 Liters Recommended Power Supply EPX-250 X EPX-250 X APPLICATIONS Western Blotting Southern Blotting Northern Blotting Double-Wide gels are rotated 90 to fit this dimension EBU-222 Temperature controlled Blotting Systems for larger gels EBU-102P p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 39 BLOTTING Semi-Dry Blotting Systems EBU-3000 EBU-4000 EBU-6000 Complete System Chamber with stainless steel cathode and platinum-coated titanium anode Interlocking power leads with safety-tips Mylar mask sheets Semi-Dry Blotting Systems Semi-Dry blotting technique is fast and minimizes buffer consumption APPLICATIONS Electro-Blotting Transfers Western Southern Northern FEATURES Semi-Dry Blotting Systems are available in 3 sizes The stainless steel cathode and platinum-coated titanium anode transfer gels reliably and quickly and are durable and corrosion-free. The upper electrode housing is separate from the base allowing it to be secured with hand screws at the desired height. This unique design feature enables several gels to be stacked and transferred simultaneously. The electrodes create a uniform electric field producing excellent transfers onto nitrocellulose supported nitrocellulose nylon charged nylon or activated paper substrates. Blotting is accomplished by layering transfer membrane and gel between buffer saturated blotting paper minimizing the amount of buffer required. To prevent drawing current away from the gel during a transfer the uncovered portion of the cathode is shielded with a Mylar mask. This shield insures that all applied current passes directly through the gel. Use of the mask will increase transfer efficiency and reduce transfer times up to 50. EBU-3000 Mylar is a registered trademark of Dupont Teijin Films. SPECIFICATIONS Semi-Dry Blotter Cat. Transfer Area Dimensions cm Overall Dimensions L x W x H cm Corresponding C.B.S. Scientific Vertical Units - Cat. s EBU-3000 34 x 12 40 x 18.5 x 5 DCX-800 DCX-800C MGV-102-20 MGV-202-20 MGV-102-33 MGV-202-33 EBU-4000 20 x 20 29.8 x 29.8 x 6.8 DCX-700 QNC-700 QNX-700 MGV-102 MGV-102 MGV-302 MGV-402 MGV-116-20 MGV-216-20 ASG-250 ASG-250-02 DASG-250 DASG-250-02 DSG-125-02 DSG-160-02 DSG-170-02 DSG-190-02 DSG-200-02 All SG C.B.S. Lite models except -400s DCX-900C EBU-6000 40 x 35 50 x 43.2 x 6.8 MGV-116-33 MGV-216-33 LSG-400-20 LDASG-400-20 SG-400-33 ORDERING INFORMATION CAT. DESCRIPTION SEMI-DRY BLOTTING SYSTEMS EBU-3000 Semi-Dry Blotting System 34cm x 12cm transfer area EBU-4000 Semi-Dry Blotting System 20cm x 20cm transfer area EBU-6000 Semi-Dry Blotting System 40cm x 35cm transfer area Related Products EPX-250 X High Current Power Supply CE 2-250V 10-2500mA EBU-4000 Recommended Power Supply See page 55 for complete description. EPX-250 X High Current Power Supply Universal input with timer. Input 100-240 VAC 50-60 Hz. Output voltage 2-250V output current 10-2500mA in 1mA steps w w w. c b s s c i e n t i f i c . c o m40 HORIZONTALS BaseMetrix Buffer and DNA Ladders BaseMetrix Rapid Electrophoresis Buffer for Agarose Gels Get results 3 to 4 times faster DESCRIPTION The 100 bp double-sranded DNA ladder shown at immediate right ranges from 100 bp to 1Kb in exact 100 bp increments in Type II loading buffer 2.5 wv Ficoll-400 0.01 wv each of tracking dyes Bromophenol blue and Xylene cyanol FF 5mM EDTA. The 1Kb or 1000 bp double-stranded DNA ladder shown on far right ranges from 1000 bp to 15000 bp in exact 1000 bp increments in the Type II loading buffer described above. STORAGE STABILITY DNA ready-to-load ladder mixes may be kept safely at room temperature for at least 2 months. For longer-term storage 4C is recommended. Ladders are guaranteed for 12 months from date of shipment when stored at 4C RECOMMENDATIONS FOR USE Typically use 5l of ready-to-load ladder for gel wells of 5 mm width. This translates to 15-20 ngband -- giving sharp easily visible and photographed bands. Use proportionally more or less for wider or narrower wells. Ready-to-load ladder mixes BaseMetrix DNA Ladders A 15 Ra B 1 C 2 B B B 1 C 2 R A 1 R A 15 minutes BaseMetrix Rapid Buffer B 1 hour TAE buffer Legend 6g of BaseMetrix 100bp 500bp and 1Kb DNA ladders were loaded into lanes 3 5 and 7 respectively. Both gels are 1 agarose. Figure 1 DESCRIPTION BaseMetrix Rapid Buffer allows agarose gel electrophoresis to be performed at much higher voltage without the heat and high current problems band distortion and melting of the agarose gel associated with TAE or TBE buffers. Typical current used with this rapid buffer can be up to 250V and resolution and results can be completed 3-4 times faster than a TAE or TBE buffer. Refer to figure 1 images below. This product is for in vitro research use only. ORDERING INFORMATION CAT. DESCRIPTION BASEMETRIX RAPID ELECTROPHORESIS BUFFER BMB-1000 BaseMetrix Rapid Electrophoresis Buffer 10X 1 liter BMB-0100 BaseMetrix Rapid Electrophoresis Buffer 10X trial size 100ml ORDERING INFORMATION CAT. DESCRIPTION BASEMETRIX DNA LADDERS BML-0100 BaseMetrix DNA Ladders 100 bp DNA ladder Ready-to-load 500l 32gml BML-1000 BaseMetrix DNA Ladders 1000 bp DNA ladder Ready-to-load 500l 32gml bp 1000 100 200 300 400 500 900 800 700 600 5000 10000 15000 4000 3000 2000 1000 100 bp 1000 bp bp Run Time Comparison p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 41 HORIZONTALS Mini-Fast Sub Fast compact and safe design Mini-Fast Sub MGU-303 Complete System UV transparent base with grooves for gel casting dams 2 comb slots and built-in storage compartment Platinum electrodes 2 gel casting dams 2 combs of your choice Safety cover with power leads FEATURES Run times are less than an hour. UV transparent base allows sample visualization simply by transferring unit to transilluminator. This uniplanar design has a buffer chamber only slightly longer than the gel itself minimizing the amount of buffer required - 50 ml of 0.5X TBE recommended. Maximum of 50ml of agarose is needed. Can use as little as 30ml for analytical runs with small sample volumes. 1 or 2 combs positions for a maximum of 40 sample throughput. Two precision-fit PVC dams snap snuggly into grooves for leak-free gel casting in gel bed. Since gels are cast in place they are ready to run without casting tray or gel handling. Less than 15 minute run time required for elution of DNA in dialysis bags. APPLICATIONS Separation identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides Electro-elution of DNA in dialysis bags COMB OPTIONS material PVC tooth depth 5.37mm overall length 8.8cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol. Well l MFS-1004 4 1.0 20.2 82 MFS-1008 8 1.0 8.88 36 MFS-1016 16 1.0 3.27 13 MFS-1020 20 1.0 3.05 12 MFS-2004 4 2.0 20.2 163 MFS-2008 8 2.0 8.88 72 MFS-2016 16 2.0 3.27 26 MFS-2020 20 2.0 3.05 24 Base Profile and Migration Distances 1 comb 1 pathlength of up to 6cm green arrow 2 combs 2 pathlengths of up to 2.6cm blue arrows storage compartment grooves for casting dams comb slots 2.6cm2.6cm 6.0cm ORDERING INFORMATION CAT. DESCRIPTION MINI-FAST SUB MGU-303 Mini-Fast Sub CE Complete System gel dimension 10cmw x 8cml Related Products MCD-300 Additional Casting Dams set of 2 EPS-300 X Power Supply CE 1-300V 1-500mA SPECIFICATIONS Horizontal System Cat. Overall Dimension W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Agarose Solution Required ml of Comb Slots PathlengthSlot cm Maximum of Samples MGU-303 14 x 17 x 8 10 x 8 50 38 2 2.6 40 Agarose required was calculated using a gel thickness of 0.5cm w w w. c b s s c i e n t i f i c . c o m42 HORIZONTALS FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. All gel trays are winged for easy handling. No more sticking fingers into slippery buffer to extract gel tray. 2 gel bed sizes to choose from 5.5cmw x 8.5cml 7.5cmw x 10cml Productivity option allows casting a second gel while the first is running. The addition of the external gel caster and additional gel tray allows the researcher to use time more efficiently. Tapered baffles allow easy in situ gel formation with or without gel tray. Run up to 32 samples per gel. Combs are printed with catalog number and thickness for easy identification. Choice of double-sided adjustable height combs gives researcher more options. Optional PTFE-coated aluminum combs for low-friction comb removal and accelerated well formation. Horizontal Mini-Gel Systems are available in four vivid colors green yellow blue or fluorescent pink. These color-coded horizontal systems pictured at right are recommended to differentiate exclusive use by a specific lab or for a specific purpose. For example pink color-coded mini-gels can be assigned for a specific use such as RNA ONLY. Mini-Horizontal Productivity Package Option GCMHU-102 GCMHU-202 Complete Package Electrophoresis unit with molded tapered baffles 2 UV transparent winged gel trays with 2 comb position slots UV transparent acrylic gel bed 2 dual-sided adjustable height combs Interlock safety cover with thread-on power leads Platinum electrode cassettes External gel caster with leveling base Productivity Package Option allows casting a second gel while the first is running for increased efficiency Mini-Horizontals APPLICATIONS Separation and identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides Standard Mini-Horizontal Kit MHU-102 MHU-202 Complete Package Electrophoresis unit with molded tapered baffles 1 UV transparent winged gel tray with 2 comb position slots UV transparent acrylic gel bed 1 dual-sided or standard adjustable height comb Interlock safety cover with thread-on power leads Platinum electrode cassettes Productivity Package GCMHU-102 pictured above Mini-Gel tray features 1 Etched migration ruler on base. 2 1mm calibrated gel anchors on all 4 corners. Each 1mm step adds 4.7ml MHU-102 or 7.4ml MHU-202 of agarose. 3 Two comb slots allows choice of one longer pathlength or two shorter pathlengths. 4 Winged for easy handling 1mm 6mm Gel tray agarose required was calculated using a gel thickness of 0.5cm SPECIFICATIONS Horizontal System Cat. Overall Dimension W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel Tray Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray Pathlength Slot cm Maximum of Samples GCMHU-102 MHU-102 8.5 x 18.5 x 13 5.7 x 8.8 150 MGT-122 5.2 x 8.8 23 2 5.0 32 GCMHU-202 MHU-202 10.5 x 20.5 x 13 7.7 x 10 250 MGT-222 7.2 x 10 36 2 5.5 32 Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 43 HORIZONTALS DUAL-SIDED COMB OPTIONS for 5.5cm wide GCMHU-102 and MHU-102 adjustable height material Polycarbonate or PTFE-coated aluminum tooth depth 6.35mm overall length 5.0cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MGC-10-0810 810 1.0 4.773.5 2317 MGC-10-1216 1216 1.0 2.61.6 128 MGC-15-0810 810 1.5 4.773.5 3425 MGC-15-1216 1216 1.5 2.61.6 1811 MGC-20-0810 810 2.0 4.773.5 4533 MGC-20-1216 1216 2.0 2.61.6 2415 DUAL-SIDED COMB OPTIONS for 7.5cm wide GCMHU-202 and MHU-202 adjustable height material Polycarbonate or PTFE-coated aluminum tooth depth 6.35mm overall length 7.0cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol. Well l MHC-10-1014 1014 1.0 5.53.5 2617 MHC-10-0816 816 1.0 7.32.8 3513 MHC-15-1014 1014 1.5 5.53.5 3825 MHC-15-0816 816 1.5 7.32.8 5219 MHC-20-1014 1014 2.0 5.53.5 5133 MHC-20-0816 816 2.0 7.32.8 6926 STANDARD COMB OPTIONS for 7.5cm wide for GCMHU-202 and MHU-202 adjustable height material Polycarbonate or PTFE-coated aluminum tooth depth 6.35mm overall length 7.0cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol. Well l MHC-1001 1 1.0 69.8 332 MHC-1003E 3 equal 1.0 22.2 106 MHC-1003M 2 marker 1.0 30.6 146 1.0 3.29 16 MHC-1005 5 1.0 12.6 60 MHC-1008 8 1.0 7.3 35 MHC-1010 10 1.0 5.5 26 MHC-1012 12 1.0 4.3 20 MHC-1014 14 1.0 3.5 17 MHC-1016 16 1.0 2.8 13 MHC-2001 1 2.0 69.8 665 MHC-2003E 3 equal 2.0 22.2 212 MHC-2003M 2 marker 2.0 30.6 292 2.0 3.29 31 MHC-2005 5 2.0 12.6 120 MHC-2008 8 2.0 7.3 69 MHC-2010 10 2.0 5.5 51 MHC-2012 12 2.0 4.3 41 MHC-2014 14 2.0 3.5 33 MHC-2016 16 2.0 2.8 26 STANDARD COMB OPTIONS for 5.5cm wide for GCMHU-102 and MHU-102 adjustable height material Polycarbonate or PTFE-coated aluminum tooth depth 6.35mm overall length 5.0cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol. Well l MGC-1001 1 1.0 49.4 235 MGC-1003E 3 equal 1.0 15.4 73 MGC-1003M 2 marker 1.0 21.6 103 1.0 2.98 14 MGC-1008 8 1.0 4.77 23 MGC-1010 10 1.0 3.5 17 MGC-1012 12 1.0 2.6 12 MGC-1014 14 1.0 2.0 9 MGC-1016 16 1.0 1.6 8 MGC-2001 1 2.0 49.4 462 MGC-2003E 3 equal 2.0 15.4 146 MGC-2003M 2 marker 2.0 21.6 206 2.0 2.98 28 MGC-2008 8 2.0 4.77 45 MGC-2010 10 2.0 3.5 33 MGC-2012 12 2.0 2.6 24 MGC-2014 14 2.0 2.0 18 MGC-2016 16 2.0 1.6 15 Color- Coded Option To specify color add the following color code to the end of the catalog -Y Yellow -P Purple -B Blue -PK Pink or -G Green. Note Mini-Horizontals are clear unless otherwise specified. ORDERING INFORMATION CAT. DESCRIPTION MINI-HORIZONTAL SYSTEMS GCMHU-102 Mini-Horizontal Productivity Package CE gel dimension 5.5cmw x 8.5cml GCMHU-202 Mini-Horizontal Productivity Package CE gel dimension 7.5cmw x 10cml MHU-102 Standard Mini-Horizontal Kit CE gel dimension 5.5cmw x 8.5cml MHU-202 Standard Mini-Horizontal Kit CE gel dimension 7.5cmw x 10cml Related Products MGT-122 Replacement Winged Gel Tray UVT for GCMHU-102 GCT-101 External Gel Caster fits 1 MGT-122 Winged Gel Tray MGT-222 Replacement Winged Gel Tray UVT for GC MHU-202 GCT-201 External Gel Caster fits 1 MGT-222 Winged Gel Tray EPS-300 X Power Supply CE 1-300V 1-500mA Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . w w w. c b s s c i e n t i f i c . c o m44 HORIZONTALS Midi-Horizontal Productivity Package Option GCMHU-402 GCMHU-502 GCMHU-602 Complete Package Electrophoresis unit with molded tapered baffles 2 UV transparent winged gel trays with multiple comb position slots UV transparent acrylic gel bed 2 adjustable height combs Interlock safety cover with thread-on power leads Platinum electrode cassettes External gel caster with leveling base Midi-Horizontals APPLICATIONS Separation and identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides WGT-400 WGT-500 WGT-600 11cm 4.0cm 3.5cm 3.0cm 10cm 16cm Gel Tray Profiles Standard Midi-Horizontal Kit MHU-402 MHU-502 MHU-602 Complete Package Electrophoresis unit with molded tapered baffles UV transparent winged gel tray with multiple comb position slots UV transparent acrylic gel bed 1 adjustable height comb Interlock safety cover with thread-on power leads Platinum electrode cassettes Productivity Package Option allows casting a second gel while the first is running for increased efficiency Productivity Package pictured above Gel tray agarose required was calculated using a gel thickness of 0.5cm SPECIFICATIONS Horizontal System Cat. Overall Dimensions W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel Trays Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray PathlengthSlot cm Maximum of Samples MHU-402 13 x 22.5 x 14 10.5 x 11 550 WGT-400 9.2 x 11 50 2 4.0 36 MHU-502 16.7 x 21.5 x 14 14 x 10 700 WGT-500 12.7 x 10 60 2 3.5 54 MHU-602 16.7 x 27.5 x 14 14 x 16 750 WGT-600 12.7 x 16 100 4 3.0 108 FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. All gel trays are winged for easy handling. No more sticking fingers into slippery buffer to extract gel tray. 3 gel bed sizes to choose from 10.5cmw x 11cml 14cmw x 10cml 14cmw x 16cml Productivity option allows casting a second gel while the first is running. The addition of the external gel caster and additional gel tray allows the researcher to use time more efficiently. The MHU-602 Midi-Horizontal runs up to a full microtitre plate 108 samples per gel. Tapered baffles allow easy in situ gel formation with or without gel tray. Combs are printed with catalog number and thickness for easy identification. Choice of double-sided adjustable height combs gives researcher more options. Optional PTFE-coated aluminum combs for low-friction comb removal and accelerated well formation. Horizontal Midi-Gels are available in four vivid colors green yellow blue or fluorescent pink. These color-coded horizontal systems are recommended to differentiate exclusive use by a specific lab or for a specific purpose. Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 45 HORIZONTALS COMB OPTIONS 10.5cm wide for MHU-402 adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 8.73cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l MBU-1005 5 1.0 16.2 153 MBU-1010 10 1.0 7.47 71 MBU-1012 12 1.0 5.83 56 MBU-1016 16 1.0 3.86 37 MBU-1018 18 1.0 3.37 32 MBU-1505 5 1.5 16.2 231 MBU-1510 10 1.5 7.47 106 MBU-1512 12 1.5 5.83 83 MBU-1516 16 1.5 3.86 55 MBU-1518 18 1.5 16.2 48 Color- Coded Option To specify color add the following color code to the end of the catalog -Y Yellow -P Purple -PK Pink or -G Green. Note Midi-Horizontals are blue unless otherwise specified. Multi-channel pipettor compatible. Refer to microtitre loading diagram on page 51 for the SGC14 comb series. Maximum loading volume is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . ORDERING INFORMATION CAT. DESCRIPTION MIDI-HORIZONTAL SYSTEMS CGMHU-402 Midi-Horizontal Productivity Package Option CE gel bed dimensions 10.5cmw x 11cml CGMHU-502 Midi-Horizontal Productivity Package Option CE gel bed dimensions 14cmw x 10cml CGMHU-602 Midi-Horizontal Productivity Package Option CE gel bed dimensions 14cmw x 16cml MHU-402 Standard Midi-Horizontal Kit CE gel bed dimensions 10.5cmw x 11cml MHU-502 Standard Midi-Horizontal Kit CE gel bed dimensions 14cmw x 10cml MHU-602 Standard Midi-Horizontal Kit CE gel bed dimensions 14cmw x 16cml Related Products AGCT-16520 Continuously Adjustable Gel Caster fits gel trays 14cmw and from 8-20cml. Fits all 3 sizes of Midi-Horizontal Gel Trays. GCT10-111 External Gel Caster fits 1 MGT-400 Gel Tray GCT10-112 External Gel Caster fits 2 MGT-400 Gel Trays GCT14-101 External Gel Caster fits 1 MGT-500 Gel Tray GCT14-102 External Gel Caster fits 2 MGT-500 Gel Trays GCT14-161 External Gel Caster fits 1 MGT-600 Gel Tray GCT14-162 External Gel Caster fits 2 MGT-600 Gel Trays WGT-400 Replacement Gel Tray UVT for GCMHU-402 WGT-500 Replacement Gel Tray UVT for GC MHU-502 WGT-600 Replacement Gel Tray UVT for GC MHU-602 EPS-300 X Power Supply CE 1-300V 1-500mA COMB OPTIONS 14cm wide for MHU-502 MHU-602 adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 12.06cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC14-1010 10 1.0 9.2 88 SGC14-1012 12 1.0 7.8 74 SGC14-1013 13 1.0 6.6 63 SGC14-1016 16 1.0 5.3 51 SGC14-1020 20 1.0 4.5 43 SGC14-1026 26 1.0 3.1 30 SGC14-1027 27 1.0 2.93 28 SGC14-1510 10 1.5 9.2 131 SGC14-1512 12 1.5 7.8 111 SGC14-1513 13 1.5 6.6 94 SGC14-1516 16 1.5 5.3 76 SGC14-1520 20 1.5 4.5 64 SGC14-1526 26 1.5 3.1 44 SGC14-1527 27 1.5 2.93 42 SGC14-2010 10 2.0 9.2 175 SGC14-2012 12 2.0 7.8 149 SGC14-2013 13 2.0 6.6 126 SGC14-2016 16 2.0 5.3 101 SGC14-2020 20 2.0 4.5 86 SGC14-2026 26 2.0 3.1 59 SGC14-2027 27 2.0 2.93 56 SGC14-3010 10 3.0 9.2 263 SGC14-3012 12 3.0 7.8 223 SGC14-3013 13 3.0 6.6 189 SGC14-3016 16 3.0 5.3 151 SGC14-3020 20 3.0 4.5 128 SGC14-3026 26 3.0 3.1 89 SGC14-3027 27 3.0 2.93 83 w w w. c b s s c i e n t i f i c . c o m46 HORIZONTALS Flexicast Horizontal 4 in 1 WHSE-014 Complete System Flexicast Horizontal Unit with gel bed dimensions of 14cm w x 20cm l internal cooling chamber buffer circulation ports interlock- ing safety cover with thread-on power leads. 4 different length winged gels trays 14cmw x 8cm 14cmw x 12cm 14cmw x 16cm 14cmw x 20cm shown in caster Continuously Adjustable Gel Caster Platinum electrode cassettes 2 adjustable height polycarbonate combs WHSE-014 8cml 12cml 16cml 20cml Flexicast Horizontal Systems One Unit multiple gel tray lengths and Adjustable Gel Caster Continuously Adjustable Gel Caster Continuously Adjustable Caster cat. ACGT-16520 is easy to use and fits all 4 gel trays included in the Flexicast Systems. This Caster is adaptable to any gel tray between 16cmw and from 8-20cml. The gel tray friction fits between 2 rubber gaskets using a finger cam. See page 54 for complete description. 1. Place gel tray against stationary gasket and with finger cam in horizontal position slide gate to meet gel tray. 2. Friction fit gel tray into place and move finger cam into vertical position. Pour gel. 3. After agarose has cooled re- turn finger cam to horizontal position and slide gate away. APPLICATIONS Separation and identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides Flexicast Horizontal 3 in 1 WMHE-600 Complete System Flexicast Midi-Horizontal Unit with gel bed dimensions of 14cm w x 16cm l interlocking safety cover with thread-on power leads. 3 different length winged gels trays 14cmw x 8cm 14cmw x 12cm 14cmw x 16cm Continuously Adjustable Gel Caster Platinum electrode cassettes 2 adjustable height polycarbonate combs 8cml 12cml 16cml WMHE-600 FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. Gel Tray Specifications 1 All gel trays are winged for easy handling - no more sticking fingers into slippery buffer to extract gel tray. 2 Made from sturdy 6.4mm thick UV transparent acrylic. 3 Grooves in gel tray corners create agarose anchors to prevent gel flotation during electrophoresis. 4 Gel tray configuration allows single run or multiple simultaneous short runs. See Gel Tray Profiles. Save on agarose expense by selecting the appropriate length gel tray for the application desired. Continuously Adjustable Caster is easy to use and adaptable to any gel tray between 16cmw and from 8-20cml. The gel tray friction fits between 2 rubber gaskets using a finger cam. Combs are printed with catalog and thickness for easy identification. Adjustable height polycarbonate standard with unit or PTFE-coated aluminum combs are available in many configurations with or without markers. PTFE-coated combs allow faster well formation as no wells are lost when using low percentage or low melt agarose. Buffer recirculation ports allow maintenance of uniform pH and ionic strength during long runs in WHSE-014. Coolant can be recirculated beneath gel bed to provide constant temperature runs in the 4-in-1 WHSE-014 Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 47 HORIZONTALS Gel tray agarose required was calculated using a gel thickness of 0.5cm WGT-1408 WGT-1412 WGT-1416 WGT-1420 8cm 2.6cm 3.0cm 3.0cm 2.6cm 12cm 20cm 16cm Gel Tray Profiles ORDERING INFORMATION CAT. DESCRIPTION FLEXICAST SYSTEMS WMHE-600 Flexicast Midi-Horizontal CE maximum gel bed dimensions 14cmw x 16cml WHSE-014 Flexicast Horizontal CE maximum gel bed dimensions 14cmw x 20cml Related Products AGCT-16520 Additional Continuously Adjustable Gel Caster fits gel trays 16cmw and from 8-20cml WGT-1408 Replacement Winged Gel Tray UVT 14cmw x 8cml WGT-1412 Replacement Winged Gel Tray UVT 14cmw x 12cml WGT-1416 Replacement Winged Gel Tray UVT 14cmw x 16cml WGT-1420 Replacement Winged Gel Tray UVT 14cmw x 20cml EPS-300 X Power Supply CE 1-300V 1-500mA Multi-channel pipettor compatible. Refer to microtitre loading diagram on page 51. Maximum loading volume is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . SPECIFICATIONS Horizontal System Cat. Overall Dimensions W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel Trays Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray Pathlength Slot cm Maximum of Samples WMHE-600 16.7 x 27.5 x 14 14 x 16 750 WGT-1408 WGT-1412 WGT-1416 12.7 x 8 12.7 x 12 12.7 x 16 50 75 100 2 3 4 2.6 3.0 2.6 54 81 108 WHSE-014 15 x 38 x 10 14 x 20 1600 WGT-1408 WGT-1412 WGT-1416 WGT-1420 12.7 x 8 12.7 x 12 12.7 x 16 12.7 x 20 50 75 100 127 2 3 4 6 2.6 3.0 3.0 2.6 54 81 108 162 COMB OPTIONS 14cm wide adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 12.06cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC14-1010 10 1.0 9.2 88 SGC14-1012 12 1.0 7.8 74 SGC14-1013 13 1.0 6.6 63 SGC14-1016 16 1.0 5.3 51 SGC14-1020 20 1.0 4.5 43 SGC14-1026 26 1.0 3.1 30 SGC14-1027 27 1.0 2.93 28 SGC14-1510 10 1.5 9.2 131 SGC14-1512 12 1.5 7.8 111 SGC14-1513 13 1.5 6.6 94 SGC14-1516 16 1.5 5.3 76 SGC14-1520 20 1.5 4.5 64 SGC14-1526 26 1.5 3.1 44 SGC14-1527 27 1.5 2.93 42 SGC14-2010 10 2.0 9.2 175 SGC14-2012 12 2.0 7.8 149 SGC14-2013 13 2.0 6.6 126 SGC14-2016 16 2.0 5.3 101 SGC14-2020 20 2.0 4.5 86 SGC14-2026 26 2.0 3.1 59 SGC14-2027 27 2.0 2.93 56 SGC14-3010 10 3.0 9.2 263 SGC14-3012 12 3.0 7.8 223 SGC14-3013 13 3.0 6.6 189 SGC14-3016 16 3.0 5.3 151 SGC14-3020 20 3.0 4.5 128 SGC14-3026 26 3.0 3.1 89 SGC14-3027 27 3.0 2.93 83 w w w. c b s s c i e n t i f i c . c o m48 HORIZONTALS Standard SubmarineHorizontal Systems HSU-014 HSU-020 HSU-030 HSU-040 Complete System Electrophoresis unit with cooling chamber Platinum electrode cassettes UV transparent winged gel tray Tapered baffles for gel formation 1 adjustable height comb Buffer recirculation ports Leveling base with bubble level Interlock safety cover with thread-on power leads Horizontal Systems 4 sizes with 3 options for gel casting APPLICATIONS Separation and identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides Horizontal Productivity Package Option GCHSU-014 GCHSU-020 GCHSU-030 GCHSU-040 Complete System Electrophoresis unit with cooling chamber Platinum electrode cassettes External Gel Caster one place 2 UV transparent winged gel trays Tapered baffles for gel formation 2 adjustable height combs Buffer recirculation ports Leveling base with bubble level Interlock safety cover with thread-on power leads Gel tray agarose required was calculated using a gel thickness of 0.5cm Gel Tray Profiles WGT-020 WGT-030 WGT-040 20cm 20cm 30cm 40cm WGT-0142.6cm 2.6cm 2.7cm 2.7cm SPECIFICATIONS Horizontal System Cat. Overall Dimensions W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel tray Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray Pathlength Slot cm Maximum of Samples GCHSU-014 HSU-014 15 x 38 x 10 14 x 20 1600 WGT-014 12.7 x 20 127 6 2.6 162 GCHSU-020 HSU-020 21 x 43 x10 20 x 20 1850 WGT-020 18.7 x 20 187 6 2.6 240 GCHSU-030 HSU-030 21 x 53 x 10 20 x 30 1950 WGT-030 18.7 x 30 280 9 2.7 360 GCHSU-040 HSU-040 21 x 63 x 10 20 x 40 2050 WGT-040 18.7 x 40 374 12 2.7 480 FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. Four sizes of SubmarineHorizontal Systems in standard kits or with the Productivity Package Option Gel Tray Specifications 1 All gel trays are winged for easy handling -- no more sticking fingers into slippery buffer to extract gel tray. 2 Made from sturdy 6.4mm thick UV transparent acrylic. 3 Grooves in gel tray corners create agarose anchors to prevent gel flotation during electrophoresis. 4 Gel tray configuration allows single run or multiple simultaneous short runs. See Gel Tray Profiles. 3 options for gel casting as shown on page 49. Adjustable height polycarbonate or PTFE-coated aluminum combs are available in many configurations with or without markers. PTFE-coated combs allow faster well formation and no wells are lost when using low percentage or low melt agarose. Buffer recirculation ports allow maintenance of uniform pH and ionic strength and reduce frequency of buffer changes necessary during long runs. Tight-fitting safety cover reduces condensation during electrophoresis. Coolant can be recirculated beneath gel bed to provide constant temperature runs. Combs are printed with catalog and thickness for easy identification. Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 49 HORIZONTALS 1 In Situ Tapered Baffle System Simply slide baffles into grooves next to gel bed 1. Baffles rest friction tight against the gel tray or gel bed if no tray is used. After the gel has been poured and has solidified simply slide the baffles out of their slots. Gel Casting Options 2 External Gel Casters Pre- cast your agarose gels for future use in an easy to use and easy to store fashion. These support trays have leveling bases and are lined with soft closed cell neoprene to provide firm yet supple support for casting gels without leakage. 1 or 2-place casters are available for every size. Order from GCT series of Horizontal Gel Casters. 1 3 Continuously Adjustable Gel Caster See description on page 54. COMB OPTIONS 20cm wide adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 17.8cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC20-1012 12 1.0 12.8 123 SGC20-1020 20 1.0 6.7 65 SGC20-1030 30 1.0 4.5 43 SGC20-1040 40 1.0 3 28 SGC20-1512 12 1.5 12.8 183 SGC20-1520 20 1.5 6.7 97 SGC20-1530 30 1.5 4.5 64 SGC20-1540 40 1.5 3 42 SGC20-2012 12 2.0 12.8 244 SGC20-2020 20 2.0 6.7 129 SGC20-2030 30 2.0 4.5 86 SGC20-2040 40 2.0 3 56 SGC20-3012 12 3.0 12.8 365 SGC20-3020 20 3.0 6.7 194 SGC20-3030 30 3.0 4.5 128 SGC20-3040 40 3.0 3 85 ORDERING INFORMATION CAT. DESCRIPTION HORIZONTALSUBMARINE SYSTEMS GCHSU-014 HorizontalSubmarine Productivity Package CE gel bed dimensions 14cmw x 20cml GCHSU-020 HorizontalSubmarine Productivity Package CE gel bed dimensions 20cmw x 20cml GCHSU-030 HorizontalSubmarine Productivity Package CE gel bed dimensions 20cmw x 30cml GCHSU-040 HorizontalSubmarine Productivity Package CE gel bed dimensions 20cmw x 40cml HSU-014 Standard HorizontalSubmarine System CE gel bed dimensions 14cmw x 20cml HSU-020 Standard HorizontalSubmarine System CE gel bed dimensions 20cmw x 20cml HSU-030 Standard HorizontalSubmarine System CE gel bed dimensions 20cmw x 30cml HSU-040 Standard HorizontalSubmarine System CE gel bed dimensions 20cmw x 40cml Related Products AGCT-16520 Additional Continuously Adjustable Gel Caster fits gel trays 14cmw and from 5-20cml AGCT-21826 Additional Continuously Adjustable Gel Caster fits gel trays 21cmw and from 8-26cml AGCT-27940 Additional Continuously Adjustable Gel Caster fits gel trays 27cmw and from 9-40cml GCT-14-201 External Gel Caster fits 1 WGT-014 Gel Tray GCT-14-202 External Gel Caster fits 2 WGT-014 Gel Trays GCT-20-102 External Gel Caster fits 1 WGT-020 Gel Tray GCT-20-202 External Gel Caster fits 2 WGT-020 Gel Trays GCT-20-301 External Gel Caster fits 1 WGT-030 Gel Tray GCT-20-302 External Gel Caster fits 2 WGT-030 Gel Trays GCT-20-401 External Gel Caster fits 1 WGT-040 Gel Tray GCT-20-402 External Gel Caster fits 2 WGT-040 Gel Trays WGT-014 Replacement Gel Tray UVT 14cmw x 20cml WGT-020 Replacement Gel Tray UVT 20cmw x 20cml WGT-030 Replacement Gel Tray UVT 20cmw x 30cml WGT-040 Replacement Gel Tray UVT 20cmw x 40cml EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 Power Supply CE 5-600V 1-500mA Recommended for HSU-040 and GCHSU-040 Multi-channel pipettor compatible. Refer to microtitre loading diagram on page 51 for the SGC14 comb series. Maximum loading volume for is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . COMB OPTIONS 14cm wide adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 12.06cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC14-1010 10 1.0 9.2 88 SGC14-1012 12 1.0 7.8 74 SGC14-1013 13 1.0 6.6 63 SGC14-1016 16 1.0 5.3 51 SGC14-1020 20 1.0 4.5 43 SGC14-1026 26 1.0 3.1 30 SGC14-1027 27 1.0 2.93 28 SGC14-1510 10 1.5 9.2 131 SGC14-1512 12 1.5 7.8 111 SGC14-1513 13 1.5 6.6 94 SGC14-1516 16 1.5 5.3 76 SGC14-1520 20 1.5 4.5 64 SGC14-1526 26 1.5 3.1 44 SGC14-1527 27 1.5 2.93 42 SGC14-2010 10 2.0 9.2 175 SGC14-2012 12 2.0 7.8 149 SGC14-2013 13 2.0 6.6 126 SGC14-2016 16 2.0 5.3 101 SGC14-2020 20 2.0 4.5 86 SGC14-2026 26 2.0 3.1 59 SGC14-2027 27 2.0 2.93 56 SGC14-3010 10 3.0 9.2 263 SGC14-3012 12 3.0 7.8 223 SGC14-3013 13 3.0 6.6 189 SGC14-3016 16 3.0 5.3 151 SGC14-3020 20 3.0 4.5 128 SGC14-3026 26 3.0 3.1 89 SGC14-3027 27 3.0 2.93 83 w w w. c b s s c i e n t i f i c . c o m50 HORIZONTALS Econo-Sub Productivity Packages GCHSE-014 GCHSE-020 GCHSE-030 GCHSE-040 Complete System Electrophoresis unit with cooling chamber Platinum electrode cassettes External Gel Caster one-place 2 UV winged transparent gel trays 2 adjustable height combs Buffer recirculation ports Leveling base with bubble level Interlock safety cover with thread-on power leads Gel Casting Options 1 External Gel Caster Pre- cast your agarose gels for future use in an easy to use and easy to store fashion. These support trays have leveling bases and are lined with soft closed cell neoprene to provide firm yet supple support for casting gels without leakage. 1 or 2-place casters are available for every size. Order from GCT series of Horizontal Gel Casters. 2 Continuously Adjustable Gel Caster These continuously adjustable horizontal gel casters fit a wide range of gel trays and are easy to use. The gel tray is sandwiched between 2 rubber gaskets using an adjustable gate that can be locked into place using finger cams. See page 54. APPLICATIONS Separation and identification of DNA fragments RNA fragments PCR products and synthetic oligonucleotides Econo-Sub Productivity Packages Cast a second gel while the first is running for increased productivity and efficiency SPECIFICATIONS Horizontal System Cat. Overall Dimensions W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel tray Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray Pathlength Slot cm Maximum of Samples GCHSE-014 15 x 38 x 10 14 x 20 1600 WGT-014 12.7 x 20 127 6 2.6 162 GCHSE-020 21 x 43 x10 20 x 20 1850 WGT-020 18.7 x 20 187 6 2.6 240 GCHSE-030 21 x 53 x 10 20 x 30 1950 WGT-030 18.7 x 30 280 9 2.7 360 GCHSE-040 21 x 63 x 10 20 x 40 2050 WGT-040 18.7 x 40 374 12 2.7 480 Gel tray agarose required was calculated using a gel thickness of 0.5cm Gel Tray Profiles WGT-020 WGT-030 WGT-040 20cm 20cm 30cm 40cm WGT-0142.6cm 2.6cm 2.7cm 2.7cm FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. Four sizes of Econo-Sub Productivity Packages are offered with the following gel bed sizes 14cmw x 20cml 20cmw x 20cml 20cmw x 30cml 20cmw x 40cml Gel Tray Specifications 1 All gel trays are winged for easy handling -- no more sticking fingers into slippery buffer to extract gel tray. 2 Made from sturdy 6.4mm thick UV transparent acrylic. 3 Grooves in gel tray corners create agarose anchors to prevent gel flotation during electrophoresis. 4 Gel tray configuration allows single run or multiple simultaneous short runs. See Gel Tray Profiles. 2 options for gel casting as shown above right. Adjustable height polycarbonate or PTFE-coated aluminum combs are available in many configurations with or without markers. PTFE-coated combs allow faster well formation and no wells are lost when using low percentage or low melt agarose. Buffer recirculation ports allow maintenance of uniform pH and ionic strength and reduce frequency of buffer changes necessary during long runs. Tight-fitting safety cover reduces condensation during electrophoresis. Coolant can be recirculated beneath gel bed to provide constant temperature runs. Combs are printed with catalog and thickness for easy identification. Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 51 HORIZONTALS COMB OPTIONS 14cm wide for 3mm thickness see comb chart on page 49 adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 12.06cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC14-1010 10 1.0 9.2 88 SGC14-1012 12 1.0 7.8 74 SGC14-1013 13 1.0 6.6 63 SGC14-1016 16 1.0 5.3 51 SGC14-1020 20 1.0 4.5 43 SGC14-1026 26 1.0 3.1 30 SGC14-1027 27 1.0 2.93 28 SGC14-1510 10 1.5 9.2 131 SGC14-1512 12 1.5 7.8 111 SGC14-1513 13 1.5 6.6 94 SGC14-1516 16 1.5 5.3 76 SGC14-1520 20 1.5 4.5 64 SGC14-1526 26 1.5 3.1 44 SGC14-1527 27 1.5 2.93 42 SGC14-2010 10 2.0 9.2 175 SGC14-2012 12 2.0 7.8 149 SGC14-2013 13 2.0 6.6 126 SGC14-2016 16 2.0 5.3 101 SGC14-2020 20 2.0 4.5 86 SGC14-2026 26 2.0 3.1 59 SGC14-2027 27 2.0 2.93 56 COMB OPTIONS 20cm wide adjustable height printed with catalog and thickness material Polycarbonate or PTFE-coated aluminum tooth depth 12.7mm overall length 17.8cm Cat. of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC20-1012 12 1.0 12.8 123 SGC20-1020 20 1.0 6.7 65 SGC20-1030 30 1.0 4.5 43 SGC20-1040 40 1.0 3 28 SGC20-1512 12 1.5 12.8 183 SGC20-1520 20 1.5 6.7 97 SGC20-1530 30 1.5 4.5 64 SGC20-1540 40 1.5 3 42 SGC20-2012 12 2.0 12.8 244 SGC20-2020 20 2.0 6.7 129 SGC20-2030 30 2.0 4.5 86 SGC20-2040 40 2.0 3 56 SGC20-3012 12 3.0 12.8 365 SGC20-3020 20 3.0 6.7 194 SGC20-3030 30 3.0 4.5 128 SGC20-3040 40 3.0 3 85 Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . Possible loading strategies for microtitre compatible combs are listed below M marker lane is indicated with either a solid blue or red rectangle 12 or 8 number of wells loading capability for microtitre compatible spacing Microtitre Comb Profiles SGC14-XX27 M-8-8-M-8-M M-12-M-12-M M-8-M-M-8-M M-M-8-8-M-M M-12-M-12-M-12-M ORDERING INFORMATION CAT. DESCRIPTION ECONO-SUB PRODUCTIVITY PACKAGES GCHSE-014 Econo-Sub Productivity Package CE gel bed dimensions 14cmw x 20cml GCHSE-020 Econo-Sub Productivity Package CE gel bed dimensions 20cmw x 20cml GCHSE-030 Econo-Sub Productivity Package CE gel bed dimensions 20cmw x 30cml GCHSE-040 Econo-Sub Productivity Package CE gel bed dimensions 20cmw x 40cml Related Products AGCT-16520 Continuously Adjustable Gel Caster fits gel trays 14cmw and from 5-20cml AGCT-21826 Continuously Adjustable Gel Caster fits gel trays 21cmw and from 8-26cml AGCT-27940 Continuously Adjustable Gel Caster fits gel trays 27cmw and from 9-40cml GCT-14-201 External Gel Caster fits one 14cmw x 20cml tray GCT-14-202 External Gel Caster fits two 14cmw x 20cml trays GCT-20-102 External Gel Caster fits one 20cmw x 20cml tray GCT-20-202 External Gel Caster fits two 20cmw x 20cml trays GCT-20-301 External Gel Caster fits one 20cmw x 30cml tray GCT-20-302 External Gel Caster fits two 20cmw x 30cml trays GCT-20-401 External Gel Caster fits one 20cmw x 40cml tray GCT-20-402 External Gel Caster fits two 20cmw x 40cml trays WGT-014 Replacement Gel Tray UVT 14cmw x 20cml WGT-020 Replacement Gel Tray UVT 20cmw x 20cml WGT-030 Replacement Gel Tray UVT 20cmw x 30cml WGT-040 Replacement Gel Tray UVT 20cmw x 40cml EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 High Current Power Supply CE 5-600V 1-500mA recommended for GCHSE-040 SGC20-XX20 SGC20-XX40 for 3mm thickness see comb chart on page 49 w w w. c b s s c i e n t i f i c . c o m52 HORIZONTALS High-Throughput Submarine HSU-2626 APPLICATIONS Rapid screening of cloning products Rapid screening of restriction fragments Rapid screening of synthetic oligos Rapid screening of PCR products High-Throughput System HSU-2614 HSU-2626 HSU-2640 Complete System Electrophoresis unit with cooling chamber Platinum electrode cassettes UV transparent winged gel tray Tapered baffles for gel formation 4 adjustable height combs with aluminum backing Buffer recirculation ports Leveling base with bubble level Interlock safety cover with thread-on power leads COMB OPTIONS 26cm wide adjustable height material Polycarbonatealuminum backing or PTFE-coated aluminum overall length 24.7cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SGC26-0725 10 25 0.75 7.1 40 SGC26-0726 12 26 0.75 7 47 SGC26-0751 10 51 0.75 3.5 19 SGC26-07102 10 102 0.75 1.45 8 SGC26-1025 10 25 1.0 7.1 53 SGC26-1026 12 26 1.0 7 63 SGC26-1051 10 51 1.0 3.5 26 SGC26-10102 10 102 1.0 1.45 11 SGC26-1525 10 25 1.5 7.1 80 SGC26-1526 12 26 1.5 7 95 SGC26-1551 10 51 1.5 3.5 39 SGC26-15102 10 102 1.5 1.45 16 SGC26-2025 10 25 2.0 7.1 107 SGC26-2026 12 26 2.0 7 126 SGC26-2051 10 51 2.0 3.5 53 SGC26-20102 10 102 2.0 1.45 22 SGC26-3025 10 25 3.0 7.1 160 SGC26-3026 12 26 3.0 7 190 SGC26-3051 10 51 3.0 3.5 78 SGC26-30102 10 102 3.0 1.45 32 High-Throughput Systems Run up to 816 samples simultaneously with these versatile units Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. To specify PTFE-coated aluminum add TF to end of cat. . SPECIFICATIONS Horizontal System Cat. Overall Dimensions W x L x H cm Gel Bed Size W x L cm Buffer Volume ml Compatible Gel tray Gel Tray I.D. W x L cm Agarose Solution Required ml of Slots in Gel Tray PathlengthSlot Maximum of Samples HSU-2614 28 x 38 x10 26 x 14 2200 WGT-2614 24.7 x 14 173ml 5 2.5cm 510 HSU-2626 28 x 48 x10 26 x 26 2600 WGT-2626 24.7 x 26 320ml 8 2.5cm 816 HSU-2640 28 x 64 x10 26 x 40 3400 WGT-2640 24.7 x 40 494ml 8 4.25cm 816 Gel tray agarose required was calculated using a gel thickness of 0.5cm DESCRIPTION Three different sized High-Throughput Submarine Systems are designed for the rapid screening of samples from microtitre plates. Analyze high numbers of cloning products restriction fragments synthetic oligos or PCR products simultaneously. FEATURES Platinum electrode cassettes provide rapid sample velocity and clear focused bands without smiling. These cassettes are easily removed for for cleaning or replacement. High quality heavy duty construction 3 year warranty with vast array of accessory options. Three sizes of High-Throughput Systems are offered with the following gel bed sizes 26cmw x 14cml 26cmw x 26cml 26cmw x 40cml Gel Tray Specifications 1 All gel trays are winged for easy handling - no more sticking fingers into slippery buffer to extract gel tray. 2 Made from sturdy 6.4mm thick UV transparent acrylic. 3 Grooves in gel tray corners create agarose anchors to prevent gel flotation during electrophoresis. 4 Gel tray configuration allows single run or multiple simultaneous short runs. See Gel Tray Profiles. 3 options for gel casting tapered baffles external gel casters or continuously adjustable gel caster as shown on page 53. All combs are adjustable height and have an aluminum backing to keep the combs perfectly straight during gel casting with hot agarose. All combs come with microtitre format to allow multi- channel pipetting for accuracy and speed. Comb profiles are diagrammed at right to show the many configurations with or without markers. PTFE-coated combs allow faster well formation and are recommended when using low percentage or low melt agarose. Buffer recirculation ports allow maintenance of uniform pH and ionic strength and reduce frequency of buffer changes necessary during long runs. Tight-fitting safety cover reduces condensation during electrophoresis. Coolant can be recirculated beneath gel bed to provide constant temperature runs. Combs are printed with catalog and thickness for easy identification. Removable Electrode Cassette Design p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 53 HORIZONTALS Gel Tray Profiles Possible loading strategy for microtitre compatible combs are listed below. M marker lane and is shaded in blue 12 8 number of wells loading capability for microtitre compatible spacing SGC26-XX26 SGC26-XX25-1MA SGC26-XX51 SGC26-XX102 M-12-12-M M-8-8-8-M 8-8-8-M M-8-8-8-M-8-8-8-M M-12-12-M-12-12-M M-8-8-8-M-8-8-8-M-8-8-8-M-8-8-8-M-M M-12-12-M-12-12-M-12-12-M-12-12-M-M Loading options Continuously Adjustable Gel Caster Continuously Adjustable Caster cat. AGCT-27940 is adaptable to any gel tray between 27cmw and from 9-40cml. The gel tray friction fits between 2 rubber gaskets using two finger cams. See page 54. ORDERING INFORMATION CAT. DESCRIPTION HIGH-THROUGHPUT SUBMARINE SYSTEMS HSU-2614 High-Throughput Submarine System CE gel bed dimensions 26cmw x 14cml HSU-2626 High-Throughput Submarine System CE gel bed dimensions 26cmw x 26cml HSU-2640 High-Throughput Submarine System CE gel bed dimensions 26cmw x 40cml Related Products AGCT-27940 Continuously Adjustable Gel Caster fits gel trays 27cmw and from 9-40cml GCT-26-141 External Gel Caster fits one 26cmw x 14cml tray GCT-26-142 External Gel Caster fits two 26cmw x 14cml trays GCT-26-261 External Gel Caster fits one 26cmw x 26cml tray GCT-26-262 External Gel Caster fits two 26cmw x 26cml trays GCT-26-401 External Gel Caster fits one 26cmw x 40cml tray GCT-26-402 External Gel Caster fits two 26cmw x 40cml trays WGT-2614 Replacement Gel Tray UVT 26cmw x 14cml for HSU-2614 WGT-2626 Replacement Gel Tray UVT 26cmw x 26cml for HSU-2626 WGT-2640 Replacement Gel Tray UVT 26cmw x 40cml for HSU-2640 EPS-300 X Power Supply CE 1-300V 1-500mA EPS-600 High Current Power Supply CE 5-600V 1-500mA recommended for HSU-2640 Microtitre Comb Profiles WGT-2614 WGT-2626 14cm 26cm 40cm WGT-2640 2.5cm 2.5cm 4.25cm w w w. c b s s c i e n t i f i c . c o m54 HORIZONTALS Continuously Adjustable Gel Casters AGCT-16520 AGCT-21826 AGCT-27940 Complete System Adjustable caster with leveling base and cut-out for handling and storage 2 rubber gaskets Replacement gaskets set of 2 Single AGCT-16520 or dual AGCT-21826 or AGCT-27940 finger cams 1 2 3 4 5 With cam levers in horizontal position slide adjustable gate far enough away from stationary gasket to fit your gel trays. Place gel tray in position against stationary gasket as shown. Slide adjustable gate until snug against gel trays. Press cam levers into vertical position to lock gate in place. Pour gels. After gel has cooled return levers to horizontal position and slide away from gel trays. 5 easy casting steps DESCRIPTION The Continuously Adjustable Horizontal Gel Casters fit a wide range of gel trays and are easy to use. All C.B.S. Scientific gel trays are accommodated by at least one of the 3 sizes offered. These versatile casters can also be used to fit any other vendors gel trays within the limits of each size. The gel tray is sandwiched between 2 rubber gaskets using an adjustable gate that can be locked into place using a finger cam. Multiple gel trays can also be cast simultaneously side by side in this sys- tem. A convenient cutout on the base can be used to carry the caster or to hang it when not in use. To determine which of the 3 casters would best suit your needs refer to the list of compatible C.B.S. Scientific horizontal units and the range of gel tray dimensions accepted by each caster in the tables below. Horizontal Unit and Caster Compatibility x depicts that gel tray of unit will fit Caster Ordering Information and Gel Tray Dimensions C.B.S. Scientific Horizontal cat. Caster cat. AGCT-16520 Caster cat. AGCT-21826 Caster cat. AGCT-27940 MHU-102 MHU-202 MHU-402 MHU-602 HSU-014 HSE-014 WHSE-014 WMGE-600 x x x HSU-020 HSE-020 x x HSU-030 HSE-030 HSU-040 HSE-040 HSU-2614 HSU-2626 HSU-2640 x Continuously Adjustable Horizontal Gel Casters Versatile Casters adjust to many horizontal gel tray sizes Continuously Adjustable Caster Cat. Maximum Width of Gel Tray Minimum Length of Gel Tray Maximum Length of Gel Tray AGCT-16520 16cm 8cm 20cm AGCT-21826 21cm 8cm 26cm AGCT-27940 27cm 9cm 40cm ORDERING INFORMATION CAT. DESCRIPTION CONTINUOUSLY ADJUSTABLE GEL CASTERS AGCT-16520 Continuously Adjustable Gel Caster fits gel trays 16cmw and from 8-20cml AGCT-21826 Continuously Adjustable Gel Caster fits gel trays 21cmw and from 8-26cml AGCT-27940 Continuously Adjustable Gel Caster fits gel trays 27cmw and from 9-40cml p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 55 POWER SUPPLIES Power Supplies Choice of 3 Programmable Power Supplies that are light compact and stackable Parameter SPECIFICATIONS for EPS-300 X SPECIFICATIONS for High Current EPS-250 X SPECIFICATIONS for EPS-600 Applications SDS-PAGE Lipoprotein Gels Horizontal Electrophoresis DGGECDGETTGEDTSK SDS-PAGE Electroblotting Western tank Semi-Dry Northern SDS-PAGE Mega-Gel Horizontal Agarose Gels Vertical Agarose Gels Semi-Dry transfer Large Format Mini-Gel tank transfer Input 100 - 240 VAC 50-60 Hz 100 - 240 VAC 50-60 Hz 100 - 240 VAC 50-60 Hz Output voltage V 1-300 1 V steps CC or CV 5 250 1 V steps CC or CV 5 600 1 V steps CC or CV Output current mA 1 500 1 mA steps 10-2500 1 mA steps 1 500 1 mA steps Maximum output power 90 W 200 W 120 W Output jacks 4 pairs color coded recessed for safety 4 pairs color coded recessed for safety 4 pairs color coded recessed for safety Output connector 4mm plugs 4mm plugs 4mm plugs Timer Minutes 1-1440 minutes 24 hours 1 min. increment 1-1440 minutes 24 hours 1 min. increment 1-1440 minutes 24 hours 1 min. increment User interface 3 digit red LED membrane keypad 3 digit red LED membrane keypad 3 digit red LED membrane keypad Dimension L x W x H 320 x 240 x 73 mm 320 x 240 x 73 mm 320 x 240 x 73 mm Weight Net 2.4 kg 5.3 lb Gross 3.4 kg 7.5 lb Net 2.4 kg 5.3 lb Gross 3.4 kg 7.5 lb Net 2.4 kg 5.3 lb Gross 3.4 kg 7.5 lb Operating Conditions Indoor use 0-40C Relative humidity 0 to 90 non-condensing Altitude 2000m Indoor use 0-40C Relative humidity 0 to 90 non-condensing Altitude 2000m Indoor use 0-40C Relative humidity 0 to 90 non-condensing Altitude 2000m ORDERING INFORMATION CAT. DESCRIPTION POWER SUPPLIES EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA High Voltage Programmable Power Supply FEATURES The EPS-3000P-II power supply is designed to provide constant voltage current or power in both Manual and Programmed modes of operation commonly used in electrophoresis applications. Universal input power supply accepts all domestic and international voltages. The EPS-3000P-II can deliver up to 400W of total output power. One to four sets of electrophoresis cells can be connected in parallel and run simultaneously. Safety features include no load detection sudden load change protection over voltage protection and automatic power failure recovery Parameter SPECIFICATIONS for EPS-3000P-II Applications DNA Sequencing Large Format Vertical Electrophoresis Input 85-265 VAC 50-60 Hz Output voltage V 20-3000 1 V steps CC or CV Output current mA 1-400 1 mA steps Maximum output power 400W Temperature Control 0-90C adjustable in 1C steps Output jacks 4 pairs color coded recessed for safety Output connector 4mm plugs Timer 0-100 hours User interface 3 digit red LED membrane keypad Dimension L x W x H 31 x 27 x 13.3cm Weight Net 2.4 kg 5.3 lb Gross 3.4 kg 7.5 lb Operating Conditions Indoor use 0-40C Relative humidity 0 to 90 non-condensing Altitude 2000m ORDERING INFORMATION CAT. DESCRIPTION POWER SUPPLIES EPS-300 X Power Supply CE 1-300V 1-500mA EPX-250 X High Current Power Supply CE 2-250V 10-2500mA EPS-600 Power Supply CE 5-600V 1-500mA FEATURES All 3 types of power supplies have the same compact light and stackable design Universal input power supply accepts all domestic and international voltages. Parameters are set by pressing UPDOWN arrow buttons in combination with a MODE button Power output is controlled by setting a maximum value for voltage or current. The power supply automatically crosses over or switches the limiting parameter according to the pro- grammed limits as the resistance of the electrical load changes during a run. Safety features on all three models include no load detection sudden load change protec- tion over voltage protection and automatic power failure recovery w w w. c b s s c i e n t i f i c . c o m56 PCR WORKSTATIONS The Optimizer PCR Workstation is designed to protect against contamination in sen- sitive PCR amplification reactions in the following ways 1 UV irradiation of working area prior to use blocks replication of contaminating DNA sequences by causing adjacent pyrimidines to undergo dimerization. 2 This Dead Air Box protects against cross or airborne contamination by limiting exposure of experimental set-up to open lab environment. FEATURES Plenty of interior workspace for PCR reaction supplies and equipment. Safety Interlock factory installed interlock automatically shuts off UV light when hood is opened. Ergonomic design for comfortable posture Single or dual access panels close during UV irradiation then slide into storage compartment in base during experimental procedures. Hinged glass door allows easy access to interior workspace for cleaning and placement of large instruments or supplies. Dual fluorescent lamps assure excellent work space visibility. Dual 254nm ozone-free UV lamps with convenient switch for timed decontamination of workspace. Available either with acid-resistant black formica or durable stainless steel. A stainless steel ceiling angle reflector with stainless steel work surface option yields greater UV reflection resulting in a 10 increase in UV dose in PCR Workstations with a single UV bulb and a 20 increase with a dual UV bulb. DIMENSIONS The PCR Workstation is available in 3 sizes with the following exterior dimensions 24h x 24d x 30w 24h x 24d x 36w 24h x 24d x 48w Optimizer PCR Workstation P-030-202-SS stainless steel working surface panel clo- sure shown here in storage position with dual UV lights 24h x 24d x 30w. Made in the USA. PCR is a patented process of Hoffmann-LaRoche. Use of the PCR process requires a license. Optimizer PCR WORKSTATIONS Stainless Steel Worksurface Option P-030-02-SS P-030-202-SS P-036-02-SS P-036-202-SS P-048-02-SS P-048-202-SS Black Formica Worksurface Option P-030-02 P-030-202 P-036-02 P-036-202 P-048-02 P-048-202 Complete System Stainless steel or black formica work surface Safety Interlock automatically shuts off UV lights when Workstation is opened Tempered safety glass door Electrical outlet mounted in ceiling Country specific Two fluorescent lamps mounted in stainless steel ceiling Dual UV Germicidallamps ozone-free with 12 hour countdown timer with time-hold position Single or double panel closures Optimizer PCR Workstations Protects against PCR reaction contamination Transform your PCR Workstation into a mobile work area that doesnt infringe on valuable bench space with accessory table and locking casters as shown above. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 57 PCR WORKSTATIONS Customized Ocular Fitting for Microscope This customized fitting allows a microscope to be placed in the PCR Workstation while maintaining the integrity of UV protection and preserving the sterility of the workstation environment. A small oval cut-out is removed from the tempered glass fascia to accommodate the oculars which is then covered in silicone. The silicone permits transmittance of only negligible amounts of UV light and is cut to securely wrap the oculars. The silicone also protects the sterility of the microscope stage area by preventing contaminating DNA from entering through the ocular cut-out. Must be factory installed. Dual UV Bulb Option Dual UV Bulbs deliver twice the intensity of UV light than that of the single and will help irradiate shadowed areas that might otherwise be inaccessible. The Dual UVbulb format is recommended when the researcher desires to use the Workstation to decontaminate apparatus and reagents more rapidly. Mobile Workstation Option Accessory table and locking casters transform the PCRWorkstation into a moveable work surface that doesnt infringe on valuable bench-top space as shown on previous page. OPTIONAL FEATURES AVAILABLE ORDERING INFORMATION CAT. DESCRIPTION OPTIMIZER PCR WORKSTATIONS P-030-202-SS Optimizer PCR Workstation 30 wide dual UV light stainless steel worksurface safety interlock P-036-202-SS Optimizer PCR Workstation 36 wide dual UV light stainless steel worksurface safety interlock P-048-202-SS Optimizer PCR Workstation 48 wide dual UV light stainless steel worksurface safety interlock P-030-202 Optimizer PCR Workstation 30 wide dual UV light black formica worksurface safety interlock P-036-202 Optimizer PCR Workstation 36 wide dual UV light black formica worksurface safety interlock P-048-202 Optimizer PCR Workstation 48 wide dual UV light black formica worksurface safety interlock Related Products T-030-28 Table for use with 30w PCR Workstation 28 high T-030-36 Table for use with 30w PCR Workstation 36 high T-036-28 Table for use with 36w PCR Workstation 28 high T-036-36 Table for use with 36w PCR Workstation 36 high T-048-28 Table for use with 48w PCR Workstation 28 high T-048-36 Table for use with 48w PCR Workstation 36 high TC-400 Table casters set of four locking swivel casters CWB-F20T12CW Replacement Fluorescent Cool White Bulb for P-030 or P-036 CWB-F30T12CW Replacement Fluorescent Cool White Bulb for P-048 GB-G15T8 Replacement Germicidal Bulb for P-030 or P-036 GB-G30T8 Replacement Germicidal Bulb for P-048 P-Ocular Customized Ocular Fitting factory Installed International customers need to specify which Country the PCR Workstation will be used in so that correct mains plug can be included. Factory installed at time of order w w w. c b s s c i e n t i f i c . c o m PRECAST GELS 58 SDS PAGE with 2 year shelf life and usurpassed resolution FEATURES Very strong gel makes handling easy. 2 year shelf life - never throw away another gel Unique acrylamide polymer matrix for exceptional protein separations Sharp clean consistently straight bands There are no combs or tape to remove shortening prep time. Reusable lower buffer saves time and money. You can save up to 75 of the buffer cost. All gels have the ability to expand the resolving range of a given gel by running the small proteins off the unsealed bottom of the gel. Long well fingers are fixed and extend above top of cassette for easy loading and to prevent sample cross-contamination. Red dye is used in the loading area for better well visualization in the 8cm x 10cm and 10cm x 10cm sizes. Simple TEO-Tricine buffer system. NOTE TEO-Tricine buffers MUST be used with these gels. Quick run times - run in as little as 30 minutes. Choice of 3 Cassette Sizes 10cmw x 8cml 10cmw x 10cml 15cmw x 10cml COMPATIBLE WITH C.B.S. Scientific Electrophoresis Systems Quadra Systems Cat. No. QNX-700 QNC-700 4-place Combo Cells fits 10 x 10cm gels Dual Cool Cat. No. DCX-700 2-place Combo Cell with freezer blocks fits 10 x 10cm gels Mini-Blotter Cat. No. EBU-204 with cooling base 4-place fits 8cm x 10cm and 10 x 10cm gels Midi-Blotter Cat. No. EBU-402 cooling base 2-place fits 10 x 15cm gels Dual Vertical Midi-Gel Cat. No. DCX-800 fits 15 x 10cm gels ClearPAGE is a trademark of C.B.S. Scientific. PAGEr and Lonza are trademarks of Lonza Corporation. Surelock and XCell are registered trademarks of Life Technology Corporation. Mini-PROTEAN and Tetra are registered trademarks of Bio-Rad Laboratories Corporation. Dual Mini-Verticals with internal cooling Cat. No. MGV-202 fits 10 x 10cm gels Cat. No. MGV-402 fits 10 x 8cm gels 16 ClearPAGE SDS precast gel 10cmw x 10cml 12 wells. Chicken Lysate- Lanes 45 7810 11 BSA- Lane 2 E.Coli- Lanes 39 BioRad Markers - Lanes 1612 COMPATIBILITY Cassette Size W x L cm Gel Box Manufacturer Compatible Gel Boxes 10 x 8 Bio-Rad Bio-Rad C.B.S. Scientific C.B.S. Scientific Mini-Protean II Mini-Protean Tetra Mini-Vertical MGV-302 Dual Mini-Vertical MGV-402 10 x 10 C.B.S. Scientific C.B.S. Scientific Invitrogen Lonza Dual Cool DCX-700 Quadra Systems QNX-700 QNC-700 X-cell PAGEr Minigel Chamber 15 x 10 C.B.S. Scientific Invitrogen DCX-800 Xcell4 requires replacement gasket BRGK-100 described on next page SPECIFICATIONS Cassette Size W x L cm Gel Dimensions W x L cm Gel Thickness Shelf Life Required Buffer 10 x 8 8.5 x 6.5 1.0mm 2 years TEO-Tricine 10 x 10 8.0 x 8.5 1.0mm 2 years TEO-Tricine 15 x 10 13.2 x 8.0 1.0mm 2 years TEO-Tricine ClearPAGE Precast Gels p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 PRECAST GELS 59 ClearPAGE SDS gels packages of 10 - Cat. s Acrylamide Gel Size cm W x L 1 well Cat. 2D well trough 1 marker lane Cat. 12 well Cat. 12 well 2 marker lanes Cat. 17 well Cat. 20 well Cat. 26 well Cat. 8 10 x 8 - - BK00812-10 - BK00817-10 - - 10 x10 - - FK00812-10 - FK00817-10 - - 10 10 x 8 - - BK01012-10 - BK01017-10 - - 10 x10 FK01001-10 - FK01012-10 - FK01017-10 - - 12 10 x 8 - - BK01212-10 - BK01217-10 - - 10 x10 FK01201-10 - FK01212-10 - FK01217-10 - - 15 x 10 - - - GK-01212-10 - GK-01220-10 GK-01226-10 16 10 x 8 - - BK01612-10 - BK01617-10 - - 10 x10 - - FK01612-10 - FK01617-10 - - 4 - 8 10 x 8 - - BK40812-10 - BK40817-10 - - 10 x10 - - FK40812-10 - FK40817-10 - - 4 - 12 10 x 8 - - BK41212-10 - BK41217-10 - - 10 x10 - - FK41212-10 - FK41217-10 - - 15 x 10 - - - GK-41212-10 - GK-41220-10 GK-41226-10 4 - 20 10 x 8 - - BK42012-10 - BK42017-10 - - 10 x10 FK42001-10 FK42002-10 FK42012-10 - FK42017-10 - - 15 x 10 - - - GK-42012-10 - GK-42020-10 GK-42026-10 Well Type Cassette Size W x L cm Recommended Load Volume 12-well 10 x 8 10 x 10 35 l 12-well 2 marker lanes 15 x 10 51 l 17l marker 17-well 10 x 8 10 x 10 17 l 20-well 15 x 10 30 l 26-well 15 x 10 20 l 2-D well trough 1 marker lane 10 x 10 300 l 17l marker Prep-Well 1 well 10 x 10 400 l Comb Load Volumes Gel Selection Chart Ordering information Minimum order of 30 gels Polyacrylamide Concentraion Size Separation Range 8 110-200 kDa 10 15-200 kDa 12 10-200 kDa 16 5-60 kDa 4-8 50-600 kDa 4-12 10-225 kDa 4-20 5-200 kDa Separation Range Long numbered well fingers extend above top of cassette to prevent sample carry-over. Red dye is used in the loading area in 10cm wide gels for better well visualization. This dye runs ahead of the brom- phenol blue marker. Snap-fit cassette opens easily Finger-locking cassettes Cassette Features Gel type expiration date and lot number 1 2 3 Gasket Replacement on Bio-Rad Units To use ClearPAGE Precast gels with Bio-Rad Mini-Protean Gel Boxes the green gasket is easily replaced with a white gasket cat. BRGK-100 provided by C.B.S. Scientific. 1 Pull green clamps away from sides of reservoir to access green gasket. 2 Pull green gasket out of groove. 3 Place white gasket into groove so that tapered side faces out. Your Mini-ProteanII or Mini-Protean Tetra is now ready to accept ClearPAGE 8x10cm precast gels. w w w. c b s s c i e n t i f i c . c o m PRECAST GELS 60 Easy to Use Remove SDS fix and stain in ONE STEP. No destain is needed. Fast Results in 10 to 30 minutes Sensitive 5-25ng of protein can be detected with low background Non-Hazardous Contains no methanol suited for regular use and disposal DESCRIPTION Instant Blue stain is a ready-to-use proprietary Coomassie G-250 stain that is optimal for obtaining well defined protein bands in a single step. Protein gel staining takes only 10-30 minutes because there is no need to wash fix microwave or destain. Instant Blue stain contains a propri- etary SDS-sequestering agent patent pending that makes it so fast and easy. Since SDS binds stain it normally must be removed to get good sensitivity. Usually some remains in the gel which contributes to background and requires destaining. The SDS-sequestering agent removes the SDS from the gel so no washes or destaining is necessary. Its special formulation has the benefits of high sensitivity ap- proximately 5-25ng of protein detected per band and low background interference-improving the overall gel resolu- tion. Additionally it contains no methanol and is ideally suited for regular use and disposal. Instant Blue A fast one-step stain Comparison of Marker on ClearPAGE 12 12 well SDS gel with or with Instant Blue stain. Molecular weights are the apparent weights in the ClearPAGE SDS gel system. 195 142 96 71 48 33 28 22 12 7.6 Instant Blue Stained Natural kDa Two-Color SDS Marker Stable and Sharp Recombinant Proteins Economical Use in place of unstained markers DESCRIPTION The recombinant proteins range in size form 7.6 kDa to 195 kDa. The orange markers are 28 kDa and 71 kDa the other 8 bands are blue . The ClearPAGE Two-Color SDS Marker is sold ready to use in a 1x LDS sample buffer with 600 l per vial catalog HM05160. The purity and band sharpness mean the markers can be used for visualizing the separation while running the gel checking effectiveness on your blot or as a routine marker in place of other unstained markers. An orangeblue prestained marker for SDS-PAGE Unsurpassed resolution and 2 year shelf life FEATURES Unique acrylamide polymer matrix for exceptional separations Sharp clean consistently straight bands There are no combs or tape to remove shortening prep time. Super-strong gel makes handling easy. Reusable lower buffer saves time and money. You can save up to 75 of the buffer cost. TEO gels have a neutral pH for a 2 year shelf life - never throw away another gel Long well fingers extend above top of cassette and to prevent sample cross-contamination. Green dye is used in the loading area for better well visualization. Simple TEO-Tricine buffer system. NOTE TEO-Tricine buffers MUST be used with these gels. SPECIFICATIONS Gel type DNANative gels Shelf life 2 years Overall Cassette Dimensions W x L x thickness 10 x 8 x 5mm 10 x 10 x 5mm Gel Dimensions W x L x thickness 8.5cm x 6.5cm x 1mm 8.0cm x 8.5cm x 1mm Number of Gels Box 10 Color of Gel Loading Area green Gel Thickness 1mm ClearPAGE Precast DNANative Gels packages of 10 - Cat. s acrylamide gel size cm W x L 12 well 17 well 10 10 x 8 BN01012-10 BN01027-10 10 x 10 GN01012-10 GN01027-10 2-8 10 x 10 GN20812-10 GN20827-10 3-20 10 x 8 BN32012-10 BN32027-10 10 x10 GN32012-10 GN32027-10 Gel Selection Chart Ordering information Superior DNA Separations Precast DNA and Native PAGE gels also have a long shelf life. These gels are a neutral pH and utilize a stacking system. Larger sample volumes such as 35 l12 well and 17 l17 well will still produce sharp bands. ClearPAGE Precast Gels DNANative ClearPAGE Precast Gels Polyacrylamide Concentration Optimal Separation Range Range 10 80-500 BP 60-3000 BP 2-8 300-800 BP 300-3000 BP 3-20 20-300 BP 20-3000 BP Separation Range Long numbered well fingers extend above top of cassette to prevent sample carry-over. Green dye is used in the load- ing area in 10cm wide gels for better well visualization. This dye runs ahead of the brom- phenol blue marker. Snap-fit cassette opens easily Finger-locking cassettes Cassette Features Gel type expiration date and lot number p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 PRECAST GELS 61 ORDERING INFORMATION CAT. DESCRIPTION ClearPAGE BUFFERS STAINS MARKERS FB30001 Antioxidant 1ml FB300010 Antioxidant 10ml FB31010 LDS Sample Buffer 4X 10 ml FB32001 DTT reducer 10X 1 ml FB50053 TEO-Tricine SDS running buffer 20X 50 ml for reducing or non-reducing conditions FB50500 TEO-Tricine SDS running buffer 20X 0.5L for reducing or non-reducing conditions FB14053 Turbo buffer SDS running buffer 20X 50ml FB14500 Turbo buffer SDS running buffer 20X 0.5L FB82500 Transfer buffer with SDS 10X 0.5L FB82600 Transfer buffer without SDS 10X 0.5L HG73010 Instant Blue stain 1 L HG73050 Instant Blue stain trial size 50ml HM05160 2-color marker 0.6ml HM05103 2-color marker trial size 30l ClearPAGE DNANATIVE BUFFERS GB33002 DNANative sample buffer 4 x 2ml GB33010 DNANative sample buffer 4 x 10ml GB61050 DNANative running buffer 20 x 50ml GB61500 DNANative running buffer 20 x 0.5L FB82600 Transfer buffer without SDS 10X 0.5L ClearPAGE BLOTTING AND DRYING FB82500 TGS transfer buffer 10x 500ml HP19001 Blot sandwich NC 90 x 85mm 0.2l nitrocellulose1mm blotting paper 2 pack HP19020 Blot sandwich NC 90 x 85mm 0.2l nitrocellulose1mm blotting paper 20 pack HP29301 Blot sandwich PVDF 90 x 85mm 0.2l PVDF1mm blotting paper 2 pack HP29320 Blot sandwich PVDF 90 x 85mm 0.2l PVDF1mm blotting paper 20 pack HB04510 Gel drying solution 1L HB02520 Precut cellophane 25cm x 25cm 20 sheets HB90001 Drying frame with 8 clamps with cellophane 25cm x 25cm 20 pieces of cellophane ClearPAGE SAMPLER KITS FM90002 ClearPAGE Sampler Kit Includes 2 each 10cm x 10cm SDS gels of customers choice trial buffers and instructions. FM90003 ClearPAGE Sampler Kit Includes 2 each 10cm x 10cm DNANative gels of customers choice trial buffers and instructions. FM90005 ClearPAGE Super Sampler Kit Includes 2 each 10cm x 10cm SDS gels of customers choice trial buffers trial size 2-color marker trial size Instant Blue stain and instructions. FM90006 ClearPAGE Super Sampler Kit Includes 2 each 10cm x 8cm SDS gels of customers choice trial buffers trial size 2-color marker trial size Instant Blue stain and instructions. FM90007 ClearPAGE Super Sampler Kit Includes 2 each 15cm x 10cm SDS gels of customers choice trial buffers trial size 2-color marker trial size Instant Blue stain and instructions. ClearPAGE Precast Gels Buffers ClearPAGE TEO-Tricine SDS Running Buffer FB50053 FB50500 is opti- mized for use with ClearPAGE Precast Gels to provide the best running con- ditions and optimal sample resolution. Based on a TEO-Tricine buffer system this gives improved resolution at lower molecular weights while retaining excellent resolution at the high molecular weights. This buffer can be used under reducing or non-reducing conditions. Separation pattern is similar to that produced by a conventional MOPS buffer system. ClearPAGE LDS Sample Buffer FB31010 has been specifically formulated for use with ClearPAGE gels. The ions in the sample buffer match the gel buffer and the buffer has a higher density making it compatible with the density of the running buffer. The buffer contains two tracking dyes. The Red dye is designed to monitor the pH of the buffer. A color change from red to yellow indicates a shift in pH indicating that the buffer was poorly made or recycled too many times. ClearPAGE Turbo Running Buffer FB14053 FB14500 Tris-MOPS buffer system which provides a separation profile similar to MES buffer used with BIS-TRIS gels. This buffer can be for reduced by adding antioxident if needed. This buffer is best when used with acrylamide gel percentages of 12 or less. ClearPAGE DNANative Running Buffer GB61050 GB61500 is opti- mized for use with ClearPAGE DNANative Gels to provide the best running contitions and optimal sample resolution. Based on a TEO-Tricine buffer sys- tem this gives improved resolution at lower molecular weights while retain- ing excellent resolution at the high molecular weights. Separation pattern is similar to that produced by a conventional MOPS buffer system. All ClearPAGE buffers reagents and accessories have been specifically designed and formulated for use with the ClearPAGE gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manu- facturers buffers. Use only ClearPAGE formulated buffers with ClearPAGE gels. w w w. c b s s c i e n t i f i c . c o m62 MUTATION DETECTION 3 Different Instruments for Mutation Screening APPLICATIONS DETECTING SINGLE BASE DNA CHANGESMUTATIONS Detecting Polymorphisms in double stranded DNA Determining DNA fragment melting points Detects Single Strand Conformational Polymorphisms Over the past 30 years several gel-based techniques have been developed to rapidly and economically reveal single-base mutations in DNA fragments. C.B.S. Scientific has developed 3 different instruments that can be used to scan mutations by DGGE CDGE TTGE SSCP and other techniques. Choose the instrument that fits your application from the chart below. APPLICATION CHART Cat. DGGE TTGE SSCP CDGE DGGEK-1001 DGGEK-2001 DGGEK-2401 TTGEK-2001 TTGEK-2401 DTSK-2001 DTSK-2401 Both DGGE and TTGE rely on establishing a gradient of either solvent UreaFormamide or temperature in which the target fragments will undergo conformational transition melt. This sequence dependent information will determine the theoreti- cal melting behavior of the target fragment after PCR amplification. If the sequence is known then primerprobe design can be made using certain software design programs 1. If not the melting range can be revealed by running perpendicular DGGETTGE gels. The third mutation detection technique SSCP differentiates between normal and mutant duplex DNA by denaturing the DNA to form single stranded molecules of equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing form different three-dimensional structures. These structures differ in electrophoretic mobility and can be separated on a non-denaturing polyacrylamide gel. DGGE AND CDGE Denaturing Gradient Gel Electrophoresis DGGE is a powerful genetic analysis technique that can be used for detecting sin- gle base changes and polymorphisms in genomic 23 cloned and PCR amplified DNA 34. Two of the most valuable uses for DGGE in human and microbial genetics are in directly detecting single base changes that cause disease and in detect- ing polymorphisms with DNA probes for genetic-linkage analysis. In DGGE conformational transitions of multiple nucleic acid complexes are induced by an increasing concentration of solvent UreaFormamide at a constant temperature. Clinical applications of DGGE include a rapid and effective method for screening samples for genetic mutations and variants. Also DNA fragment melting points can be determined using perpendicular DGGE 2. In contrast to DGGE CDGE Constant De- naturant Gel Electrophoresis uses a single solvent percentage to induce partial melting of DNA fragments as they enter the gel. The disadvantage of CDGE is that only a single melting domain can be interrogated. TTGE Destabilization of nucleic acid complexes can be studied using acrylamide gels that contain a uniform solvent concentration as in CDGE but with an increasing temperature gradient 6. Since the temperature of the entire gel is uniformly raised over a period of time this technique has been termed TTGE or Temporal Temperature Gradient Electrophoresis 7. This tech- nique incorporates many improvements over DGGECDGE especially when studying multiple melting domains 8. SSCP Single Strand Conformational Polymorphism SSCP reveals differences in electrophoretic mobility between normal and mu- tant single strands of DNA 9. In SSCP normal and mutant duplex DNA are denatured to form single stranded molecules of Mutation Detection Discussion p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 63 MUTATION DETECTION equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing form different three-dimensional structures. These structures differ in electrophoretic mobility and can be separated on a chilled non-denaturing polyacrylamide gel. The electrophoretic mobility of these conformers change depending on temperature and buffer ionic strength. For accurate characterization of mutations within these re-folded single strands it is essential that the buffer temperature be tightly controlled within each electrophoresis procedure usually between 4-15C. If the gel temperature is not precisely controlled the resolution will suffer because of the loss of intrastrand base pairing and change in the overall shape of the 3-dimensional conformer. The Cipher Genetic Analysis Systems Cat. s DTSK-2001 DTSK-2401 are equipped with a large diameter cooling coil in the anode buffer chamber. The cooling coil is connected to an external refrigerated water bath chiller. The Cipher Instru- ment automatically mixes the buffer and regulates the overall temperature it is capable of holding the buffer temperature within 0.2C of the set temperature assuring consistent intra- and inter-gel conditions. The Cipher Genetic Analysis System is the only system offered that can perform all four applications DGGE CDGE TTGE and SSCP. All Mutation Detection Systems feature EPS-300 X Power Supply 100-240 VAC 50-60 Hz 300V 500mA 90W Gel cassettes Tank with safety interlock lid HeaterStirrerBuffer Cycler Mini-peristaltic pump Gradient maker Combs Spacers Glass plates Gel Wrap gaskets White spring clamps Buffer siphon pump REFERENCES 1 Tian-Jian Chen Richard G. Boles Lee-Jun Wong Detection of Mito- chondrial DNA Mutations by Temporal Temperature Gradient Gel Electrophoresis. 1999. Clinical Chemistry 458 1162- 1167. 2 S.G. Fischer and L.S. Lerman 1983 PNAS 801579. 3 Guldberg P. Henriksen K.F. and Guttler F. 1993. Molecular analysis of phenylketonia in Denmark 99 of the mutations detected by denaturing gradient gel electrophoresis. Genomics 17141-146. 4 Moyret C. Theillet C. Puig P.L. Moles J-P. Thomas G. and Hamelin R. 1994. Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene. Oncogene 91739-1743. 5 Sheffield V.C. Beck J.S. Kwitek A.E. Sandstrom D.W. and Stone E.M. 1993. The sensitivity of single-strand conformation polymor- phism analysis for the detection of single base substitutions. Genom- ics 16 325-332. 6 K. Yoshino K. Nichigaki Y Husimi. 1991 Temperature sweep gel electrophoresis a simple method to detect point mutations. Nucleic Acids Res. 1931-53. 7 J. Bjorheim S. Lystad A. Lindblom U. Kressner et al. 1998 Mutation analysis of KRAS exon 1 comparing three different techniques tempo- ral temperature gradient electrophoresis constant denaturant capillary electrophoresis and allele specific polymerase chain reaction. 1998 Mutat Res 403 103-112. 8 A.H Fanrleitner N. Kreuzinger G.G Kavka et al 2000 Compara- tive analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis in separating Escherichia Coli uidA amplicons differing in single base substitutions. Letters in Applied Microbiology 30 427-431. 9 Orita M. Sekiya T. and Hayashi K. 1990. DNA sequence polymor- phisms in Alu repeats. Genomics 8 271-278. 10 Orita M. Sekiya T. and Hayashi K. 1989a. Rapid and sensitive detection of point mutations and DNA polymorphisms using the poly- merase chain reaction. Genomics 5 874-879. 11 Orita M. Iwahana H. Kanazawa H. Hayashi K and Sekiya T. 1989b. Detection of polymorphisms of human DNA by gel electro- phoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. 86 2766-2770. Ethidium Bromide stained DGGE gel of -Globin gene from S.E. Asian population after PCR of fragment II. N Normal allele E mutation on codon 26 17 mutation on codon 17 Patient Control IVS-1 intervening sequence. 1. Samples run on a 45-75 gradient for 5 hours at 60C at 200V constant current in TAE. N17 N NE NE NE NE N NE NE N NE NE NE EE N NE N N N N N Control17 Control IVS-1 EE NE N NE N N17 E17 N E 17 N RESULTS w w w. c b s s c i e n t i f i c . c o m64 MUTATION DETECTION APPLICATIONS Detecting single base DNA changesmutations Detecting polymorphisms in double stranded DNA Determining DNA fragment melting points Cipher DGGE Electrophoresis Systems Extremely accurate and highly reliable technique for mutation detection FEATURES C.B.S. Scientific offers 4 different sized Cipher DGGE Systems which are reliable and easy-to-use. Superior Temperature stability Heating element and thermostat maintain excellent temperature stability 0.2 and minimize thermal stratification during DGGE. Buffer Cycler eliminates need of an external peristaltic pump to recycle buffer Can run up to 8 gels at a time. This system lends itself well to the use of fluorescence based assays whose images can be captured on a fluorimeter directly through optional Borosilicate glass plate sets. A mini-peristaltic pump MPP-100 used in conjunction with the gradient maker produces accurate and consistent gradient gels Single or dual gel cassettes are offered Buffer tank dimensions have been reduced to use bench space more efficiently Safety cover has electrical interlock for protection and to help maintain temperature and reduce evaporation To avoid any incompatibility issues the EPS-300 X CE Power Supply is included because its specifications are suited to run all DGGE applications. Simplified casting method using GelWrap gasket for both perpendicular and vertical gels. Cipher DGGE System 2-place Cat. DGGEK-1001 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply 1 dual gel cassetteupper reservoir with platinum electrodes 1 buffer displacement tank Glass safety cover with electrical interlock HeaterStirrerBuffer Cycler 4 sets of spacers 2 sets for vertical DGGE and 2 sets with injection ports for perpendicular DGGE gel casting 4 combs 2 each 1-well combs and 2 each 16-rectangular well combs Buffer siphon pump 2 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 4 Gel Wrap gaskets White spring clamps Cipher DGGE System 4-place Cat. DGGEK-2401 1 Reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply 2 dual gel cassettesupper reservoirs with platinum electrodes Safety cover with electrical interlock HeaterStirrerBuffer Cycler 8 sets of spacers 4 sets for vertical DGGE and 4 sets with injection ports for perpendicular DGGE gel casting 8 combs 4 each 1-well combs and 4 each 16-rectangular well combs Buffer siphon pump 4 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 8 Gel Wrap gaskets White spring clamps Cipher DGGE System 2-place Cat. DGGEK-2001 Same components as DGGEK-1001 EXCEPT with 2 SINGLE gel cassettes instead of 1 dual cassette and no buffer displacement tank. Cipher DGGE System 8-place Cat. DGGEK-4801 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply 4 dual gel cassettesupper reservoirs with platinum electrodes Safety cover with electrical interlock HeaterStirrerBuffer Cycler 16 sets of spacers 8 sets for vertical DGGE and 8 sets with injection ports for perpendicular DGGE gel casting 16 combs 8 each 1-well combs and 8 each 16-rectangular well combs Buffer siphon pump 8 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-100 Gradient maker 50mls per side 16 Gel Wrap gaskets White spring clampsSPECIFICATIONS Model Cat. DGGEK-1001 DGGEK-2001 DGGEK-2401 DGGEK-4801 of cassettes 1 dual cassette 2 single cassettes 2 dual cassettes 4 dual cassettes of gels 2 gels 2 gels 4 gels 8 gels gel dimensions W x L cm 15 x 20 15 x 20 15 x 20 15 x 20 footprint W x L x H cm 25.4 x 54.6 x 34.3 25.4 x 54.6 x 34.3 25.4 x 54.6 x 34.3 25.4 x 74.9 x 34.3 REFERENCES 1 S.G. Fischer and L.S. Lerman 1983 PNAS 801579. 2 Guldberg P. Henriksen K.F. and Guttler F. 1993. Molecular analysis of phenylketonia in Denmark 99 of the mutations detected by denaturing gradient gel electrophoresis. Genomics 17141-146. 3 Moyret C. Theillet C. Puig P.L. Moles J-P. Thomas G. and Hamelin R. 1994. Relative ef- ficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene. Oncogene 91739-1743. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 65 MUTATION DETECTION Vertical Comb Options deeper wells overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716 19.1 16 0.75 5.2 55 VGC-0720 17.7 20 0.75 4.2 42 VGC-0722 17.7 22 0.75 4.35 25 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 55 VGC-1022 17.7 22 1.0 4.35 57 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1522 17.7 22 1.5 4.35 85 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 Vertical Comb Options short wells overall length 13.1cm Cat. Tooth depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716-177 10 16 0.75 5.2 29 VGC-0720-177 10 20 0.75 4.2 23 VGC-0722-177 10 22 0.75 4.35 25 VGC-0729-177 10 29 0.75 2.9 16 VGC-0730-177 10 30 0.75 2.8 16 VGC-1016-177 10 16 1.0 5.2 39 VGC-1020-177 10 20 1.0 4.2 32 VGC-1022-177 10 22 1.0 4.35 32 VGC-1029-177 10 29 1.0 2.9 22 VGC-1030-177 10 30 1.0 2.8 21 VGC-1516-177 10 16 1.5 5.2 58 VGC-1520-177 10 20 1.5 4.2 47 VGC-1522-177 10 22 1.5 4.35 49 VGC-1529-177 10 29 1.5 2.9 33 VGC-1530-177 10 30 1.5 2.8 32 Gel Casting Options Option 1 Multi-Caster Kit Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough 4 sets notched glass plate sets 4 sets of spacers 4 combs polycarbonate separation sheets. See page 23 for assembly diagram or online video instructions http www.cbsscientific.cominstructionvideos.aspx. ORDERING INFORMATION CAT. DESCRIPTION CIPHER DGGE ELECTROPHORESIS SYSTEMS DGGEK-1001 Cipher DGGE Electrophoresis System 2-place 1 dual cassette Specify 110V or 220V DGGEK-2001 Cipher DGGE Electrophoresis System 2-place 2 single cassettes Specify 110V or 220V DGGEK-2401 Cipher DGGE Electrophoresis System 4-place 2 dual cassettes Specify 110V or 220V DGGEK-4801 Cipher DGGE Electrophoresis System 8-place 4 dual cassettes Specify 110V or 220V Related Products VGS0720RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 0.75mm thickness x 22cml VGS1020RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.0mm thickness x 22cml VGS1520RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.5mm thickness x 22cml VGC-0701-177 Comb for Perpendicular DGGE 0.75mm thick x 1 well VGC-1001-177 Comb for Perpendicular DGGE 1.0mm thick x 1 well VGC-1501-177 Comb for Perpendicular DGGE 1.5mm thick x 1 well VGS-0720R-177 Gel Wrap Spacer Set 0.75mm thick x 22cm with rounded bottom corners VGS-1020R-177 Gel Wrap Spacer Set 1.0mm thick x 22cm with rounded bottom corners VGS-1520R-177 Gel Wrap Spacer Set 1.5mm thick x 22cm with rounded bottom corners VGE-0720-177 Gel Wrap Gasket to cast one DGGE gel 0.75mm thick VGE-1020-177 Gel Wrap Gasket to cast one DGGE gel 1.0mm thick VGE-1520-177 Gel Wrap Gasket to cast one DGGE gel 1.5mm thick NGP-17720NR Gel Wrap Glass plate set back plate has rounded bottom corners 17.7cm w x 22 cm h NGP-17720DNR Gel Wrap Glass plate set Borosilicate back plate with rounded bottom corners 17.7cm w x 22 cm h NGP-17720-N-LPF Gel Wrap Glass Plate Set both plates are Borosilicate. Back plate has rounded bottom corners 17.7cm w x 22 cm h GCC-404-177K Multiple Gel Casting Chamber Kit 4-place casts up to 4 each 15cm x 20cm gels VGS-0720C-177 Multiple Gel Casting Spacer Set 0.75mm thick x 22ml VGS-1020C-177 Multiple Gel Casting Spacer Set 1.0mm thick x 22ml VGS-1520C-177 Multiple Gel Casting Spacer Set 1.5mm thick x 22ml NGP-17720-N Multiple Gel Casting Chamber Glass Plate Set 7.7cmw x 22cmh NGP-17720-DN Multiple Gel Casting Chamber Glass Plate Set with Borosilicate back plate 7.7cmw x 22cmh NGP-17720-N-LPF Multiple Gel Casting Chamber Glass Plate Set both plates are Borosilicate 7.7cmw x 22cmh Option 2 Gel Wrap Casting System C.B.S. Scientific has developed a leak-proof gasket Gel Wrap that is a simplified method for casting perpendicular and vertical gels. Gel Wrap seals the glass plate sandwich for gel casting quickly and easily. Each DGGEK System comes with Gel Wrap gaskets and all components necessary to cast gels by this method. See page 23 for more detailed description or online video instructions httpwww.cbsscientific.cominstructionvideos.aspx. Maximum loading volume is calculated by taking 75 of the total tooth volume. w w w. c b s s c i e n t i f i c . c o m66 MUTATION DETECTION Cipher TTGE System 4-place Cat. TTGEK-2401 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply Programmable HeaterStirrerBuffer Cycler with temperature ramping capability 2 acrylic dual gel cassettesupper reservoir with platinum electrodes Safety cover with electrical interlock Buffer siphon pump 8 combs 4 each 1 well and 4 each 16 well combs 8 sets of spacers 4 with injection ports for perpendicular gel casting 4 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 8 Gel Wrap gaskets White spring clamps Cipher TTGE System 2-place Cat. TTGEK-2001 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply Programmable HeaterStirrerBuffer Cycler with temperature ramping capability 2 acrylic gel cassettesupper reservoir with platinum electrodes Safety cover with electrical interlock Buffer siphon pump 4 combs 2 each 1 well and 2 each 16 well combs 4 sets of spacers 2 with injection ports for perpendicular gel casting 2 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 4 Gel Wrap gaskets White spring clamps APPLICATIONS Detecting single base DNA changesmutations Detecting polymorphisms in double stranded DNA Determining DNA fragment melting points Extremely accurate and highly reliable Mutation Detection Systems for DGGE TTGE and CDGE FEATURES C.B.S Scientific offers 2 different sized Cipher TTGE Systems which are reliable and easy-to-use. Superior Temperature stability Heating element and thermostat maintain excellent temperature stability 0.2 and minimize thermal stratification during DGGE. Programmable heaterstirrer can be programmed for TTGE or DGGE applications. Buffer Cycler eliminates need of an external peristaltic pump to recycle buffer This system lends itself well to the use of fluorescence based assays whose images can be captured on a fluorimeter directly through optional Borosilicate glass plate sets. A mini-peristaltic pump MPP-100 used in conjunction with the GM-40 gradient maker produces accurate and consistent gradient gels Single or dual gel cassettes are offered which have been designed to eliminate the need of agarose plugs. Safety cover has electrical interlock for protection and to help maintain temperature and reduce evaporation To avoid any incompatibility issues the EPS-300 X CE Power Supply is included because its specifications are suited to run all DGGE and TTGE applications. Simplified casting method using Gel Wrap gasket for both perpendicular and vertical gels DGGE AND CDGE Denaturing Gradient Gel Electrophoresis DGGE is a powerful genetic analysis technique that can be used for detecting single base changes and polymorphisms in genomic 12 cloned and PCR amplified DNA23. Two of the most valuable uses for DGGE in human and microbial genetics are in directly detecting single base changes that cause disease and in detecting polymorphisms with DNA probes for genetic-linkage analysis. In DGGE conformational transitions of multiple nucleic acid complexes are induced by an increasing concentration of solvent UreaFormamide at a constant temperature. Clinical applications of DGGE include a rapid and effective method for screening samples for genetic mutations and variants. Also DNA fragment melting points can be determined using perpendicular DGGE1. In contrast to DGGE CDGE Constant Denaturant Gel Electrophoresis uses a single solvent percentage to induce partial melting of DNA fragments as they enter the gel. The disadvantage of CDGE is that only a single melting domain can be interrogated. TTGE Destabilization of nucleic acid complexes can be studied using acrylamide gels that contain a uniform solvent concentration as in CDGE but with an increasing temperature gradient4. Since the temperature of the entire gel is uniformly raised over a period of time this technique has been termed TTGE or Temporal Temperature Gradient Electrophoresis5. This tech- nique incorporates many improvements over DGGECDGE especially when studying multiple melting domains6. REFERENCES 1 S.G. Fischer and L.S. Lerman 1983 PNAS 801579. 2 Guldberg P. Henriksen K.F. and Guttler F. 1993. Molecular analysis of phenylketonia in Denmark 99 of the mutations detected by denaturing gradient gel electrophoresis. Genomics 17141-146. 3 Moyret C. Theillet C. Puig P.L. Moles J-P. Thomas G. and Hamelin R. 1994. Relative ef- ficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene. Oncogene 91739-1743. 4 K. Yoshino K. Nichigaki Y Husimi. 1991 Temperature sweep gel electrophoresis a simple method to detect point mutations. Nucleic Acids Res. 1931-53. 5 J. Bjorheim S. Lystad A. Lindblom U. Kressner et al. 1998 Mutation analysis of KRAS exon 1 comparing three different techniques temporal temperature gradient electrophoresis constant denaturant capillary electrophoresis and allele specific polymerase chain reaction. 1998 Mutat Res 403 103-112. 6 A.H Fanrleitner N. Kreuzinger G.G Kavka et al 2000 Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis in separating Escherichia Coli uidA amplicons differing in single base substitutions. Letters in Applied Microbiology 30 427- 431. SPECIFICATIONS Model Cat. TTGEK-2001 TTGE-K2401 of Cassettes of Gels 2 single cassettes 2 gels 2 dual cassettes 4 gels Gel Dimensions W x H cm 15 x 20 15 x 20 Applications DGGE TTGE CDGE DGGE TTGE CDGE Cipher TTGE Electrophoresis Systems p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 67 MUTATION DETECTION ORDERING INFORMATION CAT. DESCRIPTION CIPHER TTGE ELECTROPHORESIS SYSTEMS TTGEK-2001-110 Cipher TTGE Electrophoresis System 2-place 2 single cassettes 110V TTGEK-2001-220 Cipher TTGE Electrophoresis System 2-place 2 single cassettes 220V TTGEK-2401-110 Cipher TTGE Electrophoresis System 4-place 2 dual cassettes 110V TTGEK-2401-220 Cipher TTGE Electrophoresis System 4-place 2 dual cassettes 220V Related Products VGS0720RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 0.75mm thickness x 22cml VGS1020RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.0mm thickness x 22cml VGS1520RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.5mm thickness x 22cml VGC-0701-177 Comb for Perpendicular DGGE 0.75mm thick x 1 well VGC-1001-177 Comb for Perpendicular DGGE 1.0mm thick x 1 well VGC-1501-177 Comb for Perpendicular DGGE 1.5mm thick x 1 well VGS-0720R-177 Gel Wrap Spacer Set 0.75mm thick x 22cm with rounded bottom corners VGS-1020R-177 Gel Wrap Spacer Set 1.0mm thick x 22cm with rounded bottom corners VGS-1520R-177 Gel Wrap Spacer Set 1.5mm thick x 22cm with rounded bottom corners VGE-0720-177 Gel Wrap Gasket to cast one DGGE gel 0.75mm thick VGE-1020-177 Gel Wrap Gasket to cast one DGGE gel 1.0mm thick VGE-1520-177 Gel Wrap Gasket to cast one DGGE gel 1.5mm thick NGP-17720NR Gel Wrap Glass plate set back plate has rounded bottom corners 17.7cm w x 22 cm h NGP-17720DNR Gel Wrap Glass plate set Borosilicate back plate with rounded bottom corners 17.7cm w x 22 cm h NGP-17720-NR-LFP Gel Wrap Glass Plate Set both plates are Borosilicate. Back plate has rounded bottom corners 17.7cm w x 22 cm h GCC-404-177K Multiple Gel Casting Chamber Kit 4-place casts up to 4 each 15cm x 20cm gels VGS-0720C-177 Multiple Gel Casting Spacer Set 0.75mm thick x 22ml VGS-1020C-177 Multiple Gel Casting Spacer Set 1.0mm thick x 22ml VGS-1520C-177 Multiple Gel Casting Spacer Set 1.5mm thick x 22ml NGP-17720-N Multiple Gel Casting Chamber Glass Plate Set 7.7cmw x 22cmh NGP-17720-DN Multiple Gel Casting Chamber Glass Plate Set with Borosilicate back plate 7.7cmw x 22cmh NGP-17720-N-LFP Multiple Gel Casting Chamber Glass Plate Set both plates are Borosilicate 7.7cmw x 22cmh Gel Casting Options Option 1 Multi-Caster Kit Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough 4 sets notched glass plate sets 4 sets of spacers 4 combs polycarbonate separation sheets. See page 23 for assembly diagram or online video instructions http www.cbsscientific.cominstructionvideos.aspx. Option 2 Gel Wrap Casting System C.B.S. Scientific has developed a leak-proof gasket Gel Wrap that is a simplified method for casting perpendicular and vertical gels. Gel Wrap seals the glass plate sandwich for gel casting quickly and easily. Each TTGEK System comes with Gel Wrap gaskets and all components necessary to cast gels by this method. See page 23 for more detailed description or online video instructions httpwww.cbsscientific.com instructionvideos.aspx. Vertical Comb Options deeper wells overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716 19.1 16 0.75 5.2 55 VGC-0720 17.7 20 0.75 4.2 42 VGC-0722 17.7 22 0.75 4.35 25 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 55 VGC-1022 17.7 22 1.0 4.35 57 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1522 17.7 22 1.5 4.35 85 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 Vertical Comb Options short wells overall length 13.1cm Cat. Tooth depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716-177 10 16 0.75 5.2 29 VGC-0720-177 10 20 0.75 4.2 23 VGC-0722-177 10 22 0.75 4.35 25 VGC-0729-177 10 29 0.75 2.9 16 VGC-0730-177 10 30 0.75 2.8 16 VGC-1016-177 10 16 1.0 5.2 39 VGC-1020-177 10 20 1.0 4.2 32 VGC-1022-177 10 22 1.0 4.35 32 VGC-1029-177 10 29 1.0 2.9 22 VGC-1030-177 10 30 1.0 2.8 21 VGC-1516-177 10 16 1.5 5.2 58 VGC-1520-177 10 20 1.5 4.2 47 VGC-1522-177 10 22 1.5 4.35 49 VGC-1529-177 10 29 1.5 2.9 33 VGC-1530-177 10 30 1.5 2.8 32 Maximum loading volume is calculated by taking 75 of the total tooth volume. w w w. c b s s c i e n t i f i c . c o m68 MUTATION DETECTION Cipher DTSK Genetic Analysis Systems Cipher DTSK Genetic Analysis System 4-place Cat. DTSK-2401 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead EPS-300 X Power Supply High efficiency cooling coil Programmable HeaterStirrerBuffer Cycler with temperature ramping capability 2 acrylic dual gel cassettesupper reservoir with platinum electrodes Safety cover with electrical interlock Buffer siphon pump 8 combs 4 each 1 well and 4 each 16 well combs 8 sets of spacers 4 with injection ports for perpendicular gel casting 4 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 8 Gel Wrap gaskets White spring clamps Cipher DTSK Genetic Analysis System 2-place Cat. DTSK-2001 1 reinforced buffer tanklower reservoir with platinum electrodes and attached anode power lead High efficiency cooling coil EPS-300 X Power Supply Programmable HeaterStirrerBuffer Cycler with temperature ramping capability 2 acrylic gel cassettesupper reservoir with platinum electrodes Safety cover with electrical interlock Buffer siphon pump 4 combs 2 each 1 well and 2 each 16 well combs 4 sets of spacers 2 with injection ports for perpendicular gel casting 2 sets of glass plates MPP-100 Mini-peristaltic pump for gradient gel formation GM-40 Gradient maker 20mls per side 4 Gel Wrap gaskets White spring clamps APPLICATIONS Detecting single base DNA changesmutations Detecting polymorphisms in double stranded DNA Determining DNA fragment melting points Single Strand Conformational Polymorphism SSCP Extremely accurate and highly reliable Mutation Detection Systems for DGGE TTGE CDGE and SSCP FEATURES Cipher DTSK Genetic Analysis Systems are equipped with a large diameter cooling coil in the anode buffer chamber to accommodate SSCP techniques. The cooling coil is connected to an external refrigerated water bath chiller. The Cipher Instrument automatically mixes the buffer and regulates the overall temperature holding the buffer temperature within 0.2C of the set temperature assuring consistent intra- and inter-gel conditions. Internal peristaltic pump for buffer cycling This system lends itself well to the use of fluorescence based assays whose images can be captured on a fluorimeter directly through optional Borosilicate glass plate sets. A mini-peristaltic pump MPP-100 used in conjunction with the GM- 40 gradient maker produces accurate and consistent gradient gels Single or dual gel cassettes are offered which have been designed to eliminate the need of agarose plugs. Buffer tank dimensions have been reduced to use bench space more efficiently Safety cover has electrical interlock for protection and to help maintain temperature and reduce evaporation To avoid any incompatibility issues the EPS-300 X CE Power Supply is included because its specifications are suited to run all DGGE and TTGE applications. Simplified casting method using Gel Wrap gasket for both perpendicular and vertical gels DGGE AND CDGE Denaturing Gradient Gel Electrophoresis DGGE is a powerful genetic analysis technique that can be used for detecting single base changes and polymorphisms in genomic cloned and PCR amplified DNA. Two of the most valuable uses for DGGE in human and microbial genetics are in directly detecting single base changes that cause disease and in detecting polymorphisms with DNA probes for genetic-linkage analysis. In DGGE conformational transitions of multiple nucleic acid complexes are induced by an increasing concentration of solvent UreaFormamide at a constant temperature. Clinical applications of DGGE include a rapid and effective method for screening samples for genetic mutations and variants. Also DNA fragment melting points can be determined using perpendicular DGGE. In contrast to DGGE CDGE Constant Denaturant Gel Electrophoresis uses a single solvent percentage to induce partial melting of DNA fragments as they enter the gel. The disadvantage of CDGE is that only a single melting domain can be interrogated. TTGE Destabilization of nucleic acid complexes can be studied using acrylamide gels that contain a uniform solvent concentration as in CDGE but with an increasing temperature gradient 1. Since the temperature of the entire gel is uniformly raised over a period of time this technique has been termed TTGE or Temporal Temperature Gradient Electrophoresis 2. This tech- nique incorporates many improvements over DGGECDGE especially when studying multiple melting domains 3. SSCP Single Strand Conformational Polymorphism SSCP reveals differences in electrophoretic mobility between normal and mutant single strands of DNA 4. In SSCP normal and mutant duplex DNA are denatured to form single stranded molecules of equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing form different three-dimensional structures. These structures differ in electrophoretic mobility and can be separated on a chilled non-denaturing polyacrylamide gel. The electrophoretic mobility of these conformers change depending on temperature and buffer ionic strength. For accurate characterization of mutations within these re-folded single strands it is essential that the buffer temperature be tightly controlled within each electrophoresis procedure usually between 4-15C. If the gel temperature is not precisely controlled the resolution will suffer because of the loss of intrastrand base pairing and change in the overall shape of the 3-dimensional conformer. REFERENCES See page 66. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 69 MUTATION DETECTION SPECIFICATIONS Model Cat. DTSK-2001 DTSK-2401 of Cassettes of Gels 2 single cassettes 2 gels 2 dual cassettes 4 gels Gel Dimensions 15cmw x 20cmh 15cmw x 20cmh Applications DGGE TTGE CDGE SSCP DGGE TTGE CDGE SSCP Gel Casting Options Option 1 Multi-Caster Kit Option 2 Gel Wrap Casting System Multi-Caster Kits 4-place Cat. GCC-404-177K Multi-Caster Chamber with lid silicone gasket 6 stainless steel thumbscrews backfill port connecting to central filling trough 4 sets notched glass plate sets 4 sets of spacers choice of 0.75mm 1.0mm or 1.5mm thickness 4 combs Polycarbonate separation sheets C.B.S. Scientific has developed a revolutionary leak-proof gasket Gel Wrap that is a simplified method for casting perpendicular and vertical gels. Gel Wrap seals the glass plate sandwich for gel casting quickly and easily. Each DTSK System comes with Gel Wrap gaskets and all components necessary to cast gels by this method. See page 23 for more detailed description. ORDERING INFORMATION CAT. DESCRIPTION CIPHER DTSK GENETIC ANALYSIS SYSTEMS DTSK-2001 Cipher DTSK Genetic Analysis System 2-place 2 single cassettes Specify 110V or 220V DTSK-2401 Cipher DTSK Genetic Analysis System 4-place 2 dual cassettes Specify 110V or 220V Related Products VGS0720RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 0.75mm thickness x 22cml VGS1020RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.0mm thickness x 22cml VGS1520RC-177 Spacer Set for Perpendicular DGGE standard spacer injection port spacer 1.5mm thickness x 22cml VGC-0701-177 Comb for Perpendicular DGGE 0.75mm thick x 1 well VGC-1001-177 Comb for Perpendicular DGGE 1.0mm thick x 1 well VGC-1501-177 Comb for Perpendicular DGGE 1.5mm thick x 1 well VGS-0720R-177 Gel Wrap Spacer Set 0.75mm thick x 22cm with rounded bottom corners VGS-1020R-177 Gel Wrap Spacer Set 1.0mm thick x 22cm with rounded bottom corners VGS-1520R-177 Gel Wrap Spacer Set 1.5mm thick x 22cm with rounded bottom corners VGE-0720-177 Gel Wrap Gasket to cast one DGGE gel 0.75mm thick VGE-1020-177 Gel Wrap Gasket to cast one DGGE gel 1.0mm thick VGE-1520-177 Gel Wrap Gasket to cast one DGGE gel 1.5mm thick NGP-17720NR Gel Wrap Glass plate set back plate has rounded bottom corners 17.7cm w x 22 cm h NGP-17720DNR Gel Wrap Glass plate set Borosilicate back plate with rounded bottom corners 17.7cm w x 22 cm h NGP-17720-N-LPF Gel Wrap Glass Plate Set both plates are Borosilicate. Back plate has rounded bottom corners 17.7cm w x 22 cm h GCC-404-177K Multiple Gel Casting Chamber Kit 4-place VGS-0720C-177 Multiple Gel Casting Spacer Set 0.75mm thick x 22ml VGS-1020C-177 Multiple Gel Casting Spacer Set 1.0mm thick x 22ml VGS-1520C-177 Multiple Gel Casting Spacer Set 1.5mm thick x 22ml NGP-17720-N Multiple Gel Casting Chamber Glass Plate Set 7.7cmw x 22cmh NGP-17720-DN Multiple Gel Casting Chamber Glass Plate Set with Borosilicate back plate 7.7cmw x 22cmh NGP-17720-N-LPF Multiple Gel Casting Chamber Glass Plate Set both plates are Borosilicate 7.7cmw x 22cmh Vertical Comb Options short wells overall length 13.1cm Cat. Tooth depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716-177 10 16 0.75 5.2 29 VGC-0720-177 10 20 0.75 4.2 23 VGC-0722-177 10 22 0.75 4.35 25 VGC-0729-177 10 29 0.75 2.9 16 VGC-0730-177 10 30 0.75 2.8 16 VGC-1016-177 10 16 1.0 5.2 39 VGC-1020-177 10 20 1.0 4.2 32 VGC-1022-177 10 22 1.0 4.35 32 VGC-1029-177 10 29 1.0 2.9 22 VGC-1030-177 10 30 1.0 2.8 21 VGC-1516-177 10 16 1.5 5.2 58 VGC-1520-177 10 20 1.5 4.2 47 VGC-1522-177 10 22 1.5 4.35 49 VGC-1529-177 10 29 1.5 2.9 33 VGC-1530-177 10 30 1.5 2.8 32 Vertical Comb Options deeper wells overall length 13.1cm Cat. Tooth Depth mm of Teeth Thickness of Teeth Width of Teeth Recommended Max. Sample Vol.Well l VGC-0716 19.1 16 0.75 5.2 55 VGC-0720 17.7 20 0.75 4.2 42 VGC-0722 17.7 22 0.75 4.35 25 VGC-0729 17.7 29 0.75 2.9 29 VGC-0730 17.7 30 0.75 2.8 28 VGC-1016 19.1 16 1.0 5.2 74 VGC-1020 19.1 20 1.0 4.2 55 VGC-1022 17.7 22 1.0 4.35 57 VGC-1029 17.7 29 1.0 2.9 38 VGC-1030 17.7 30 1.0 2.8 37 VGC-1516 19.1 16 1.5 5.2 110 VGC-1520 17.7 20 1.5 4.2 83 VGC-1522 17.7 22 1.5 4.35 85 VGC-1529 17.7 29 1.5 2.9 58 VGC-1530 17.7 30 1.5 2.8 56 Maximum loading volume is calculated by taking 75 of the total tooth volume. w w w. c b s s c i e n t i f i c . c o m70 SEQUENCING C.B.S. Scientifics Dual Adjustable Sequencers are available in two widths 20cm or 33cm. Sequencing gel pathlength for either width can be adjusted from 22cm up to 62cm. The upper reservoir is divided in half allowing the researcher to run identical or independent buffer conditions on either side. The rotating base allows the unit to rotate 180 providing easy access to either side. Accessory aluminum cooling plates and accessory bar clamps shown in photo must be ordered separately. Additional accessory choices are available to accommodate Large Format Protein Gel applications. SG-500-33 Adjustable Height Sequencer shown with accessory aluminum plate and bar clamp C.B.S. Scientifics Adjustable Height Sequencers are available in two widths 20cm or 33cm. Sequencing gel pathlength for either width can be adjusted from 22cm up to 62cm. The upper buffer reservoir rides on three 1 diameter delrin rods and is secured at the desired height by three stainless steel thumbscrews. These rods are arranged in a triangular configuration on a 12 thick acrylic base to add strength and stability to the whole system. Unit can be easily disassembled into components for compact storage. Additional accessory choices are available to accommodate Large Format Protein Gel applications. Dual Adjustable Height Sequencers DASG-400-20 DASG-500-20 DASG-600-20 DASG-400-33 DASG-500-33 DASG-600-33 Complete System Dual vertical electrophoresis cell with rotating leveling base with bubble level Safety covers with power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes Divided upper reservoir 2 combs 2 sets glass plates 2 sets of spacers 1 bottle of Gel Repel 1 roll gel sealing tape 2 wedge plate separators White spring clamps Buffer drainage ports Adjustable Height Sequencers Single SG-400-20 SG-500-20 SG-600-20 SG-400-33 SG-500-33 SG-600-33 Complete System Single vertical electrophoresis cell with safety covers and power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes Leveling base with bubble level 1 comb 1 set glass plates 1 set of spacers 1 bottle of Gel Repel 1 roll gel sealing tape 1 wedge plate separator White spring clamps Buffer drainage ports DASG-500-33 Dual Adjustable Height Sequencer shown with accessory aluminum plate and bar clamp Adjustable Height Sequencers APPLICATIONS DNA Sequencing Mutation Detection Genotyping Minisatellites Differential Display Large Format SDS PAGE RNase protection DNA footprinting DNA fingerprinting MicrosatellitesSSTR Unsurpassed versatility - designed to adapt to changing methodologies p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 71 SEQUENCING FEATURES Can run short gels for oligonucleotide protein or preparative DNA separations as well as long gels for analytical sequencing on same unit by ordering appropriate accessories. Both Single and Dual Adjustable systems are offered in two widths 20cm and 33cm and provide flexibility to run gel lengths of 22cm 32cm 42cm 52cm or 62cm. A wide selection of precision-machined combs and spacers is available including specially configured sharktooth combs for multi-channel pipettor loading. Compressible long-lasting silicone gasket on face of unit provides leak- proof seal when glass plates are clamped in place. The rotating base of the Dual Adjustable Systems allow easy access to either side of unit. A removable peg locks the base of the unit into place providing stability for either 180 rotation. Simply load one side remove peg rotate replace peg and continue loading alternate side. Both upper and lower reservoirs have drains with tubing adapters for easy disposal of buffer. Bottom buffer reservoir conveniently detaches from base of unit for ease in cleaning and disposal of buffer. Accessory anodized aluminum cooling plates can be ordered to clamp onto glass plates to evenly distribute the heat generated during electrophoresis Optional acrylic bar clamp provides equal pressure along entire width of glass platecomb sandwich eliminating capillary leakage of sample while loading sharktooth combs. Thicker accessory choices are available to accommodate Large Format Protein Gel applications. Adjustable height sequencers at heights of 42cm 52cm and 62cm Acrylic Bar Clamp ORDERING INFORMATION CAT. DESCRIPTION Adjustable Height Sequencers SG-400-20 Adjustable Height Sequencer Complete System 20cmw x 22cm - 42cmh capability SG-500-20 Adjustable Height Sequencer Complete System 20cmw x 22cm - 52cmh capability SG-600-20 Adjustable Height Sequencer Complete System 20cmw x 22cm - 62cmh capability SG-400-33 Adjustable Height Sequencer Complete System 33cmw x 22cm - 42cmh capability SG-500-33 Adjustable Height Sequencer Complete System 33cmw x 22cm - 52cmh capability SG-600-33 Adjustable Height Sequencer Complete System 33cmw x 22cm - 62cmh capability DASG-400-20 Dual Adjustable Height Sequencer Complete System 20cmw x 22cm - 42cmh capability DASG-500-20 Dual Adjustable Height Sequencer Complete System 20cmw x 22cm - 52cmh capability DASG-600-20 Dual Adjustable Height Sequencer Complete System 20cmw x 22cm - 62cmh capability DASG-400-33 Dual Adjustable Height Sequencer Complete System 33cmw x 22cm - 42cmh capability DASG-500-33 Dual Adjustable Height Sequencer Complete System 33cmw x 22cm - 52cmh capability DASG-600-33 Dual Adjustable Height Sequencer Complete System 33cmw x 22cm - 62cmh capability EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA See Sequencing Accessories on pages 74-77 Product List or website for complete listing of catalog numbers and ordering options w w w. c b s s c i e n t i f i c . c o m72 SEQUENCING C.B.S. Scientifics Dual Dedicated Height Sequencer has all of the convenience and safety features of our Dedicated Height Sequencer but you can run twice as many samples using less buffer and the amount of valuable bench space. The Dual Dedicated height Sequencer is available in 20cm and 33cm widths and with height capabilities of 42cm 52cm or 62cm. A rotating base allows the unit to rotate 180 providing easy access to either side. The upper reservoir is divided in half allowing the researcher to run identical or independent buffer conditions on either side. This sequencer features 2 bar clamps 2 built in spring-mounted epoxy- coated aluminum cooling plates safety interlocking leads and independent detachable lower buffer reservoirs. DH-500-33 Dedicated Height Sequencer 52cm height C.B.S. Scientifics Dedicated Height Sequencers are available in two widths 20cm or 33cm and can be ordered in heights of 42cm 52cm or 62cm. These sequencers feature safety-interlocking lids detachable upper and lower reservoirs and a built-in epoxy-coated aluminum heat dispersion plate. A clear acrylic bar clamp is provided with each unit. Additional accessory choices are available to accommodate Large Format Protein Gel applications. Dual Dedicated Height Sequencers DDH-400-20 DDH-500-20 DDH-600-20 DDH-400-33 DDH-500-33 DDH-600-33 Complete System Dual vertical electrophoresis cell with 2 built-in epoxy-coated spring mounted aluminum cooling plates divided upper reservoir and rotating leveling base with bubble level 2 Bar clamps Safety covers with power leads Leak proof silicone gasket on upper reservoir to which the glass plates are clamped Platinum electrodes Divided upper reservoir 2 combs 2 sets glass plates 2 sets of spacers 1 bottle of Gel Repel 1 roll gel sealing tape 2 wedge plate separators White spring clamps Buffer drainage ports Single Dedicated Height Sequencers DH-400-20 DH-500-20 DH-600-20 DH-400-33 DH-500-33 DH-600-33 Complete System Single vertical electrophoresis cell with built-in epoxy-coated spring mounted aluminum cooling plate interlocking safety covers and power leads Bar clamp Safety covers with attached leads Leak proof silicone gasket on upper reservoir Platinum electrodes Leveling base with bubble level 1 comb 1 set glass plates 1 set of spacers 1 bottle of Gel Repel 1 roll gel sealing tape 1 wedge plate separator White spring clamps Buffer drainage ports DDH-500-33 Dual Dedicated Height Sequencer 52cm height Dedicated Height Sequencers Single or Dual Sequencers offered in 3 heights and 2 widths APPLICATIONS DNA Sequencing Mutation Detection Genotyping Minisatellites Differential Display Large Format SDS PAGE RNase protection DNA footprinting DNA fingerprinting MicrosatellitesSSTR p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 73 SEQUENCING FEATURES Both Single and Dual systems are offered in two widths 20cm and 33cmm and are available in heights of 42cm 52cm or 62cm. The Dual Dedicated Height Sequencers upper reservoir is divided in half allowing the researcher to run identical or independent buffer conditions on either side. This feature is also convenient for the researcher who occasionally wants to run just one side of the unit. Convenient built-in epoxy-coated aluminum plates help to evenly distribute the heat generated during electrophoresis eliminating the smile effect. These heat dispersion cooling plates are anodized for electrical isolation and white epoxy-coated for easy visualization of tracking dyes. These aluminum plates are also spring-mounted to reduce stress when clamping on sequencing glass plates and to help provide a close and even contact with the back glass plate. A wide selection of precision-machined combs and spacers is available including specially configured sharktooth combs for multi-channel pipettor loading. Compressible long-lasting silicone gasket on face of unit provides leak- proof seal when glass plates are clamped in place. The rotating base of the Dual Dedicated Height Systems allow easy access to either side of unit. A removable peg locks the base of the unit into place providing stability for either 180 rotation. Simply load one side remove peg rotate replace peg and continue loading alternate side. Both upper and lower reservoirs have drains with tubing adapters for easy disposal of buffer. Bottom buffer reservoir conveniently detaches from base of unit for ease in cleaning and disposal of buffer. Acrylic bar clamp provides equal pressure along entire width of glass platecomb sandwich eliminating capillary leakage of sample while loading sharktooth combs. Safety covers are extended over glass plate sandwich on upper reservoir for maximum protection. Thicker accessory choices are available to accommodate Large Format Protein Gel applications. Acrylic Bar Clamp ORDERING INFORMATION CAT. DESCRIPTION Dedicated Height Sequencers DH-400-20 Dedicated Height Sequencer Complete System 20cmw x 42cmh DH-500-20 Dedicated Height Sequencer Complete System 20cmw x 52cmh DH-600-20 Dedicated Height Sequencer Complete System 20cmw x 62cmh DH-400-33 Dedicated Height Sequencer Complete System 33cmw x 42cmh DH-500-33 Dedicated Height Sequencer Complete System 33cmw x 52cmh DH-600-33 Dedicated Height Sequencer Complete System 33cmw x 62cmh DDH-400-20 Dual Dedicated Height Sequencer Complete System 20cmw x 42cmh DDH-500-20 Dual Dedicated Height Sequencer Complete System 20cmw x 52cmh DDH-600-20 Dual Dedicated Height Sequencer Complete System 20cmw x 62cmh DDH-400-33 Dual Dedicated Height Sequencer Complete System 33cmw x 42cmh DDH-500-33 Dual Dedicated Height Sequencer Complete System 33cmw x 52cmh DDH-600-33 Dual Dedicated Height Sequencer Complete System 33cmw x 62cmh EPS-3000P-II High Voltage Power Supply CE 20-3000V 1-400mA See Sequencing Accessories on pages 74-77 Product List or website for complete listing of catalog numbers and ordering options w w w. c b s s c i e n t i f i c . c o m74 SEQUENCING Printed Sharktooth Combs The C.B.S. Scientific sharktooth combs are printed with well numbers vertical loading indicator lines catalog number for easy identification and horizontal lines to indicate the optimal casting well depth. The numbered wells allow a convenient method to keep track of which sample goes into which well. Each comb also features oval finger slots for easy removal. These combs are standard equipment with all CBS Scientific sequencing kits. All sharktooth combs are multi-channel pipettor compatible. Sharktooth combs provide for side by side sample application making sequencing analysis easier by eliminating the space between sample wells. This method requires the formation of a single well into which the points of the sharktooth combs are inserted. A single well can be formed by either using a 1-well casting comb or the back straight edge of the sharktooth combs. See charts at top of page 75. Rectangular Well Combs for Sequencing Rectangular combs are precision-machined polycarbonate and are available in a wide variety of sizes for both 20cm and 33cm wide units. See charts on bottom of page 75. Large Format Combs for Protein Applications PTFE precision-machined combs are available in 20cm and 33cm widths for preparative or protein gels. See charts on page 76. Combs for Multi-Channel Pipettor Loading C.B.S. Scientific has designed polycarbonate rectangular tooth and sharktooth combs with microtitre plate spacing for multi-channel pipettor loading. Rectangular combs with this feature are the 17 and 35 well combs for the 20cm wide units and 31 and 63 well combs for the 33cm wide units. The sharktooth combs that can be loaded with a multi-channel pipettor are the 2.25mm 3.0mm and 4.5mm point to point combs. The 2.25mm point to point combs correspond to our 126 well comb for the 33cm wide sequencers or 70 well for the 20cm wide sequencer and a multi-channel pipettor would load every 4th well. The 3.0mm point to point combs correspond to 94 well for 33cm wide sequencers or 52 well for the 20cm wide and a multi-channel pipettor would load every 3rd well. The 4.5mm point to point combs correspond to our 62 well comb for the 33cm wide sequencers or our 34 well comb for the 20cm wide units and a multi-channel pipettor would load every 2nd well. See diagram a right. Sequencing Accessories p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 75 SEQUENCING SHARKTOOTH COMB OPTIONS for 20cm wide Sequencing units overall length 16cm depth of rectangular tooth 10mm Cat. Comb Type of Teeth Thickness of Teeth mm Point to Point mm Maximum Well Volume l SK20-0201 casting comb 1 0.2 - 317 SK20-0234 sharktooth 34 0.2 4.5 3.3 SK20-0252 sharktooth 52 0.2 3.0 2.0 SK20-0270 sharktooth 70 0.2 2.25 1.4 SK20-0401 casting comb 1 0.4 - 634 SK20-0434 sharktooth 34 0.4 4.5 6.6 SK20-0452 sharktooth 52 0.4 3.0 4.0 SK20-0470 sharktooth 70 0.4 2.25 2.8 RECTANGULAR POLYCARBONATE COMB OPTIONS - 20cm wide overall length 15.87cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG20-0208 9.5 8 0.2 17.7 25.2 SG20-0212 9.5 12 0.2 11 15.6 SG20-0214 9.5 14 0.2 9.00 12.7 SG20-0216 9.5 16 0.2 7.6 10.8 SG20-0217 9.5 17 0.2 6.63 9.4 SG20-0220 9.5 20 0.2 5.66 7.9 SG20-0228 9.5 28 0.2 4 5.7 SG20-0232 9.5 32 0.2 3.4 4.8 SG20-0235 9.5 35 0.2 2.9 3.7 SG20-0408 9.5 8 0.4 17.7 50 SG20-0412 9.5 12 0.4 11 31.2 SG20-0414 9.5 14 0.4 9.0 25.5 SG20-0416 9.5 16 0.4 7.6 21 SG20-0417 9.5 17 0.4 6.63 18.7 SG20-0420 9.5 20 0.4 5.66 15.9 SG20-0428 9.5 28 0.4 4 11.4 SG20-0432 9.5 32 0.4 3.4 9.6 SG20-0435 9.5 35 0.4 2.9 7.5 SHARKTOOTH COMB OPTIONS for 33cm wide Sequencing units overall length 30cm depth of rectangular tooth 10mm Cat. Comb Type of Teeth Thickness of Teeth mm Point to Point mm Maximum Well Volume l SK33-0201 casting comb 1 0.2 - 539.6 SK33-0262 sharktooth 62 0.2 4.5 3.3 SK33-0294 sharktooth 94 0.2 3.0 2.0 SK33-02126 sharktooth 126 0.2 2.25 1.71 SK33-0401 casting comb 1 0.4 - 1079.2 SK33-0462 sharktooth 52 0.4 4.5 6.6 SK33-0494 sharktooth 94 0.4 3.0 4.0 SK33-04126 sharktooth 126 0.4 2.25 3.4 multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. RECTANGULAR POLYCARBONATE COMB OPTIONS - 33cm wide overall length 30cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG33-0208 9.5 8 0.2 33.4 47.6 SG33-0216 9.5 16 0.2 15.5 22 SG33-0224 9.5 24 0.2 9.5 13.5 SG33-0231 9.5 31 0.2 5.8 8.3 SG33-0234 9.5 34 0.2 6.0 8.5 SG33-0250 9.5 50 0.2 4.1 5.8 SG33-0260 9.5 60 0.2 3.1 4.3 SG33-0263 9.5 63 0.2 2.9 4.1 SG33-0408 9.5 8 0.4 33.4 95.2 SG33-0416 9.5 16 0.4 15.5 44.3 SG33-0424 9.5 24 0.4 9.5 27 SG33-0431 9.5 31 0.4 15.8 15 SG33-0434 9.5 34 0.4 6.0 16.5 SG33-0450 9.5 50 0.4 4.1 11.6 SG33-0460 9.5 60 0.4 3.1 8.7 SG33-0463 9.5 63 0.4 2.9 8.2 Sharktooth Combs Rectangular Combs for Sequencing multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. w w w. c b s s c i e n t i f i c . c o m76 SEQUENCING Sequencing Accessories Large Format Combs For Protein Applications LARGE FORMAT COMB OPTIONS - 33cm wide overall length 28.41cm Cat. Tooth Depth mm of Teeth Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG33-0703 19.05 3 0.75 93.1 1330.4 SG33-0705 19.05 5 0.75 54.3 775.5 SG33-0706 19.05 6 0.75 45.4 648 SG33-0708 19.05 8 0.75 33.4 477.5 SG33-0712 19.05 12 0.75 21.5 307.1 SG33-0716 19.05 16 0.75 15.5 221.7 SG33-0724 19.05 24 0.75 9.55 136.4 SG33-0731 17.45 31 0.75 7.82 76.2 SG33-0734 17.45 34 0.75 6.05 79.2 SG33-0750 17.45 50 0.75 4.14 54.3 SG33-0760 17.45 60 0.75 3.18 41.7 SG33-0763 17.45 63 0.75 2.92 38.2 SG33-1003 19.05 3 1.0 93.1 1330.4 SG33-1005 19.05 5 1.0 54.3 775.5 SG33-1006 19.05 6 1.0 45.4 648.1 SG33-1008 19.05 8 1.0 33.4 477.6 SG33-1012 19.05 12 1.0 21.5 307.1 SG33-1016 19.05 16 1.0 15.5 221.7 SG33-1024 19.05 24 1.0 9.55 136.4 SG33-1031 17.45 31 1.0 7.82 76.2 SG33-1034 17.45 34 1.0 6.05 79.2 SG33-1050 17.45 50 1.0 4.14 54.3 SG33-1060 17.45 60 1.0 3.18 41.7 SG33-1063 17.45 63 1.0 2.92 28.6 SG33-1503 19.05 3 1.5 93.1 1995 SG33-1505 19.05 5 1.5 54.3 1163 SG33-1506 19.05 6 1.5 45.4 972.1 SG33-1508 19.05 8 1.5 33.4 671.4 SG33-1512 19.05 12 1.5 21.5 460.5 SG33-1516 19.05 16 1.5 15.5 332.5 SG33-1524 19.05 24 1.5 9.55 204.6 SG33-1531 17.45 31 1.5 7.82 114.3 SG33-1534 17.45 34 1.5 6.05 118.8 SG33-1550 17.45 50 1.5 4.14 81.4 SG33-1560 17.45 60 1.5 3.18 62.5 SG33-1563 17.45 63 1.5 2.92 42.9 SG33-2003 19.05 3 2.0 93.1 2660 SG33-2005 19.05 5 2.0 54.3 1551 SG33-2006 19.05 6 2.0 45.4 1296 SG33-2008 19.05 8 2.0 33.4 955.2 SG33-2012 19.05 12 2.0 21.5 614.2 SG33-2016 19.05 16 2.0 15.5 442 SG33-2024 17.45 24 2.0 9.55 272.8 SG33-2031 17.45 31 2.0 7.82 152.4 SG33-2034 17.45 34 2.0 6.05 158.4 SG33-2050 17.45 50 2.0 4.14 108.6 SG33-2060 17.45 60 2.0 3.18 83.4 SG33-2063 17.45 63 2.0 2.92 57.2 Note 3.0mm combs and spacers are also available for the 33 cm wide Sequencers. Please consult website for catalog numbers. Multi-channel pipettor compatible Maximum loading volume is calculated by taking 75 of the total tooth volume. LARGE FORMAT COMB OPTIONS - 20cm wide material PTFE overall length 15.87cm Cat. of Teeth Tooth Depth mm Thickness of Teeth mm Width of Teeth mm Recommended Max. Sample Vol.Well l SG20-0708 8 19.05 0.75 17.7 95 SG20-0712 12 19.05 0.75 11 58 SG20-0717 17 19.05 0.75 6.63 35 SG20-0720 20 19.05 0.75 5.66 30 SG20-0728 28 17.45 0.75 4 22 SG20-0732 32 17.45 0.75 3.4 18 SG20-0735 35 17.45 0.75 2.9 14 SG20-1008 8 19.05 1.0 17.7 126 SG20-1012 12 19.05 1.0 11 78 SG20-1017 17 19.05 1.0 6.63 50 SG20-1020 20 19.05 1.0 5.66 40 SG20-1028 28 17.45 1.0 4 29 SG20-1032 32 17.45 1.0 3.4 24 SG20-1035 35 17.45 1.0 2.9 19 SG20-1508 8 19.05 1.5 17.7 190 SG20-1512 12 19.05 1.5 11 117 SG20-1517 17 19.05 1.5 6.63 71 SG20-1520 20 19.05 1.5 5.66 60 SG20-1528 28 17.45 1.5 4 43 SG20-1532 32 17.45 1.5 3.4 36 SG20-1535 35 17.45 1.5 2.9 29 SG20-2008 8 19.05 2.0 17.7 252 SG20-2012 12 19.05 2.0 11 156 SG20-2017 17 19.05 2.0 6.63 94 SG20-2020 20 19.05 2.0 5.66 80 SG20-2028 28 17.45 2.0 4 57 SG20-2032 32 17.45 2.0 3.4 48 SG20-2035 35 17.45 2.0 2.9 38 SG20-3008 8 19.05 3.0 17.7 378 SG20-3012 12 19.05 3.0 11 234 SG20-3017 17 19.05 3.0 6.63 140 SG20-3020 20 19.05 3.0 5.66 119 SG20-3028 28 17.45 3.0 4 86 SG20-3032 32 17.45 3.0 3.4 72 SG20-3035 35 17.45 3.0 2.9 56 p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 77 SEQUENCING Spacer Sets Sequencing spacer sets for all sequencer widths are made from polycarbonate. Polycarbonate spacers are compatible with polycarbonate or Valox combs and are available in thicknesses of 0.2mm and 0.4mm. Spacers are offered in 22cm 32cm 42cm 52cm 62cm 82cm and 102cm. PVC spacers sets and PTFE combs for preparative or SDS Page protein gels can be ordered with thicknesses of 0.75mm 1.0mm 1.5mm 2.0mm or 3.0mm. All spacer sets are manufactured to close tolerances. Glass Plates Glass plates sets can be ordered in lengths in 22cm 32cm 42cm 52cm 62cm 82cm or 102cm. Standard notched plate sets provided with the kits are made from sodalime glass. All glass plate edges are ground and polished for safety and durability. Unnotched plate sets with detached rubber ears can also be ordered. Custom sizes can be ordered using the custom order form. CBS Scientific offer two options for glass material standard sodalime glass plate sets or Borosilicate glass plate sets. Borosilicate glass plates Automated Sequencer grade highest quality very flat very low fluorescence background. Fluorescent image can be captured directly through the glass using a fluorimeter. Low Fluorescence Glass Plates Borosilicate or low-fluorescence glass plates sets are 5mm in thickness. Unnotched plate sets with detached rubber ears can also be ordered. All glass plate edges are ground and polished for safety and durability. Glass Plate Racks This convenient 12 place glass plate drying andor storage rack is constructed of high strength structural ABS with hand supports for ease in carrying from bench to sink. Shown at right Cat. GRH-100. Buffer Drainage There are three different options for buffer drainage A Ball Valve with simple on-off lever that can be attached to tubing and greatly simplifies buffer disposal Cat. BVD-100. The Ball Valve can be ordered to replace the standard tubing adapters C supplied with all CBS Scientific Sequencing Systems. B Quick disconnect tubing coupling cat. QDC-P body and insert set. This coupling features an automatic shut-off valve and is another alternative to the standard tubing adapters. The quick-disconnect coupling inset with shut-off valve threads into the buffer reservoirs while the coupling body attaches to the drainage tubing. To drain reservoir simply snap coupling body onto coupling insert and the valve will open. When the coupling is disconnected the valve automatically closes. C Standard tubing adapter supplied with all CBS Sequencing Systems. Can be replaced with option A or B. Gel Slider - Adjustable Width Sequencing Gel Caster The Gel Slider greatly simplifies the pouring of problem-free sequencing gels as thin as 0.05mm and can be adjusted to cast gels of many dimensions. This sliding plate technique can be accomplished in a matter of minutes and eliminates the time consuming and often unreliable use of yellow tape clamps or plasticine. Pouring a gel from the top of the gel plate sandwich can often result in trapped air bubbles or acrylamide leakage. The sliding plate technique solves these problems by utilizing capillary action to advance the gel boundary resulting in bubble-free gels. Both preparation and clean-up are greatly reduced. GRH-100 A B C ORDERING INFORMATION CAT. DESCRIPTION GEL SLIDER GSL-1001 Gel Slider Gel Caster cast plates 20cm-38cmw x 22-42cml GSL-2001 Gel Slider Gel Caster cast plates 20cm-38cmw x 22-62cml w w w. c b s s c i e n t i f i c . c o m78 LAB EQUIPMENT Pipette Racks Four tier pipette racks are available for convenient storage of disposable plastic pipettes. These racks are ideal for storage of pipettes inside the PCR Workstation or on the benchtop. Depending on user preference racks may be ordered for the right or left side work area. Removal of pipettes from the left is specified by an L at the end of the catalog number while removal from the right is specified by the letter R. QuickTrap Vacuum Protection System QuickTrap Systems are for the aspiration of standard radioactive or pathogenic cell culture supernatants. Housed in acrylic cabinets for protection from beta radioisotopes. Primary and secondary traps Tygon vacuum tubing with in-line ABS quick-disconnect fittings Hydrophobic PTFE filter with PP housing stops overflow into vacuum source Heavy-duty Nalgene polyethylene bottle liquid storage containers chemically resistant and autoclavable. Closures have TPE gaskets. All plastic construction eliminates implosion hazard Tygon is a registered trademark of Norton Plastics. Nalgene is a registered trademark of Nalge Inc.. QT-100-5 QT-100-2 PR-048-4L PR-048-4R Laboratory Equipment Two sizes of high strength ABS glass plate racks for either mini-gel plates shown at left or large format gelssequencing glass plates shown at right. Larger size features hand supports for carrying large plates from bench to sink. Glass Plate Racks GR-200-12B GRH-100 ORDERING INFORMATION CAT. DESCRIPTION GLASS PLATE RACKS GR-200-12B Mini Glass Plate Rack Holds 12 glass plates GR-200-20B Mini Glass Plate Rack Holds 20 glass plates GRH-100 Glass Plate Rack Holds 12 glass plates ORDERING INFORMATION CAT. DESCRIPTION QUICKTRAP VACUUM PROTECTION SYSTEM QT-100-2 QuickTrap System 2 liter QT-100-5 QuickTrap System 5 liter ORDERING INFORMATION CAT. DESCRIPTION PIPETTE RACKS PR-048-4L 4 Tier Pipette Rack pipettes are lifted from the left. PR-048-4R 4 Tier Pipette Rack pipettes are lifted from the right FREEZEBEE The FREEZEBEE works in conjunction with a standard size ice bucket to keep your samples cold and to prevent them from being lost beneath the ice bed. Different size holes in the FREEZEBEE permit a wide array of sample tubes to be stored in a convenient way. Accommodates 48 each 0.5ml microcentrifuge tubes 47 each 1.5ml microcentrifuge tubes 8 each 15ml conical tubes and 6 each of the 50ml conical tubes. ORDERING INFORMATION CAT. DESCRIPTION FREEZEBEE FZB-109 FREEZEBEE Microfuge Tube Racks Two types of microfuge tube racks are offered - the 96-Place Flip Racks and 80-Place Racks. See ordering information on page 81. p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 79 LAB EQUIPMENT C.B.S. Scientific offers 8 different sizes of linear gradient makers with side outlets. Gradient flow is controlled by a PTFE valve centered between the two chambers. The outflow channel is fitted with a male luer adapter which can be used with any needle or luer valve. The smaller gradient makers have a support rod for attachment to a ring stand. Available in sizes of 10ml 20ml 50mls 75mls 100mls 250mls 500mls and 1000mls per side. This is a variable flow self-priming Mini-Peristaltic Pump for precise low- flow deliveries. The flow is proportionate to the speed of the pump head and the inside diameter of the tubing both of which can be varied. A variable speed flow control and five different sized tubing assemblies provide fine resolution with a wide range of flow rates. Mini-Pump is powered by a 12-volt wall adapter that plugs into a wall receptacle. The dimensions are 16.8 x 12.0 x 11.5 cm. The flow rate range is 0.4 to 85.0mlminute. Partner with gradient makers listed above for pouring gradient gels. Gradient Makers Mini-Peristaltic Pump ORDERING INFORMATION CAT. DESCRIPTION GRADIENT MAKERS GM-20 Linear Gradient Maker 10mlsside with side outlet GM-40 Linear Gradient Maker 20mlsside with side outlet GM-100 Linear Gradient Maker 50mlsside with side outlet GM-150 Linear Gradient Maker 75mlsside with side outlet GM-200 Linear Gradient Maker 100mlsside with side outlet GM-500 Linear Gradient Maker 250mlsside with side outlet GM-1000 Linear Gradient Maker 500mlsside with side outlet GM-2000 Linear Gradient Maker 1000mlsside with side outlet ORDERING INFORMATION CAT. DESCRIPTION MINI-PERISTALTIC PUMP MPP-100 Mini-Peristaltic Pump 110V MPP-100-220 Mini-Peristaltic Pump 220V The C.B.S. SCIENTIFIC culture chambers are designed to be used within an incubator or warm room for a variety of tissue culture techniques. These durable hermetically sealed chambers are precision-fabricated from 38 acrylic and come with either 3 Cat. M-312 or 6 Cat. M-624 heavy gauge perforated aluminum shelves. The shelves are black anodized for corrosion resistance and provide a high contrast background for visualizing cell suspensions or monolayers. The smaller 3 shelf chamber has ouside dimensions of 11.75w x 4.6h x 9d. The larger 6-shelf chamber shown above has outside dimensions of 11.75w x 6.25h x 9d. FEATURES Saves on expensive gas mixtures eliminates need for continuous-flow CO2 incubators. Provides stable culture conditions by minimizing fluctuations in temperature pH and humidity. Incubator Culture Chambers ORDERING INFORMATION CAT. DESCRIPTION TISSUE CULTURE BOXES M-312 Tissue Culture Box 3 shelves M-624 Tissue Culture Box 6 shelves SPECIFICATIONS Chamber Cat. M-312 M-624 Number of Shelves 3 6 Outside Dimensions W x H x D cm 29.8 x 11.8 x 22.9 29.8 x 15.8 x 22.9 CapacityChamber 35mm diameter petri dishes 72 144 CapacityChamber 60mm diameter petri dishes 33 66 CapacityChamber 100mm diameter petri dishes 33 66 CapacityChamber 96-well microtitre plates 12 24 CapacityChamber 250ml culture flasks 3 6 APPLICATIONS Hybridomas Lymphocyte Cultures Short Term Organ Cultures Established Cell Lines Bacterial Cultures Virus-infected Cultures w w w. c b s s c i e n t i f i c . c o m80 LAB EQUIPMENT Beta Radiation Safety Equipment Conical Tube Radiation Containers ORDERING INFORMATION CAT. DESCRIPTION CONICAL TUBE RADIATION CONTAINER RC-50004 4-Place Conical Tube Radiation Container with hinged lid for 50ml tubes RC-15004 4-Place Conical Tube Radiation Container with hinged lid for 15ml tubes Eppendorf Tube Radiation Containers ORDERING INFORMATION CAT. DESCRIPTION EPPENDORF TUBE RADIATION CONTAINER RC-00563 63-Place Eppendorf Tube Radiation Container with hinged lid fits 63 each 0.5ml eppendorf tubes RC-01524 24-Place Eppendorf Tube Radiation Container with hinged lid. fits 24 each 1.5ml eppendorf tubes ORDERING INFORMATION CAT. DESCRIPTION EPPENDORF TUBE RADIATION CONTAINER RC-00524 24-Place Eppendorf Tube Radiation Container with hinged lid fits 24 each 0.5ml eppendorf tubes RC-01512 12-Place Eppendorf Tube Radiation Container with hinged lid fits 12 each 1.5ml eppendorf tubes ORDERING INFORMATION CAT. DESCRIPTION CONICAL TUBE RADIATION CONTAINER RC-15008 8-Place Conical Tube Radiation Container with hinged lid fits 8 each 15ml conical tubes RC-50008 8-Place Conical Tube Radiation Container with hinged lid fits 8 each 50ml conical tubes SPECIFICATIONS Radiation Container Cat. RC-00563 RC-01524 Outside Dimensions W x H x D cm 15 x 7.9 x 10.6 15 x 7.9 x 10.6 CapacityEppendorf Tube Size 630.5ml 241.5ml RC-00563 RC-01524 SPECIFICATIONS Radiation Container Cat. RC-00524 RC-01512 Outside Dimensions W x H x D cm 15 x 7.9 x 7.4 15 x 7.9 x 7.4 CapacityEppendorf Tube Size 240.5ml 121.5ml RC-00524 RC-01512 SPECIFICATIONS Radiation Container Cat. RC-15008 RC-50008 Outside Dimensions W x H x D cm 10.6 x 14.9 x 10.8 10.6 x 14.9 x 10.8 CapacityConical Tube Size 815ml 850ml SPECIFICATIONS Radiation Container Cat. RC-50004 RC-15004 Outside Dimensions W x H x D cm 17.1 x 14.9 x 10.8 17.1 x 14.9 x 10.8 CapacityConical Tube Size 450ml 415ml RC-50008RC-15008 RC-50004 RC-15004 p h o n e 8 5 8 7 5 5 - 4 9 5 9 To l l F r e e 8 0 0 2 4 3 - 4 9 5 9 81 LAB EQUIPMENT Radiation Waste Receptacles Quick access lid left and locking hardware right can be added to most of the radiation safety containers or receptacles at time of order. ORDERING INFORMATION CAT. DESCRIPTION RADIATION WASTE RECEPTACLES RF-100C Large Capacity Radioactive Waste Container with hinged lid RF-100LC Large Capacity Radioactive Waste Container with hinged lid and locking casters RF-100IB Cardboard Insert Box for RF-100C for ease in waste disposal RB-100 Mini Bench-Top Radioactive Waste Receptacle with hinged lid RG-100 Bench-Top Radioactive Waste Receptacle with hinged lid Radiation container with hinged lid can hold one 80-Place Microfuge Tube Rack. Acrylic container dimensions are 5.1cmh x 22.8cmw x 6.7cmd. ORDERING INFORMATION CAT. DESCRIPTION MICROFUGE RACK RADIATION CONTAINER GTR-080 80-Place Microfuge Tube Rack holds 1.5ml microfuge tubes. Available in green yellow red and natural. RK-080 Microfuge Tube Rack with Lid hinged radiation container. 5.1cmh x 22.8cmw x 6.7cmd 80-Place Rack Radiation Container with Lid 96-place flip racks can hold 1.5ml or 2.0ml microfuge tubes on one side and be flipped over to accommodate 96 0.5mm - 0.65mm microfuge tubes on other side. Available in green red blue natural or yellow. Yellow rack is shown above inside Personal Carrier Shield This Personal Carrier permits mobility and protection without having to use a designated radioactive work station. The researcher can transport samples from bench to waterbath to freezer knowing the radioactive samples ares safely shielded from coworkers. A storage compartment beneath the samples can be filled with ice whenever necessary. Holds one 96-place microfuge rack. ORDERING INFORMATION CAT. DESCRIPTION MICROFUGE RACK RADIATION CONTAINER GTR-096 96-Place Flip Rack holds 0.5mm-0.65mm microfuge tubes on one side and 1.5ml-2.0ml microfuge tubes on other. Available in blue green yellow red and natural. RL-096 Personal Carrier Shield Beta radiation shield with hinged lid and ice trough for use with 96-Place Flip Racks. 96-Place Flip Racks and Personal Carrier Shield SPECIFICATIONS Radiation Container Cat. RF-100C RG-100 RB-100 Outside Dimensions W x H x D cm 33 x 73.6 x 33 20.3 x 30.5 x 20.3 12.7 x 17.8 x 12.7 RF-100LC shown with RF-100IB insert RG-100RB-100 Options Available -QAL Quick Access Lid Option shown above- top right for ease in disposal of small items. This factory installed option can be specified by adding -QAL to end of catalog number. -LH Locking Hardware Option shown above- bottom right. This factory installed option can be specified by adding -LH to end of catalog number. Factory Options GTR-096 RL-096 RK-080 and GTR-080 Microfuge Tube Rack w w w. c b s s c i e n t i f i c . c o m82 LAB EQUIPMENT The Radioiodination Hood is designed to be used inside existing fume hoods to minimize the iodine volatilization hazards present during radiolabelling procedures. Most standard laboratory fume hoods alone do not provide the adequate airflow proper ventilation nor the charcoal filtration necessary to sufficiently reduce radioactive iodine from evolving into the general air supply. This hood significantly reduces these hazards making it a simple cost effective way of upgrading an existing fume hood. Proper shielding such as lead or leaded acrylic is required inside of hood. Dimensions are 24w x 21d x 20h with high volume blower and 1 activated charcoal filter. ORDERING INFORMATION CAT. DESCRIPTION RADIOIODINATION HOOD IH-024 Radioiodination Hood with high volume blower and charcoal filter ICF-100 Activated Charcoal Filter SF-100 Air sampler for ICF-100 Charcoal Filter Radioiodination Hood Beta Radiation Safety Equipment Radiation Shields ORDERING INFORMATION CAT. DESCRIPTION RADIATION SHIELD RD-100 Radiation Shield with side supports 38.1h x 35.6cmw x 22.8cm angled back at 45 ORDERING INFORMATION CAT. DESCRIPTION RADIATION SHIELD RS-200 Radiation Shield with side cutout 35.4cmw x 54cmh x 26.1cmd. 22.8cm angled back at 45 www.cbsscientific.com Telephone Local or International 858-755-4959 Toll Free 800-243-4959 Fax 858-755-0733 E-mail address salescbssci.com Mailing address C.B.S. Scientific Company P.O. Box 856 Del Mar CA 92014 Corporate Office and Shipping Address C.B.S. Scientific Company 10805 Vista Sorrento Pkwy Suite 100 San Diego CA 92121 Contact Information 40Years 1973-2013 43Years 1973-2016